Department of Biological Sciences National University of Singapore Standard Operation Procedure

Title: Cleaning / Maintenance of Equipment and Work Areas in the Lab Lab: Cellular and Molecular Parasitology Lab
Written by Approved by Issue date

Ref. No Lab/SOP/004 Rev. No Page: 001 3

Review date

Dulani Gamlaksha

Dr. Cynthia He

March 15, 2010

1. Purpose
This SOP details the cleaning procedures for work areas and for some commonly used equipments in the lab.

2. Scope This SOP is applicable to all staff and students working in the lab. 3. Responsibility Primary responsible persons for the cleaning / maintenace of different equipments and areas are as below. But it is empathized that should any equipment or an area get dirty during the use by any lab member then prompt cleaning of that equipment or area shall fall on that particular person. Hence in effect keeping the equipment and work areas clean is the responsibility of all the members of the lab. Equipments: Guava flow cytometer and equipments in tissue culture room (Microscope and CO2 incubators) Wang Min Live cell imaging system and accessories Zhou Qing Centrifuges - Ladan Other small equipments in the lab (ex: Balances, pH meter, shakers, water bath etc) Zhang Yu DNA/protein blotting systems Sun Ying Fridges and freezers Dandan Fumehood Fern Areas

Gel electrophoresis area Sun Ying Chemical area Sink area Fern Bench space Individual person to whom each bench space is designated

Department of Biological Sciences National University of Singapore Standard Operation Procedure

Title: Cleaning / Maintenance of Equipment and Work Areas in the Lab Lab: Cellular and Molecular Parasitology Lab
Written by Approved by Issue date

Ref. No Lab/SOP/004 Rev. No Page: 001 3

Review date

Dulani Gamlaksha

Dr. Cynthia He

March 15, 2010

Other general lab areas - Dulani

4. Procedures For Equipments: 4.1. Before proceeding to clean any equipment make sure that they are discontinued from the power supply 4.2. For general equipment, depending on the type of equipment and suitability it is recommended to wipe the equipment with 0.1% bleach solution (if the equipment is compatible with bleach) and then with 70% ethanol using paper wipes. 4.3. For specialized equipment like the flow cytometer use recommended cleaning fluids for maintenance and cleaning. 4.4. For freezers first allow them to defrost. Before turning off the power the things inside the freezers need to be changed to another designated freezer. Put on double gloves or use oven gloves for this transfer as the surfaces would be very cold. Clearly label the freezer to which the things have been temporarily transferred and let everyone know about the transfer. For Work Areas: 4.1. For electrophoresis area bench lining need to be changed as and when necessary 4.2. For chemical area the lining paper need to be changed as and when necessary 4.3. Only the sink closer to the fumehood is supposed to be used for washing of laboratory material. The flasks containing bacterial cultures should be added sufficient volumes of bleach and allowed to decontaminate for a proper length of time. These flasks should be promptly removed by the respective users to prevent cluttering of the sink area. All the other items that need to be washed too should be washed promptly and should not be allowed to accumulate in the sink area. Items that are left to dry too should be promptly removed after drying. Sink closer to the entrance is strictly used only for hand washing.

Department of Biological Sciences National University of Singapore Standard Operation Procedure

Title: Cleaning / Maintenance of Equipment and Work Areas in the Lab Lab: Cellular and Molecular Parasitology Lab
Written by Approved by Issue date

Ref. No Lab/SOP/004 Rev. No Page: 001 3

Review date

Dulani Gamlaksha

Dr. Cynthia He

March 15, 2010

4.4. Bench spaces should be regularly cleaned and wiped with 70% ethanol on a regular basis 5. Safety precautions Make sure the equipments are not in use and have been discontinued from power before proceeding to clean them Always wear gloves, lab coats and covered shoes When cleaning freezers make sure lab coats are worn and extra protection is given for hands wearing double gloves or oven gloves as the surfaces would be very cold