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  • MacConkey Agar

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    Ashiya Arie
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    MacConkey agar is a moderately selective culture medium used to detect coliform bacteria and enteric pathogens. It inhibits the growth of gram-positive bacteria through the use of crystal violet and bile salts. Common bacteria that can grow on MacConkey agar include Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae, which form pink or rose-red colonies. Salmonella, Shigella, Proteus, and others form colorless colonies.

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    MacConkey agar is a moderately selective culture medium used to detect coliform bacteria and enteric pathogens. It inhibits the growth of gram-positive bacteria through the use of crystal violet and bile salts. Common bacteria that can grow on MacConkey agar include Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae, which form pink or rose-red colonies. Salmonella, Shigella, Proteus, and others form colorless colonies.

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    40 views1 page

    MacConkey Agar

    Uploaded by

    Ashiya Arie
    MacConkey agar is a moderately selective culture medium used to detect coliform bacteria and enteric pathogens. It inhibits the growth of gram-positive bacteria through the use of crystal violet and bile salts. Common bacteria that can grow on MacConkey agar include Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae, which form pink or rose-red colonies. Salmonella, Shigella, Proteus, and others form colorless colonies.

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    MacConkey Agar Use: Moderately selective culture medium for the detection of coliform organisms and enteric pathogens

    GROWTH INHIBITION OF GROWTH LACTOSE POSITIVE examples


    Escherichia coli (most strains) Klebsiella pneumoniae Entrobacter cloacae

    LACTOSE NEGATIVE examples


    Salmonella enterica ssp. enterica Shigella spp. Proteus spp. Citrobacter freundii (some strains) Morganella morganii Providencia spp. staphylococci streptococci enterococci

    Crystal violet and bile salts in medium inhibit growth of Gram-positive microorganisms BACTERIUM Escherichia coli Enterobacter cloacae Klebsilla pneumoniae Proteus Pseudomonas aeruginosa Salmonella and Shigella Gram-positive bacteria TYPICAL GROWTH ON MACCONKEY AGAR Pink to rose-red. Colonies may be surrounded by a zone of precipitated bile. Pink, mucoid. Pink, mucoid. Colorless. Colorless to pink. Colorless. No growth to slight growth (pale pink).

    http://www.bacteriainphotos.com/agar%20cultivation%20media.html

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