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    Cultivation, Collection and Preparation of Ergot A4

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    dhimant_09
    The document describes the life cycle and cultivation of the ergot fungus. It has three stages: 1) The sphacelial stage where ascospores infect rye ovaries under favorable conditions like moisture. The fungus produces a soft white mycelium and honeydew. 2) The sclerotium stage where the fungus invades deeper and replaces the ovary tissue, forming hard dark sclerotia that contain ergot alkaloids. 3) The ascospore stage where uncollected sclerotia produce fruiting bodies containing ascospores that can infect new rye plants. Saprophytic production in fermentation is now commonly used to isolate and purify alkaloids, as the fungus yields over

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    Cultivation, Collection and Preparation of Ergot A4

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    dhimant_09
    2K views3 pages
    The document describes the life cycle and cultivation of the ergot fungus. It has three stages: 1) The sphacelial stage where ascospores infect rye ovaries under favorable conditions like moisture. The fungus produces a soft white mycelium and honeydew. 2) The sclerotium stage where the fungus invades deeper and replaces the ovary tissue, forming hard dark sclerotia that contain ergot alkaloids. 3) The ascospore stage where uncollected sclerotia produce fruiting bodies containing ascospores that can infect new rye plants. Saprophytic production in fermentation is now commonly used to isolate and purify alkaloids, as the fungus yields over

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    The document describes the life cycle and cultivation of the ergot fungus. It has three stages: 1) The sphacelial stage where ascospores infect rye ovaries under favorable conditions like moisture. The fungus produces a soft white mycelium and honeydew. 2) The sclerotium stage where the fungus invades deeper and replaces the ovary tissue, forming hard dark sclerotia that contain ergot alkaloids. 3) The ascospore stage where uncollected sclerotia produce fruiting bodies containing ascospores that can infect new rye plants. Saprophytic production in fermentation is now commonly used to isolate and purify alkaloids, as the fungus yields over

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    2K views3 pages

    Cultivation, Collection and Preparation of Ergot A4

    Uploaded by

    dhimant_09
    The document describes the life cycle and cultivation of the ergot fungus. It has three stages: 1) The sphacelial stage where ascospores infect rye ovaries under favorable conditions like moisture. The fungus produces a soft white mycelium and honeydew. 2) The sclerotium stage where the fungus invades deeper and replaces the ovary tissue, forming hard dark sclerotia that contain ergot alkaloids. 3) The ascospore stage where uncollected sclerotia produce fruiting bodies containing ascospores that can infect new rye plants. Saprophytic production in fermentation is now commonly used to isolate and purify alkaloids, as the fungus yields over

    Copyright:

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    Cultivation, collection and preparation of Ergot

    Life cycle of Ergot


    Stage 1: Sphacelial stage Germination of ascospores Ovary of the rye plant (Base) Influenced by Flavourable conditions: wind and insects moisture, damp climate.

    Infected by ascospores of the fungus in spring or summer season Hyphal strands Secrection of Honey-dew Hyphal strands Invading the wall of ovary ( by enzyme) Mycelium (soft, white mass of tissue over the surface of ovary) Secrets Honey-dew (viscous and sugary fluid) (attracts the insects and ants which leads to spread of conidiospores to other host plant) Stage 2: Sclerotium stage (contains maximum amount of ergot alkaloids) Hyphal strands
    Invade into the deeper parts of ovary

    Produced asexual spores conidiospores (remains in Honey-dew)

    Slowly replace the entire tissue of ovary by compact tissue (pseudoparenchyma) (hard and dark purple)

    Sclerotium (collected)

    NOBLE PHARMACY COLLEGE, JUNAGADH

    Stage 3: Ascospore stage Uncollected Sclerotium (on ground) in favourable season (spring) gives out Stromata Stromata: Globular head contains perithecia (flask shaped) and Sacs contain ascospores (thread like) A Stalk The various chemical races of fungus can produce only specific ergot alkaloids like ergotamine, ergometrine and ergotoxin in appreciable quantities from their sclerotia. The ascospores of this spices with the specific chemical race are germinated on nutritive medium and by this way large bulk of conidiospores are formed. The suspension of this strain of fungus is sprayed on rye plants in large cultivated areas.

    Saprophytic Production:
    Fermentation: Fungus and nutrients pH : 4.6 6.3, Temp.: 20 - 30C 7 to 21 days Isolation, Separation and Purification. e.g. Claviceps paspali (fungus) gives clavines and simple lysergic acid derivatives. It is much easier to manufacture clavines and simple lysergic acid derivatives and then convert them to different peptides alkaloids (ergot alkaloids). The Saprophytic Production is much practices now-a-days because mycelia dry weight gives even more than 20 % of alkaloids, while natural sclerotia contain less than 1% of alkaloids.

    NOBLE PHARMACY COLLEGE, JUNAGADH

    NOBLE PHARMACY COLLEGE, JUNAGADH

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