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แผนปฏิบัติการเพื่อการวิจัยพันธุวิศวกรรมพืช
แผนปฏิบัติการเพื่อการวิจัยพันธุวิศวกรรมพืช
.............................................................................................2
.4
.....................12
1 ..16
2 ....................................................................................30
4.
2
1 2
(transformation)
2
Agrobacterium (particle bombardment)
3 ( 1)
1
(
)
2
1
1
/
Gene stacking
New selectable maker
Novel promoter
promoter
35S promoter tissue specific promoter
inducible promoter
(abiotic stress)
Chloroplast transformation
positional effects
Maternal inheritant
(pollen) chloroplast
(gene flow)
( 2) 3
1
Pox
Virus),
1.3
(Defense mechanism)
hypersensitive signal molecule
ferredoxin-like protein (pflp)1 wasabi defensin protein Erwinia carotovora,
thaumatin Pythium aphanidermatum Rhizoctonia solani, germin protein (oxalate
oxidase) Sclerotinia chitinase
Phalaenopsis Oncidium
2. Abiotic stress
(drought stress),
(salt stress), (cold stress) transcription
factor, signal transduction component gene, transporter protein gene
osmolyte SacB (Levan sucrase; Bacillus subtilis) fructan
P5CS (-Pyrroline-5-carboxylate synthase)
proline
biotic abiotic stress mtID
(Mannitol-1-phosphate dehydrogenase fungal wilts Escherichia coli
3. (Herbicide tolerance)
(Doane Market
Research, .. 2000)
bar (phosphinothricin-N-acetyltransferase), EPSPS (3-enolpyruvyl shikimic acid5-phosphate synthase) 2, 4-D monooxygenase
4. (Quality improvement)
4.1
Phosphoenolpyruvate carboxylase (pepc) C4 C3
4.2
(Ripening and senescence hormone) antisense RNAi ACC
synthase ACC oxidase2
ethylene receptor (ETR1) ethylene receptor
4.3
(Golden rice)
Erwinia uredovara (Japonica rice) (indica rice)
stacking gene ( feritin , phytate
cysteine )
(antioxidant)
-carotene 3 lycopene rapeseed
() ()
trans ( streptomyces-3hydroxysteroid oxidase phytosterols phytostanols) (forage crop)
(lignin) antisense
4.4
(Florigene)
(Suntory)
antisense
2 (Energy plant)
duck weed
4
1)
4) palmetic acid
oleic acid
3 (Biopharmaceutical farming)
3
1. Edible vaccines
herpes, , , AIDS,
, chlorela toxin B
(Streptococcus mutants)
2. Plantibodies
antibody
antibody anti-mouse major histocompatibility complex
(MHC) class II, immunoglobulin for dental caries IgG1(slgA-G), tumor-associated marker antigen (scFv
T84.66 against carcinoembryogenic antigen antibody
(plantibody factories) antibody
(heavy chain, light chain) antibody
antibody human -(1, 4)galactosyltransferase (-GalT linker gene)4
antibody regenerate
3. (Pharmaceutical protein)
Human protein C (serum protease), Human hirudin, N-acetyl-neuraminic acid, Human secreted
alkaline phosphatase, Human serum albumin, Human epidermai growth factor
safflower duckweed
2
1) (Direct expression/ stable transformation)
() (constitutive expression)
(seed specific expression)
chloroplast chloroplast
chloroplast
homologous pollen chloroplast
2) (transient transformation)
(mammalian and insect cell culture)
10 100
histidine tag elastin-like polypeptide (ELP)
(Rhizosecretion) (Phytoguttation)
4 (Industrial plant)
1.
keratin
2.
(biodegradable plastic)
polyhydroxyalkanoate (PHA) polyhydroxybutyrate (PHB)
Arabidopsis
10
3.
loblolly pine silver birch
4.
.. 2004
protein base
material fibrous glues, human elastin
amylase
biotic abiotic stress
5 (Environmental application)
(phytoremediation)
phytoremediation
ACC deaminase
nitroreductase Trinitrotoluene (TNT)
Cyclotrimethylenetrinitramine (RDX)
hairy root
11
1
1.
2.
3.
2
(marker-free)
1
4.
12
5.
6.
7.
8.
()
2
1
(model)
2
(high
value product) 1
13
biomass
Edible vaccine
Edible vaccine
fiber
yield
yield
fiber
regeneration
transformation
yield
(
Arabidopsis
)
Waxy cassava
yield 9
(
-
process)
GM
tissue culture
biomass
latex
14
.. 2551 2554
3
3
1 (3 - 5 )
3
( )
2 (5-10 )
3 (10 )
15
promoter
Selectable
marker
regenerate
1
Nemesia
strumosa
Oncidium
U. of
Tsukuba
/ Japan
Institute of
BioAgricult
ural
Sciences
/ Republic
of China
N. strumosa GM
missense mutation
melon ethylene receptor
gene (Cm-ETR1)
ethylene signal transduction
GM
ferredoxin-like
protein (pflp)
Erwinia carotovora
melon
ethylene
receptor
gene
CaMV35S
nptII gene
Stem
/A.tumefaciens
strain GV2260
N/A
promoter
2x
4x CaMV35S
floral specific promoter
ethylene
risk assessment
hpt gene
Protocorm
Protocormliked
bodies (PLBs)
/
3.4%
ferredoxin-like protein
E. carotovora
selectable marker
PLBs
selectable marker
Liau et al.,
2003
CmETR1/H69A
Pflp gene
CaMV35S
A. tumefaciens
strain
EHA105
You et al.,
2003
16
Phalaenopsis
Chiba U.
/japan
GM wasabi
defensin protein
Erwinia carotovora
wasabi
defensin
gene /
promoter
Selectable
marker
CaMV35S
hpt gene
Wasabia
japonica
Embryogenic
cell suspension
/
regenerate
78 %
wasabi
defensin
risk assessment
Sjahril et al.,
2006
A. tumefaciens
strain EHA101
U. of
(Microprojectile
bombardment)
bar gene
CaMV35S
bar gene
PLBs / particle
bombardment
10 %
risk assessment
Knapp et al.,
2003
National
Taiwan U.
/ Taiwan
GM CymMV
coat protein pepper
ferredoxin-like protein
(gene stacking)
CymMV coat
protein
cDNA (CP)
sweet
pepper
ferredoxinlike protein
cDNA (Pflp)
Ubiquitin
CaMV35S
hpt gene
PLBs/ particle
bombardment
N/A
risk assessment
Chan et al.,
2005
GM
CymMV coat
protein
Cymbidium
Mosaic Virus
CymMV coat
protein
cDNA (CP)
/Cymbidium
CaMV35S
hpt gene
PLBs/ particle
bombardment
N/A
risk assessment
Chang et al.,
2005
GM
CymMV coat
protein
Cymbidium
Mosaic Virus
CymMV coat
protein
cDNA (CP)
/Cymbidium
Maize
Ubiquitin
promoter
hpt gene
Protocormliked
bodies (PLBs)/
particle
bombardment
N/A
risk assessment
Chan and
Chan, 2005
Brassia,
Rhode
Cattleya,
Island
Doritaenopsis /USA
Phalaenopsis
carotovora
Dendrobium
Phalaenopsis
CH835
National
Taiwan U.
/ Taiwan
National
Taiwan U.
/ Taiwan
A.
tumefaciens
strain EHA101
sinesis
sinesis
17
IRRI
(IR68899B)
Gen. &
Biochem.
Divis. /
Philippines
GM (Bt-rice)
stem borer
cryIA(b) /
Bacillus
thuringiensis
Taipei 309
hybrid cv.
H62-4671
U. of
Hawaii/
USA
sugarcane polyubiquitin
promoters 2
maize Ubi-1 promoter
ubi9
ubi4
ubi9
MAR
(Flanking Nuclear Matrix
Attachment Regions)
construct ubi9
GM
Phosphoenolpyruvate
carboxylase (pepc)
CO2
C4
Gus gene
Indica rice
U. of
Calcutta,
India
Agriculturl
maize pepc
gene
promoter
CaMV
35S
sugarcane
polyubiquitin
promotersub
i4 ubi9
maize Ubi-1
promoter
maize pepc
gene
promoter
Selectable
marker
cotransformed
with plasmid
pGL2
(hygromycin
phosphotransf
erase gene)
hygromycire
sistance(hpt)
gene
Kanamycin
resistance
(nptII gene)
hpt gene
nptII gene
regenerate
Embryogenic
calli /
Bombardments
20%
risk assessment
M. F. Alam, et
al.,1999
()
mature seed
scutellum/
N/A
promoter
promoter
target genes
Heat
stress tolerance ubi
4
Wei et al.,
2003
N/A
Bandyopadhyay
et al., 2007
A. tumefaciens
strain EHA105
()
embryogenic
callus / particle
bombardment
Embryo genic
calli / biolistic
transformation.
18
Bt-Indica
Basmati rice
B-370
U. of
Punjab
/Pakistan
4 inbred
lines
Chinese
Academy
of Sci./
China
Chinese
Aca.of Agr.
Sci./ China
Eggplants
U. of DelhiSouth
Campus/
India
promoter
Selectable
marker
regenerate
cry1Ac
cry2A genes
CaMV 35S
N/A
N/A
N/A
gus-intron
(uidA-intron)
/E. coli
CaMV35S
bar gene
risk assessment
Bt-Indica Basmati rice
B-370
Agrobacterium
GM
DC-ACS1
/ carnation
(sACS
2,4-D
GM 2,4-D
monooxygenase (tfdA) gene
GM
mannitol
abiotic stress (
)
biotic stress (
)
2,4-D
monooxygen
ase (tfdA)
gene
Mannitol-1phosphate
dehydrogena
se (mtlD)/
(phosphinot
hricin
acetyltransfe
rase gene;
PPTresistant)
CaMV35S
hpt gene
nptII gene
risk assessment
Bashir et al.,
2005
2.59-4.20%
Xueqing Huang
& Zhiming Wei,
2005
A. tumefaciens
N/A
ethylene
risk assessment
Iwazaki et al.,
2004
N/A
risk assessment
Zhang et al.,
2001
32-73 %
abiotic stress
biotic stress
mtlD
risk assessment
Prabhavathi et
al., 2002
Immature
Embryos / A.
tumefaciens
strain EHA105,
LBA4404
and nopaline
GV3101
strain EHA101
transgene
aACS
transgene)
E. coli
CaMV35S
nptII gene
N/A
CaMV35S
nptII gene
Leaf disc/
A. tumefaciens
strain
LBA4404
Prabhavathi
and Rajarn,
2007
19
promoter
Leaf strips/
N/A
risk assessment
Millar et al.,
2007
A. tumefaciens
N/A
ethylene
risk assessment
Xiong et al.,
2005
N/A
N/A
risk assessment
leaf discs /
A.tumefaciens
N/A
risk assessment
Urwin
et al., 2001
U. of
London/
UK
GM
cinnamate
4-hydroxylase (CYP73A24)
antioxidant (rutin
naringenin)
sense
CYP73A24
CaMV35S
nptII gene
Shanghai
Academy
of Agri.
Sci./ China
GM
1Aminocyclopropane-1carboxylate oxidase (ACO)
RNA interference
(RNAi)
ethylene
RNAi
ACO gene
CaMV35S
hpt gene
U. of
Leeds/ UK
GM
protease
inhibitor 2
leafhopper
U. of
Leeds/ UK
chicken egg
white
cystatin
(CEWc)
N/A
Kanamycin
resistance
A. tumefaciens
strain LBA4404
(Neomycin
phosphotran
sferase
gene; nptII)
and
oryza
cystatin
I (Oc-I_D86)
cysteine
proteinase
inhibitor
regenerate
Selectable
marker
CaMV35S
(constitutive
promoter)
N/A
LBA4404
20
Shallots
promoter
Selectable
marker
wound-inducible (win3.12)
Gus (uidA)
reporter
gene/ hybrid
poplar
woundinducible
(win3.12)
promoter
nptII
U. of Delhi
/ India
GM
thaumatin
Pythium
thaumatin
gene /
CaMV35S
nptII gene
aphanidermatum and
Rhizoctonia solani
U. of
Victoria
/Canada
Monsanto
Company
/USA
Petioles/
A.tumefaciens
regenerate
100%
regeneration
strain MP90
Thaumatoco
ccus daniellii
GM
Streptomyces
3Streptomyces 3hydroxysteroi
hydroxysteroid oxidase
d oxidase
gene
phytosterols
/
S.hygroscopi
photostanols
cus
photostanols
LDL cholesterol
serum phytosterols
Wagening
GM
Cry1Ca
U./
Bt-toxin
H04 /
B.thuringiensis
Natherlands
chrysanthemum Rubissco
small subunit promoter
beet armyworm
100 %
Samuel
Sense
Roberts
sense antisense
Noble
antisense 2
COMT
Found./
lignin
USA
Alfalfa antisense
leaf disc/
Agrobacterium
N/A
strains
LBA4404
napin
promoter
chloroplast
targeting
signal
sequence
Rubissco
SSU
promoter
nptII gene
Bean
promoter
thaumatin
risk assessment
risk assessment
Yevtushenko et
al., 2004
Rajam et al.,
2007
hypocotyls
(B. napus )
Cotyledon
(G. max) /
N/A
Venkatramesh
Callus from
mature
embryos /
3.68 %
bulbs risk
assessment
Zheng et al.,
2005
N/A
lignin
Guo et al.,
2001
et al., 2002
A.tumefaciens
hpt gene
A.tumefaciens
strain AGL0
(constitutive
promoter)
Phenylalanin
e ammonialyase (PAL2
promoter)
Bar gene
Embryogenic
calli /
A.tumefaciens
21
Royal Gala
Samuel
Roberts
Noble
Found./
USA
Samuel
Roberts
Noble
Found./
USA
Victorian
AgriBiosci.
Centre
/ Australia
/
methyl- transferase (COMT)
s lignin
caffeoyl coenzyme 3-Omethyltransferase
(CCoAOMT)
G lignin
Cytochrome P450
antisense 3
lignin
Alfalfa
cinnamate
4-hydroxylase (C4H),
coumaroyl shikimate 3hydroxylase(coumarate 3hydroxylase ; C3H)
coniferaldehyde 5hydroxylase (ferulate 5hydroxylase; F5H)
antisense
hydroxycinnamoyl CoA :
Shikimate hydroxycinnamoyl
transferase (HCT)
lignin
GM
proteinase inhibitor (PI)
promoter
CCoAOMT
vascular
tissue
specific
promoter
antisense
C4H (M.
sativa), C3H
(Arabidopsis)
F5H (M.
sativa)
PAL2
antisense
HCT /
alffalfa
PAL2
/ alffalfa
proteinase
inhibitor
(PI)/
N. alata
Selectable
marker
regenerate
nptII gene
Leaf disc
/A.tumefaciens
strain GV3101
N/A
lignin
risk assessment
Reddyet al.,
2005
Leaf disc /
N/A
lignin
risk assessment
proteinase
inhibitor
risk assessment
Shadle et al.,
2007
Bean
vascular
tissue
specific
promoter
bar gene
A.tumefaciens
strain GV3101
Bean
vascular
tissue
specific
promoter
2x CaMV35S
risk assessment
promoter
promoter
nptII gene
leaf disc /
Agrobacterium
strains
LBA4404
CZ707
2.86 % (strains
LBA4404)
0.55 % (strains
CZ707)
Maheswaranet
al., 2007
22
promoter
Selectable
marker
regenerate
Duckweed
U. of
GM
Kebangsaan stem borer
Malaysia
/Malaysia
cryIA(b)
Wageningen GM
U./ Nether granule-bound
lands
starch synthase I (GBSSI)
antisense
amylose-free starch
North
GM
Carolina
Endoglucanase (E1)
State U.
Acidothermus
cellulolyticus
/USA
Antisense
GBSSI
gene
/ B.
rubisco
promoter
hpt gene
Immature
Embryos/
Bombardments
81% transient
expression
nptII gene
Friable
embryogenic
callus
/ Particle
Bombardment
N/A
nptII gene
A. tumefaciens
N/A
thuringiensis
Endoglucana
se (E1)
/Acidotherm
us
cellulolyticus
CaMV35S
CaMV35S
Immature
Embryos
variegated
Raemakers
et al., 2005
CaMV35S
E1
overxepression
E1
duckweed GM
Sun et al.,
2007
23
U.de de
So Paulo
/Brazil
GM
soybean proteinase
inhibitors 2
SBBI SKTI
sugarcane
borer Diatraea saccharalis
Louisiana
State U.
Agr.l
Center
/ USA
rice
ubiquitin promoter (RUBQ2)
maize Ubi-1
promoter CaMV35s
promoter
RUBQ2 promoter
gus
soybean
proteinase
inhibitors
Gus gene
promoter
Selectable
marker
regenerate
maize
ubiquitin
promoter
(Ubi-1)
nptII gene
Embryogenic
callus /particle
bombardment
N/A
risk assessment
Falco
and Silva-Filho,
2003
rice ubiquitin
promoter
(RUBQ2)
nptII gene
Embryogenic
callus / A.
tumefaciens
strain LBA
4404 and AGL1
15%
promoter
anti MHC
class II monoclonal
antibody
GM
plantiboby
plantiboby
GM (GT6)
-GalT
Hong et al.,
2007
Chonbuk
National
U./ Korea
anti-mouse major
histocompatibility complex
(MHC) class II monoclonal
antibody GM
Anti-MHC
class II
heavy and
light chain
gene
/ mouse
CaMV35S
hpt gene
Leaf disc/
A. tumefaciens
strain LBA4404
N/A
Osaka U./
Japan
GM
mouse monoclonal antibody
stacking gene
(GT6)
human
-(1,4)-galactosyltransferase
(-GalT)
heavy and
light chain
gene
/ mouse
CaMV35S
hpt gene
Cell
suspension/
A. tumefaciens
strain
LBA4404
N/A
Fujiyama et
al., 2007
24
Bright
Yellow 2
(BY2)
Universit
de Rouen
heavy
light chain mouse
monoclonal antibody
Fc
region Heavy chain
-GalT
GM
terminal sialic
acids N-linked
glycans
(recombinant
pharmaceutical proteins)
halflife
/France
Rutgers U.
/ USA
Arizona
State U.
/USA
GM
human
secreted alkaline phosphatase
(SEAP) hairy root
adventitious
transgenic roots 57
GM
particle
2 hepatitis B
surface antigen (HBsAg)
Norwalk virus
capsid protein (NVCP)
Neu5Ac
lyase / E.
coli
promoter
Selectable
marker
CaMV35S
nptII gene
Neu5Ac
synthase
(neuB2) /
C.jejuni
human
secreted
alkaline
phosphatase
(SEAP)
HBsAg
NVCP
CaMV35S
nptII gene
2X CaMV 35S
promoter
5
UTR
tobacco etch
virus
nptII gene
()
suspensioncultured cells/
A. tumefaciens
strain LBA4404
regenerate
N/A
recombinant
pharmaceutical proteins
GM
Paccalet
et al., 2007
A. rhizogenes
N/A
Rhizosecretion
biopharmaceutical
plant
root specific
promoter rol
constitutive
promoter
Gaume et al.,
2003
Leaf disc/
N/A
plant
vaccines
risk assessment
Huang et al.,
2005
()
suspensioncultured cells /
A. tumefaciens
strain AGL-1
A. tumefaciens
strain
LBA4404
25
promoter
Selectable
marker
regenerate
N/A
N/A
Drake et al.,
2003
N/A
chloroplast
Patel et al.,
2007
Guys
Hospital
/UK
murine
monoclonal IgG1
Rhizosecretion
hydroponic
gelatin
gelatin
IgG
murine
monoclonal
IgG1
N/A
N/A
Agr. &
AgriFood
Canada
/ Canada
GM
elastin-like
polypeptide (ELP)
thermally responsive tags
(pIL4-HIS,
pIL4-ELP,
pIL10-HIS,
pIL10-ELP,
MaSp2-ELP
2x CaMV35S
N/A
Leaf discs/
A. tumefaciens s
4
Silver brich
(Betula
pendula
U.of
Helsinki/
Finland
Roth)
loblolly pine
East
Carolina
University/
USA
Silver
brich GM
4-coumarate :
coenzyme A ligase (4CL)
2 leaf
litter Mycorrhiza
GM
mannitol-1-phosphate
dehydrogenase (Mt1D)
glucitol-6-phosphate
dehydrogenase (GutD)
antisense
4CL / brich
CaMV35S
Mt1D
GutD
construct
CaMV35S
nptII gene
A. tumefaciens
N/A
risk assessment
Seppanen et
al., 2007
nptII gene
Cullus
mature zygotic
embryos /
6.214.1%
risk assessment
Tang et al.,
2005
strain C58C1
A. tumefaciens
strain LBA
4404
26
ChristianAl
brecht U./
Germany
/
GM
spider silkelastin fusion proteins
4%
total soluble protein
synthetic
spider silk
protein
(SO1)
elastic
biopolymer
100xELP
promoter
Selectable
marker
CaMV35S
nptII gene
Leaf discs/
A. tumefaciens
regenerate
N/A
N/A
Scheller et al.,
2004
strain 2260
indica rice
cv. IR64
Plant Mol.
& Cell.
Gen./
India
U. of
Waterloo/
Canada
U. of
Waterloo/
Canada
GM
Allium sativum leaf
lectin (asal ) RNA
sap sucking insect
pests (brown planthopper
and green leafhopper)
T0
T1
canola
ACC
deaminase
GM ACC deaminase
Mannose
binding
Allium
sativum leaf
agglutinin
(Actin,
ASAL)
Gene/
Allium
sativum
ACC
deaminase/
Pseudomona
s putida
CaMV35S
hpt gene
Cullus from
immature
seed/
12.1 %
risk assessment
Cullus/
N/A
Cullus/
N/A
canola
ACC deaminase
rolD
promoter
canola
risk assessment
A. tumefaciens
strain EHA105
CaMV35S
N/A
rolD (root
specific
promoter)
ACC
rolD (root
deaminase / specific
Pseudomonas promoter)
putida UW4
N/A
Agrobacterium
tumefaciens
A.tumefaciens
Prasenjit Saha
et al., 2006
Stearns et al.,
2005
Farwell et al.,
2007
27
Nat.
Botanical
Res. Ins./
India
cry1EC/
Bacillus
thuringiensis
U. of
Waterloo/
Canada
GM
hybrid endotoxin
polyphagous lepidopteran
pest
promoter 3 promoter
2x CaMV 35S, root specific
expression (rolD)
pathogenesis related (PRB1b)
(phytoremediation)
ACC
deaminase
U. of
Ottawa/
Canada
ACC
deaminase/
Pseudomona
s putida
Hebrew U.
of
Jerusalem
/Israel
CaMV35S
nptII gene
CaMV35S
N/A
regenerate
cry2Aa2/
(whiteflies)
GM bglucosidase gene (BGL1)
Aspergillus
niger bglucosidase
(EC
3.2.1.21)
gene (BGL1)
Bacillus
thuringiensis
nptII gene
homologous,
single copy T2
risk
assessment
Singh et al.,
2004
A. tumefaciens
N/A
Grichko et al.,
2000
Leaf disc/
N/A
ACC
deaminase
PRB-1b
promoter
risk assessment
promoter crop
plant
A. tumefaciens
Zaidi et al.,
2005
strain
LBA4404
potato leaf
and stem
specific
promoter
N/A
N/A
rolD (root
specific
promoter)
ST-LS1
Leaf disc/
A. tumefaciens
strain
LBA4404
PRB-1b
(pathogenesi
s related
promoter)
cry2Aa2
promoter
Selectable
marker
N/A
N/A
N/A
risk assessment
Wei et al.,
2007
28
U. of
Ottawa/
Canada
cry2Aa2
cry2Aa2/
Bacillus
thuringiensis
promoter
ST-LS1
potato leaf
and stem
specific
promoter
Selectable
marker
nptII gene
Leaf disc/
A. tumefaciens
regenerate
N/A
promoter crop
plant
strain
LBA4404
29
1. .
113
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02-564-6703
E-Mail: valy@biotec.or.th
2. .
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E-Mail: p.burns@biotec.or.th
3. .
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5. ..
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8. .
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30
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E-Mail: fscissa@ku.ac.th
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16. .
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0 2218 5386
E-Mail: jessada.d@chula.ac.th
13. .
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02-218-7533
E-Mail: patanadorn@yahoo.com
14. ..
10900
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02-579-5528
E-Mail: fscisrp@ku.ac.th
17. .
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02-579-5528
18.
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19.
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20.
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. 02-579-0113 3697
034-351-980
31
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E-Mail: scpsk@mahidol.ac.th
26. .
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043-364-169
E-Mail: sumbun@kku.ac.th
27. .
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. 085-008-7300
043-203-435
E-Mail: yupa_han@kku.ac.th
28. .
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. 053-892-259
053-892-259
E-Mail: soanun@science.cmu.ac.th
29. .
. 50202
. 053-873-535-7 111
053-878-225
E-Mail: chotipas@mju.ac.th
32
30. .
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. 054-342-553
054-342-550
E-Mail: pattamasirithunya@yahoo.com
31. ..
10520
. 02-737-3000 6262
02-326-4414
E-Mail: kskanokp@kmitl.ac.th
32.
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E-Mail: subinh@chmenthai.co.th
33. .
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113
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0 2564 6701
E-Mail: somvong.tra@biotec.or.th
34. .
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E-Mail: oraprapai@biotec.or.th
35. .
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33
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. 02-579-0113 3700
034 351908
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. 02-579-0113 3700
034 351908
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113
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E-Mail: siriporn@biotec.or.th
42.
113
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0 2564 6701
E-Mail: tanaporn@biotec.or.th
43.
. . 6
10400
. 02-644-8150 520
02-644-8109
E-Mail: watcharin@biotec.or.th
44.
. . 6
10400
. 02-644-8150 520
02-644-8109
E-Mail: surisa@biotec.or.th
45.
. . 6
10400
. 02-644-8150 520
02-644-8109
E-Mail: duangkamol@biotec.or.th
46.
113
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. 02-564-6700 3318
02-564-6703
E-Mail: chalinee@biotec.or.th
34
47.
113
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. 02-564-6700 3374
02-564-6703
E-Mail: Sathin@biotec.or.th
48.
113
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. 02-564-6700 3372
02-564-6703
E-Mail: Jintana.cha@biotec.or.th
49.
113
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02-564-6703
E-Mail: sarunya.jin@biotec.or.th
35