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  • The Light Microscope Magnification: Microscopes

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    noor
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    The document discusses different types of microscopes and their uses: 1. Light microscopes have different magnification levels depending on the eye piece and objective lens used, with oil immersion lenses providing the highest magnification of 80-100x. They are used to examine unstained samples down to a resolution of 0.25 micrometers. 2. UV microscopes use UV light and fluorescent dyes, allowing examination of samples down to 75 nanometers. 3. Dark field microscopes use a powerful lamp and dark field condenser to illuminate thin samples against a dark background, useful for examining thin unstained bacteria. 4. Electron microscopes have the highest magnifications up to 100,000x and

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    Microscopes

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    The document discusses different types of microscopes and their uses: 1. Light microscopes have different magnification levels depending on the eye piece and objective lens used, with oil immersion lenses providing the highest magnification of 80-100x. They are used to examine unstained samples down to a resolution of 0.25 micrometers. 2. UV microscopes use UV light and fluorescent dyes, allowing examination of samples down to 75 nanometers. 3. Dark field microscopes use a powerful lamp and dark field condenser to illuminate thin samples against a dark background, useful for examining thin unstained bacteria. 4. Electron microscopes have the highest magnifications up to 100,000x and

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    Attribution Non-Commercial (BY-NC)
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    8 views3 pages

    The Light Microscope Magnification: Microscopes

    Uploaded by

    noor
    The document discusses different types of microscopes and their uses: 1. Light microscopes have different magnification levels depending on the eye piece and objective lens used, with oil immersion lenses providing the highest magnification of 80-100x. They are used to examine unstained samples down to a resolution of 0.25 micrometers. 2. UV microscopes use UV light and fluorescent dyes, allowing examination of samples down to 75 nanometers. 3. Dark field microscopes use a powerful lamp and dark field condenser to illuminate thin samples against a dark background, useful for examining thin unstained bacteria. 4. Electron microscopes have the highest magnifications up to 100,000x and

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as doc, pdf, or txt
    of 3

    Microscopes

    :The

    light microscope

    : Magnification

    Mag. of eye lens 10 Mag. of low power obj. 10 Mag of high power obj. 40 Mag. of oil immersion lens (O.I.L.) 80-100 Total Mag.= Mag of eye piece Mag. of obj. lens Power of resolution = 0.25 u to use good source of light -1 .plane side of mirror -2 .move the condenser completely up -3 .diaphragm is completely opened-4 use the O.I.L -5 place a drop of cedar wood oil on the stained film- lower the .O.I.L until it touches the oil,using the coarse adjustment .Adjust the film into view using the fine adjustment violet Microscope Source of light: U.V. rays ,this gives a greater power of* (resolution (75 nanometers .Lenses are made of quartz instead of glass* U.V. rays (Invisible)+fluorescent dyes ---- visible* . ((fluorescence If serum Antibodies + fluorescent dyes------ Immunofluorescent .technique :Dark ground micro *.Powerful Lamp Dark field condenser*
    :Ultra ? How

    It is used mainly to examine very thin unstained bacteria e.g. the spirochoetes. The bacteria appear brightly illuminated in a dark .background Micro .Electron beams as a source of electrons* .Electromagnetic lenses and condenser* Electrons form an image on a fluores.screen* It is used to see the finest structure of the bacteria and the viruses as it has a magnifying power up to 100.000 times and resolution (power one nanometer (1/1000 of the micron
    :Preparation :Electron

    of film

    Prepare a clean new slide -------- flaming-1 .Place a drop of water-2 With a flamed and cooled wire loop take a small amount-3 . of bacterial culture ,mix in water .Dry-4 With the smear side upward , pass the slide three times-5 .through the flame to fix the bacteria to the slide ----- stain Stain Cover the smear with methylene blue 1 min .Wash---- leave to dry ----oil ----- examine
    :Gram Stain :Simple

    smear with crystal violet for 1 min-1 Wash with tap water-2 (cover with iodine for 1 min. (fixative-3 alcohol (decolorizing)-apply it several times until no more-4 .stain can be washed off wash with tap water-5 .cover the smear with safranin as a counter stain for 1 min-6

    .Cover the

    wash ----dry ------ O.I.L-7 G + ve Violet G -ve red

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