Dr.

Sangeetha Nagarajan

Antibiotics and Protein synthesis 1. Chloramphenicol It is bacteriostatic It binds to 50S ribosomal subunit It Inhibits peptidyl transferase in prokaryotes.

2. Puromycin Puromycin inhibits protein synthesis at translation by prematurely terminating a peptide chain.

In simple terms, the part of puromycin that resembles an aminoacyl end of tRNA can bind to the A site of a ribosome, (forming a peptide bond), but the end product will not participate in translocation to the P site. Specifically, puromycin serves as an acceptor of the peptidyl chain from peptidyl-tRNA in the P

Dr.Sangeetha Nagarajan

site, in a reaction in which peptidyl transferase catalyzes the attachment of the peptidyl chain to the free NH3+ group of puromycin. This lack of participation in translocation results in dissociation from the ribosome and early peptide termination. This early termination means the release of truncated, nonfunctional polypeptides 3. Streptomycin It is bacteriocidal It belongs to the aminoglycoside group It binds to 30S ribosomal subunit and prevents initiation It can also causes misreading by interfering with the normal pairing between codon and anticodon.