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Code No: D0302 JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD M.Tech II - Semester Examinations, October/November 2011 GENETIC ENGINEERING (BIOTECHNOLOGY) Time: 3hours Max. Marks: 60 Answer any five questions All questions carry equal marks --1. Explain the structure, function & applications of the following enzymes. a) Terminal deoxynucleotidyl transferase b) Reverse transcriptase.

R09

[6+6]

2.

Describe in detail c-DNA synthesis & the protocol for construction of c-DNA library. [12] Discuss site directed mutagenesis in detail & its application in protein engineering. [12] Write about regulation of antibody synthesis in mammals. Write short notes on: a) Nucleic acid microarray b) Northern blot.

3.

4. 5.

6.

Write in detail the salient features of recombinant DNA biosafety guidelines & risk assessment. [12] Comment on: a) Gene Knock-out technology b) Strategies of gene delivery.

7.

8.

Explain the principle & steps involved in the classical yeast two-hybrid system & its applications. [12] ********

T N

W U

R O

D L

[12]

[6+6]

[6+6]

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