DEPARTAMENTO DE BIOQUMICA Y BIOLOGA MOLECULAR

GRADO BIOTECNOLOGIA

Calificacin

NAME:
D.N.I.:

(ARA G1)
October 2012

Laboratory Session 1: PROTEINS.

PART 1. ISOLATION OF CASEIN FROM MILK.

Casein Concentration [g/l] =

Efficiency (%) =

PART 2. NINHYDRIN. Write down the results of each reaction:

Ovoalbumin __________________

Glycin ______________________

Proline _____________________

Milk _____________________

Q: Is there any tuve with a different resul? ______ Explain: ___________________________

_______________________________________________________________________

PART 3. PROTEIN SOLUBILITY. Write down the results of each tube.

HCl _______________________

NaOH _______________________

Ethanol _______________________

100C_______________________

PART 4. BIURET ASSAY.

Note on the following table the concentrations (X axis) and the absorbance (Y axis) .
CONC

ABS

2
3
4
5
6
A
B
Final Result = [

]mg/ml

Plot the standard curve and the values of the experimental samples A and B: