Zato?

Sigurnost na radu u laboratoriju

Zatita za:
vas kolege pomono osoblje posjetioce posao

Prije rada, treba poznavati

Svojstva kemikalija i reagenasa Koritenje opreme i instrumenata to uiniti kod prolijevanja (adsorbensi, dezinfekcija) Kuda pobjei Gdje je aparat za gaenje poara i prva pomo

Najea ozljeda: posjekotina Statistika se odnosi na istraivake laboratorije

Opasne tvari
Fizika opasnost elektrina mehanika temperatura elektromagnetsko zraenje Bioloka opasnost

Radioaktivnost

Komprimirani plinovi Zapaljive tvari Oksidansi Toksine tvari Korozivne tvari Reaktivne tvari

Ope pravilo za opasne tvari

Proitaj etiketu tri puta
Kad uzima iz skladita Prije otvaranja Prije koritenja

Komprimirani plinovi

Oznai datum otvaranja!!!!

Zapaljive tvari

LEDROIT 22 prosinca, 1997

Zapaljive tvari
Spremati u za to predviene spremnike Ne zajedno s oksidansima

Sve boce oznaiti!!!

A da nisu bile u tom spremniku..

Korozivne tvari
Storage
Posebni spremnici za korozivne tvari Ne kiseline i baze skupa Ne koncentrirana kiselina ispod slivnika

OZNAAVANJE!!!!

Rad s korozivnim tvarima

VUK ne!

Reaktivne tvari
Pogledaj kako izgledaju!

+ +

Inkompatibilne kemikalije

Kriogene tekuine
Vrelite -73C e.g., teku i duik, helij ili argon, suhi led Mala koliina tekuine stvara veliku koliinu plina! Opekotine

Suhi led
opekotine guenje

Rizici povezani sa strujom

Mehanika opasnost

Centrifuge

Carleton University, 1999

Otvaranje, lom epruvete, prolijevanje

Autoklavi

Glassware

Control Measures

Inspect glassware for cracks, stars, or stress lines before use Repair or discard defective glassware Wear eye protection when working with glass apparatus Wear safety gloves when inserting glass into rubber or plastic tubing Do not wash broken glassware with other glassware Do not mix glassware or broken glass with regular garbage. Dispose in cardboard boxes or designated containers for recycling

Eye protection Gloves Lab coats Footwear Fume hoods Emergency showers Eyewashes Fire extinguishers

Working Alone / After Hours

Never work alone in high risk area Use buddy system Phone Protection Services Have friend contact lab at regular intervals

Eye Protection

Use appropriate safety glasses Avoid use of contact lenses? Goggles for liquids Safety glasses for explosion risks

Wear at all times in labs

Gloves

Lab Coats
Protects clothing Protects body Should have snaps Non-flammable fabric Do not wash with regular clothing

Minimizes contact with hazardous agent Use appropriate gloves PVC, latex, rubber, nitrile, polypropylene Check resistance chart Do not wear gloves in halls or elevators

http://www.science.uottawa.ca/HS/glove_main.htm

Footwear

Fume Hoods

Closed-toed shoes must be worn in labs Sandals are never to be worn in labs Shoes must cover the foot Steel toed boots must be worn in mechanical areas

Chemical hoods Biosafety hoods Radioisotope hoods Exhaust canopies Recirculating hoods Perchloric acid hoods Hydrofluoric acid hoods

Fume Hood Design

Sash Bypass Grills Face Velocity Baffle Exhaust duct Face opening HEPA filter Carbon filter

Fume Hood Safety

Never put your head into a fume hood Minimize storage of chemicals or equipment in a fume hood Work with sash at indicated operating level Set up apparatus or sources of emission at least six inches behind the plane of fume hood Raise large pieces of equipment at least one inch above the floor of the fume hood Do not obstruct back slots Keep sash closed when not working in fume hood Avoid cross drafts when working in a fume hood

Emergency Showers/ Eyewashes

Emergency Showers/ Eyewashes

Know location of emergency showers and eyewash stations Become familiar with operation Wash for minimum of 15 minutes with running water Remove all contaminated clothing Wait for medical assistance Report incident

Fire Extinguishers

Emergency Procedures
Fires Explosions Spills Emissions First aid Injuries Accidents Incident/accident report form Property damage 5411

Attend faculty training Extinguish fire only if feel comfortable ABC for regular fires D for metal induced fires Pull fire alarm

Emergency Procedures
Contact Protection Services for emergencies at extension 5411 If safe to clean spill by yourself, proceed After incident or accident, complete incident/ accident form Investigation by EHSS, Human Resources and Occupational Health and Safety Committee

Waste Disposal
Regulated by Ontario University procedures Faculty guidelines Sewer restrictions Air restrictions Chemical waste Biomedical waste Sharps waste Battery waste Oil waste Scintillation wastes Photographic waste Regular garbage

Waste Disposal

Waste Disposal - General

Guidelines
Use approved containers for disposal, (see EHSO) Label all hazardous waste containers with University hazardous waste label Do not mix incompatible wastes Do not overfill waste containers Contact EHSS for disposal of unstable reactive chemicals, e.g., dry picric acid Contact faculty representatives for disposal

Label all wastes University Hazardous Waste label Sharps containers Solvent waste containers Broken glass in boxes Batteries recycling

Before Leaving the Lab

Check Overnight operations
Supplies are sufficient Waterlines are adequately clamped Description of the process posted Emergency contacts posted

Overnight incident
Thermometer melted from heat Mercury vaporized Rubber supports melted Fire burnt itself out

Before Leaving the Lab

Turn off
Gas Water Power lines Other non necessary equipment

Weekly Lab Checks

Purge Eyewash station Fire extinguisher First Aid Kit Fume Hood Tubing, pressurized connection Chemical storage Cap Sinks, floor drains
Page 5 - Laboratory Procedures and Safety Manual

Clean your work area Return ALL chemicals to storage Lower fume hood sash Wash your hands

Printed Resources

Find the potentials

Whats Wrong? #1

Faculty Laboratory Procedures and Safety Manual WHMIS Manual University Hazardous Materials Handling Guide CRC Handbook of Laboratory Safety Prudent Practices in the Laboratory The Chemical Institute of Canada Lab Safety Handbook

Find the potentials

Whats Wrong? #2

Find the potentials

Whats Wrong? #3

Find the potentials

Whats Wrong? #4

Find the potentials

Whats Wrong? #5

Accident waiting to happen!

Did this person take Lab Safety???

Opi rizici u laboratoriju

Vatra Staklo Otri predmeti Prolijevanja Plinske boce Kemijski rizik Bioloki rizik Radijacija

Glavne skupine

Regencije i reagensi
Proitaj etiketu!

Reagencije i reagensi Infektivne tvari Radijacija Sigurnost prostorija

Zatitna odjea i eventualna dodatna oprema Znati to se radi u sluaju nezgode

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Oznake na kemikalijama
Proizvoa je duan napisati sve na etiketu
Ime Rizici i opasnosti Mjere predostronosti Prva pomo Rukovanje i pohrana

Zdravstveni rizici
Karcinogenost Toksinost Visoka toksinost Reprodukcijski rizik Iritacija

Hepatotoksin Nefrotoksin Neurotoksin

Tipine mjere predostronosti

Ne udisati pare Koristiti u prostoriji koja ima prozraivanje - digestor Nakon upotrebe zatvoriti spremnik Izbjei kontakt s koom

Eksplozivne/zapaljive kemikalije
Ne dovesti u dodir s iskrenjem, toplinom i plamenom Ne dovesti u dodir sa zapaljivim materijalima

Etikete
Opasnost Upozorenje Oprez

Toksinost Korozivnost (kiseline i baze) Zapaljivost (zapaljiva otapala i neke krutine) Reaktivnost (natrij i razliiti reagensi koji reagiraju s vodom)

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Putevi ulaska
Inhalacija Ingestija Apsorpcija kroz kou ili sluznice (Injekcija)
Akutno trovanje (npr. CO) Kronino trovanje (npr. Pb) Kumulativno trovanje (npr. teki metali) Sinergistiki efekt (kombinacija npr. alkohol i klorirani ugljikovodici)

A. Benedi Hitna transplantacija jetre uslijed akutnog zatajenja

Kako se zatititi?

Interakcija alkohol -acetaminofen

Digestor

Osobna zatita

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Osobna zatita
Kute Kosa Obua
Skinuti rukavice prije: rada na aparatima telefoniranja, izlaska iz laboratorija

Vatra
Zapaljive tvari
Koristiti minimalnu koliinu Pohraniti u specijalne ormare Zagrijavanja provoditi kontrolirano
(npr. vodena kupelj, a ne plamenik)

Stakleno posue
Treba znati ta uiniti u sluaju vatre Trening svog osoblja!
Odlaganje slomljenog posua

13

Prolijevanje
Odmah oistiti Odloiti u toksini otpad ukoliko se radilo o potencijalno opasnoj kemikaliji

Plinske boce
Po mogunosti smjestiti izvan laboratorija Uvrstiti za pozadinu Redovito kontrolirati regulatore

Urednost
Uredan laboratorij manja ansa za nezgodu, a vea za uspjean posao

Laboratory Equipment
Ne koristiti opremu za koju niste uvjebani, s obzirom da to nosi rizik ozljede i materijalne tete

Prva pomo
Za sve kemikalije isprati s puno vode, 10 minuta

Otpad
Bioloki Kemijski

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Ako ne zna, naprije pitaj!!!

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