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    Bio Chem

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    Ian Dilao
    A series of chemical tests were performed on samples of lipids to identify their properties. Acrolein testing found characteristic odors for glycerol, coconut oil, and lecithin. Unsaturation testing measured the number of drops different oils resisted flowing, indicating olive oil and oleic acid were the most unsaturated. Lecithin tested positive for phosphate with a yellow precipitate. Emulsification tests showed layer separation of coconut oil and bile salts but mixing of olive oil and lecithin. Liebermann-Burchard and Carr-Price reactions identified colors for cholesterol, bile salts, and various oils. A modified Furter-Meyer test produced a slight yellow color for α-tocopherol

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    Bio Chem

    Uploaded by

    Ian Dilao
    131 views2 pages
    A series of chemical tests were performed on samples of lipids to identify their properties. Acrolein testing found characteristic odors for glycerol, coconut oil, and lecithin. Unsaturation testing measured the number of drops different oils resisted flowing, indicating olive oil and oleic acid were the most unsaturated. Lecithin tested positive for phosphate with a yellow precipitate. Emulsification tests showed layer separation of coconut oil and bile salts but mixing of olive oil and lecithin. Liebermann-Burchard and Carr-Price reactions identified colors for cholesterol, bile salts, and various oils. A modified Furter-Meyer test produced a slight yellow color for α-tocopherol

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    BioChem

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    A series of chemical tests were performed on samples of lipids to identify their properties. Acrolein testing found characteristic odors for glycerol, coconut oil, and lecithin. Unsaturation testing measured the number of drops different oils resisted flowing, indicating olive oil and oleic acid were the most unsaturated. Lecithin tested positive for phosphate with a yellow precipitate. Emulsification tests showed layer separation of coconut oil and bile salts but mixing of olive oil and lecithin. Liebermann-Burchard and Carr-Price reactions identified colors for cholesterol, bile salts, and various oils. A modified Furter-Meyer test produced a slight yellow color for α-tocopherol

    Copyright:

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    Download as docx, pdf, or txt
    131 views2 pages

    Bio Chem

    Uploaded by

    Ian Dilao
    A series of chemical tests were performed on samples of lipids to identify their properties. Acrolein testing found characteristic odors for glycerol, coconut oil, and lecithin. Unsaturation testing measured the number of drops different oils resisted flowing, indicating olive oil and oleic acid were the most unsaturated. Lecithin tested positive for phosphate with a yellow precipitate. Emulsification tests showed layer separation of coconut oil and bile salts but mixing of olive oil and lecithin. Liebermann-Burchard and Carr-Price reactions identified colors for cholesterol, bile salts, and various oils. A modified Furter-Meyer test produced a slight yellow color for α-tocopherol

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    of 2

    Results

    A. Acrolein test Sample Glycerol Coconut oil Lechitin Olic Acid Results Rubbery odor Candle wax odor Rubbery odor Burned fat odor

    B. Test for unsaturation Samples Olive oil

    Results/Description 146 drops

    Oleic acid

    139 drops

    Stearic acid

    8 drops

    Coconut oil

    15 drops

    C. Test for phosphate Sample Lechitin

    Results/Description Yellow ppt.

    D. Emulsification test Mixtures 1mL coconut oil + 5 drops .1% bile salt solution

    Description 2 layers were formed. Upper layer was light yellow, lower was dark yellow

    Sketch

    1mLcoconut oil + 5 drops water + tiny crystal of cholesterol

    Clear liquid with yellow/white ppt.

    1mL olive oil + 5 drops 1% aqueous solution of lechitin

    Clear liquid with white ppt.

    E. Leiberman-Burchard reaction Samples Cholesterol Bile salts

    Result Dark blue-green. Reddish/maroon

    F. Carr-Price reaction Samples Cod liver oil

    Description Blue solution Opaque solution

    Coconut oil Opaque solution Olive oil

    G. Modified Furter-Meyer test Samples -tocopherol

    Description Slight yellow color

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