Professional Documents
Culture Documents
Large-Scale Production of Recombinant Proteins
Large-Scale Production of Recombinant Proteins
Lecture 6
ISAT 451, Fall 2003 College of Integrated Science and Technology James Madison University
Most commercially valuable proteins (industrial enzymes or therapeutic proteins) require living cells to make the product Although cells can be readily grown in a standard 200 ml laboratory flask, commercial production may require containers in excess of 10,000 liters What are the limitations of large-scale production?
Sterile culture medium containing a carbon source and essential salts or an enriched medium Pure culture for inoculation oxygenation and mixing Regulation of temperature Regulation of pH
Batch Fermentation
Sterile medium is inoculated and fermentation proceeds without the addition of fresh growth medium.
Fed Batch
Nutrients are added at intervals during fermentation. No growth medium is removed.
Continuous Fermentation
Fresh growth medium is added continuously during fermentation and spent medium is removed.