REAGENT LIST

Reagents in black will not be provided by the hESC SRF except during the in house training period Reagents in red, need to be tested and/or expensive and will be provided by the hESC SRF
INDEX 1 2 3 4 5 6 7 8 9 Product TUBES Nunc conical 200ml centrifuge tubes 14 ml tube 15 ml tube 50ml tubes 5ml round-bottom tube 5ml tube w/ cell strainer 5ml Vantage tubes PLATES 6 well plate 12 well plate 24 well plate 48 well plate BD/Falcon BD/Falcon BD/Falcon BD/Falcon 50/cs 50/cs 50/cs 50/cs 353046 353043 353047 353078 48 53 53 126.88 Nunc BD/Falcon BD/Falcon Crystalgen BD/Falcon BD/Falcon BD/Falcon 48 500/cs 500/cs 500 500/cs 500/cs 1000/cs 376813 352059 352099 L-2263p 352063 352235 352008 87 109 79.00 105.00 109 407.38 34.00 PLASTIC WARE MATRICES REDUCING AGENTS DISSOCIATION REAGENTS MEDIA FREEZING/FIXING MEDIA SERUMS ANTIBODIES CYTOKINES Company Size Catalog # PAGE # 1 3 3 3 3 4 4 5 7 Price $

96 well plate(flat-bottom) 96 well plate(V-bottom) 96 well plate(u-bottom) 6 well, low cluster plate 24 well low cluster 96 well low cluster DISHES 35X10 mm Petri dish 100 X 20 TC DISH 150X25mm GRID 100X15mm Petri Petri 60X15mm NUNC 240835 (245mm) SCRAPER/FILTERS Cell Scraper, 25cm Corning 1000ml filter 0.22mm Corning 500ml filter 0.22mm Corning 250ml filter 0.22mm Corning 150ml filter 0.22mm 50ml Steriflip 022mm 50ml cellstrainer 70micr Corning Costar .22 syringe filter Corning Costar .45 syringe filter BOTTLES 125ml bottles 250ml bottles 500ml bottles 1000ml bottles

BD/Falcon Costar BD/Falcon Costar Costar Costar

50/cs 50/cs 50/cs 24/cs 24/crate 24/crate

353072 3894 353077 3471 3473 3474

63 240.61 74 187 194 276

BD/Falcon BD/Falcon BD/Falcon BD/Falcon BD/Falcon Nunc

500 353003 353025 351029 353002 240835

351008 200/CS 100/CS 500/CS 500/CS 16/cs

163 74 89 73 80 105

Sarstedt Corning Corning Corning Corning PK (FScientific) Fisher Scientific Costar Costar

100 12/crate 12/crate 12/crate 12/crate 25/pack 50/pack 50/cs 50/cs

83-1830 430186 431097 430756 431153 SCGP00525 087712 07-200-370 07-200-371

182 141.52 78 60 52 72.00 66.00 91 91

Costar Costar Costar Costar

24/crate 12/crate 12/crate 12/crate

8388 8390 8393 8396

51 33 38 53

PIPETTES 1ml pipette 2ml pipette 5ml pipette 10ml pipette 25ml pipette 50ml pipette 1ml syringe 3ml syringe 5ml syringe 10ml syringe 1.8ml cryovials 1.5ml microtubes 1-200uL tip reloads 101-1250uL tip reloads Pasteur pipette 20-gauge needles 16-gauge needles Tripleflask Greiner T-25 Greiner-T75 Pipetting reservoir cryo-inserts (white) cryo-inserts (yellow) cryo-inserts (orange) cryo-inserts (pink) cryovial inserts (grey) cryo-inserts (purple) cryo-inserts (blue) cryo-inserts (red) cryo-inserts (green) cryo-inserts (brown) Super frost slides BD/Falcon BD/Falcon BD/Falcon BD/Falcon BD/Falcon BD/Falcon BD/Falcon BD/Falcon BD/Falcon BD/Falcon Nunc Denville VWR Monoject Monoject Nunc Greiner Greiner VWR Nunc Nunc Nunc Nunc Nunc Nunc Nunc Nunc Nunc Nunc Fisherbrand 1000 1000 200 200 200 100 100 100 100 100X4 450X4 960 1000 500/cs 1000/cs 32 200/cs 160/cs 50 500/PK 500/PK 500/PK 500/PK 500/PK 500/PK 500/PK 500/PK 500/PK 500/PK 144 357520 357507 357543 357551 357525 357550 309602 309585 309603 309604 375418 c-3302-1 P2201 1112-2700 14673-043 22-256-569 05-561-51 12-565-24 T3002-1 T3002-3 20901-921 354755 355077 355158 375884 375906 375922 354879 354968 355018 375868 12-550-15 99.61 109.71 29.00 32.00 89 119 6.62 5.05 9.65 42 652 11.75 22.50 20.71 35.94 139 277 266 86.00 99.00 70 25 25 25 25 25 25 25 25 31.94 25 35

ISC Scientific EACH USA Scientific 96X10

MATRICES Gelatin (Prepared to 0.1%) Matrigel Fibronectin Laminin Sigma BD BD Biosciences BD Biosciences 500g 10ml 5mg 1mg G1890 356230 356008 354232 38.6 205 252.00 97.00

REDUCING AGENTS -Mercaptoethanol Ascorbic Acid Monothioglycerol DISSOCIATION REAGENTS Collagenase B Collagenase from C.histolyticum Trypsin-EDTA (0.25%) DNAse I Bovine Pancreas Dispase (1 mg/mL) in DMEM/F-12 MEDIA Distilled water 1X PBS 0.1% Gelatin CWFS DMEM/F12 DMEM DMEM powder IMDM IMDM powder Cellgro 500ml/6cs 25-055-CV 21-031-CM 32 64 Mediatech/Cel 1L, 6/cs lgro Sigma Cellgro 500gms 500ml, 6/pk Roche Sigma Invitrogen VWR 2.5g 5gms 100ml 10MU 1088831 C0130-5G 25-053-CL 80510-412 07923 397 1155 28.8 147 31 Sigma Sigma Sigma 25ml 25g 100ml M3148 A4544-25G M6145 9.95 17 25

Stem Cell 100ml Technologies

G9391-500G 61.00 10-092-cv 15-018-CV 12100-046 MT-15-016CV 12200-069 53 57 37 45 (VWR) 77

Fisher Scien 500ml, 6/cs Invitrogen 10X1L Mediatech/Cel 500ml, lgro 6/cs Invitrogen 10X1L

HEPES Stempro 34 SFM, combo Stempro34 supplement Neurobasal Media- SFM Hams F12 media Mef CM L-Glutamine Penicillin/Streptomycin Transferrin N2 supplement B27 Supplement Non-Essentail Amino Acids Methylcellulose Eosin Y Vinblastine sulfate salt FREEZING /FIX/Stain DMSO PFA (16%)

Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Homemade Invitrogen Invitrogen Roche Invitrogen Invitrogen Invitrogen Fluka Fisher Sigma

100ml 500ml 500ml 500ml 500 ml /// 100ml 100ml 20ml 5ml 10ml 25ml 500g 25gm 5mg

15630-080 10639-11 10639-11 21103049 10-080-CV /// 25030-081 15070-063 652-202 17502048 17504044 11140-050 64630 E-511 V1377

47 120 120 38 38 /// 21 15 297.00 40 60 14.5 51.15 28 41.67

Sigma Electron Microscopy Sc. Sigma Sigma Sigma

100ml 10ml

D5879 15710

18.7 20.00

25% Glutaraldehyde Giemsa May-Grunwalds

50ml 500ml 500ml

G5882 GS-500 MG-500

81.60 38.90 41.00

SERUM/SR/BLOCK SER Gemcell Wash serum T4 serum T6 serum Gemini BioProducts Fetalplex Atlas Biologicals Atlas Biologicals 500ml 500ml 500mls 500mls 100-525 100-106 F-0500-D F-0500-D 245 99 190 190

PDS PFHM-II KO Serum Replacement Normal Donkey serum Normal Human serum Normal Chicken serum Normal Rat serum Normal Goat serum BSA Faction V 7.5% solution MEFs p0

Animal 1L Technologies Invitrogen Invitrogen 1L 500ml

Order according to Keller Protocol

560 41.62 193.55 33.00 35.00 24.00 23 45 28

12040-077 10828028 017-000-121 009-000-121 003-000-120 012-000-120 S26/100ml 15260-037

Jackson Imm 10ml Res. Jackson Imm 10ml Res. Jackson Imm 5ml Res. Jackson Imm 10ml Res. Chemicon Intl 10ml Invitrogen Homemade 100ml

Please note that only some of the prices below reflect the 23% discount on antibodies from Invitrogen and the 20% discount of R&D antibodies. Antibodies Company R&D systems Caltag BD R&D systems BD Caltag BD BD Invitrogen BD BD Caltag R&D systems Catalog # FAB357A LCD11705 559777 FAB1448A 555369 MHCD0812 555342 555976 MHCD4504-4 550956 551061 LCD34-581-05 FAB1435A Price 228.75 220 288 275 310 220 310 390 580 320 310 220 275

APC's
KDR-APC CD-117 CD-41A Alkaline phosphatase CD-8 CD-8 PECY7 CD-3 CD-184 CD-45 CD-36 CD-42B CD-34 SSEA-4

CD-235A CD-11B

BD Caltag

551336 LCD11B05

310 220

PE's CD-41A CD-42B CXCR-4 CD-19 CD-34 PEcy5 CD-117 CD-31 Brachyury CD-49B CD-71 CD-4 PODXL CD-56 KDR-PE CD-110 CD51/61 CD-38 CD-25 FITC CD-43 CD-19 CD-45 CD-31 CD-26 CD-3 CD-71 BIO CD-184 CD-31 BD BD 555973 553371 265 305 7 BD BD Caltag BD BD BD Caltag 555475 555412 MHCD4501 555445 FAB1180F 555339 MHCD7101 230 230 183 216 220 230 183 Caltag BD Caltag Caltag BD BD BD R&D systems BD Caltag BD R&D systems Caltag R&D systems BD BD Caltag BD MHCD4104 555473 MHCXCR404 LMHCD1917 555823 340867 555446 IC2085P 555669 MHCD7104 555347 FAB1658P LMHCD5604 FAB357P 551313 550037 MHCD3804-4 555432 216 243 276 250 295 204 260 280 ---216 260 240 175 216 ----270 625 260

anti-hNKX2 Misc. Cadherin-5 CD31 (IHC) SOX17 CD-4 Cadherin (hN) Cadherin(hE) Oct-4

R&D systems BD Caltag R&D systems Caltag R&D systems R&D systems Biovision

BAF2444 555661 550389 BAF1924 LMHCD0400 MAB1388 BAM18381 3576-100

300 205 ---342 102 200 270 245

CYTOKINE (Cat #) HUMAN CYTOKINES Endothelial cell growth supplement (ECGS) Recombinant human /mouse/rat Activin A Recombinant human Activin B Recombinant human BMP4 Recombinant human BMPR-1A/ALK-3/Fc Chimera Recombinant human BMPR-1B/ALK-6/Fc Chimera Recombinant human Dkk1 Recombinant human EGF Recombinant human EPO Recombinant human FGF2 Recombinant human FGF4 Recombinant human G-CSF

Company BD R&D R&D R&D R&D R&D R&D R&D Amgen R&D R&D R&D

Cat # 356006 338-AC 659-AB 314-BP 315-BR 315-BR 1096-DK 236-EG Sinai 233-FB 235-F4 214-CS

Stock NO YES NO YES YES YES YES YES YES YES NO YES

/vial 100mg 250 ug 250 ug 250 ug 100 ug 100 ug 250 ug 250 ug 10,000U 250 ug 25 ug 50 ug

Recombinant human GM-CSF Recombinant human HGF (R&D Recombinant human IGF-1 Recombinant human IGF-II Recombinant human IL-3 Recombinant human IL-6 Recombinant human M-CSF Recombinant human Netrin Recombinant human Osteopontin Recombinant human SCF Recombinant human Sonic Hedgehog Recombinant human TPO Recombinant human VEGF MOUSE CYTOKINES Recombinant mouse DLL4 Recombinant mouse EGF Recombinant mouse GM-CSF Recombinant mouse IGF-I Recombinant mouse IL-11 Recombinant mouse LIF Recombinant mouse M-CSF Recombinant mouse Sonic Hedgehog Recombinant mouse TPO Recombinant mouse Wnt 3A Recombinant mouse Wnt 5A

R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D R&D

215-GM 294-HG 291-G1 292-G2 203-IL 206-IL 216-MC 1254-N4 1433-OP 255-SC 1845-SH 288-TP 293-VE 1389-D4 2028-EG 415-ML 791-MG 418-ML 449-L 416-ML 461-SH 488-TO 1324-WN 645-WN

YES YES YES YES YES YES NO NO NO YES NO YES YES NO NO NO NO NO No NO NO NO YES YES

50 ug 250 ug 250 ug 25 ug 250 ug 50 ug 25 ug 25 ug 50 ug 50 ug 50 ug 250 ug 50 ug 50 ug 200 ug 25 ug 50 ug 25 ug 50 ug 50 ug 25 ug 50 ug 200 ug 100 ug