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  • Conventional Processing Schedule, 1mm Biopsy. 2.5 HRS.: Table 2

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    Nazaqat Farooq
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    The document outlines three different processing schedules for preparing biopsy samples: a conventional schedule for 1mm biopsies taking 2.5 hours, a microwave schedule for 1mm biopsies taking around 45 minutes, and a conventional schedule for 3mm biopsies taking 12 hours. The schedules involve steps of dehydration using increasing alcohol concentrations or other agents, clearing with xylene, and infiltration with paraffin wax.

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    histology

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    Processing Schedule

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    The document outlines three different processing schedules for preparing biopsy samples: a conventional schedule for 1mm biopsies taking 2.5 hours, a microwave schedule for 1mm biopsies taking around 45 minutes, and a conventional schedule for 3mm biopsies taking 12 hours. The schedules involve steps of dehydration using increasing alcohol concentrations or other agents, clearing with xylene, and infiltration with paraffin wax.

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    Download as DOCX, PDF, TXT or read online from Scribd
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    Download as docx, pdf, or txt
    5 views1 page

    Conventional Processing Schedule, 1mm Biopsy. 2.5 HRS.: Table 2

    Uploaded by

    Nazaqat Farooq
    The document outlines three different processing schedules for preparing biopsy samples: a conventional schedule for 1mm biopsies taking 2.5 hours, a microwave schedule for 1mm biopsies taking around 45 minutes, and a conventional schedule for 3mm biopsies taking 12 hours. The schedules involve steps of dehydration using increasing alcohol concentrations or other agents, clearing with xylene, and infiltration with paraffin wax.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    Download as docx, pdf, or txt
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    Conventional processing schedule, 1mm biopsy. 2.5 hrs.

    Dehydrate Alcohol, 65% 15 min Dehydrate Alcohol, 95% 15 min Dehydrate Alcohol, 95% 15 min Dehydrate Alcohol, absolute 15 min Dehydrate Alcohol, absolute 15 min Clearing agent Xylene 15 min Clearing agent Xylene 15 min Infiltrate Paraffin 15 min Infiltrate Paraffin 15 min Infiltrate Paraffin 15 min c) Microwave processing schedule, 1mm biopsy. ~45 min. Dehydrate Ethyl alcohol, 100% --- fill DRAIN Dehydrate Ethyl alcohol, 100% 5 min 67 C, microwave DRAIN Dehydrate Isopropyl alcohol, 100% 3 min 74 C microwave DRAIN Infiltrate Liquid paraffin, 60 C fill DRAIN* Infiltrate Liquid paraffin, 60 C 2 min 65 C microwave, agitate --- 5 min 85 C microwave DRAIN* a) Conventional, room temperature processing schedule, 3mm biopsy. 12 hrs. Fixation Formalin, 10% 120 min --Fixation Alcoholic formalin 60 min --Fixation Alcoholic formalin 60 min --Dehydrate Alcohol, 95% 60 min vacuum Dehydrate Alcohol, 95% 45 min --Dehydrate Alcohol, absolute 45 min vacuum Dehydrate Alcohol, absolute 60 min --Clearing agent Xylene 60 min --Clearing agent Xylene 60 min vacuum Infiltrate Paraffin 30 min --Infiltrate Paraffin 60 min --Infiltrate Paraffin 90 min vacuum Table 2.

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