Biology 224: Lac Operon Simulation

Component
SUBSTRATES Glucose Lactose Allolactose GENE ALLELIC STATES Genomic Copies lacI lacZ lacY lacA F' Copies lacI lacZ lacY lacA

Actual Possible States State

absent, present absent, present absent, present absent present present

+, -, S +, +, +, -

+ + -

+, -, S +, +, +, -

S + +

CIS-ELEMENT ALLELIC STATES Genomic Copies operator +, C promoter +, F' Copies operator promoter CIS ELEMENTS STATES Genomic Copies CAP site operator F' Copies CAP site operator PROTEIN STATES Repressor CAP Superrepressor

C +

+, C +, -

bound, free bound, free

bound free

bound, free bound, free

bound bound

active, inactive active, inactive active, inactive

inactive active active

GENE PRODUCT SYNTHESIS Genomic Copies lacI The lac repressor +, Super repressor +, lacZ galactosidase none, leaky, low, high

+ high

lacy lac permease lacA transacetylase F' Copies lacI The lac repressor Super repressor lacZ galactosidase lacy lac permease lacA transacetylase Net (Either Copy) lacI The lac repressor Super repressor lacZ galactosidase lacy lac permease lacA transacetylase

none, leaky, low, high none, leaky, low, high

none none

+, +, none, leaky, low, high none, leaky, low, high none, leaky, low, high

+ none leaky leaky

+, +, none, leaky, low, high none, leaky, low, high none, leaky, low, high

+ + high leaky leaky

Interactive Graphics
Glucose Lactose 10 Present B galactosidase Permease Transacetylase 0 10 10 1 1

Absent

Glucose

Lactose

High

10 9 8 7 6

Low

5 4 3 2

Leaky None

1 0

B galactosidase

Permease

Transacetylase

Stress Test
Test 1 Test 2 Test 3 Test 4 Test 5 Test 6 Test 7 Test 8 Test 9 Test 10 Test 11 Test 12 Test 13 Test 14 Test 15 pass pass pass pass pass pass pass pass pass pass pass pass pass pass pass

This worksheet is used to define the possible values for cells in column C

presence present absent

wildtype + -

repressor operator + + C S

binding bound free