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    Data Pengamatan Bioklin 1

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    Riska Febriyeni Siregar
    The document reports data from an experiment measuring the absorbance of potassium permanganate (KMnO4) solutions using different pipettes and dilutions. It provides tables showing the absorbance measurements for four dilutions made using glass and piston pipettes, and calculates the mean, standard deviation, and coefficient of variation for each. Graphs plot the mean absorbance against trials for each dilution and pipette type, showing lines for the mean plus the standard deviations. The document analyses the precision and accuracy of the different pipettes and concentrations used in the experiment.

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    Data Pengamatan Bioklin 1

    Uploaded by

    Riska Febriyeni Siregar
    17 views8 pages
    The document reports data from an experiment measuring the absorbance of potassium permanganate (KMnO4) solutions using different pipettes and dilutions. It provides tables showing the absorbance measurements for four dilutions made using glass and piston pipettes, and calculates the mean, standard deviation, and coefficient of variation for each. Graphs plot the mean absorbance against trials for each dilution and pipette type, showing lines for the mean plus the standard deviations. The document analyses the precision and accuracy of the different pipettes and concentrations used in the experiment.

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    The document reports data from an experiment measuring the absorbance of potassium permanganate (KMnO4) solutions using different pipettes and dilutions. It provides tables showing the absorbance measurements for four dilutions made using glass and piston pipettes, and calculates the mean, standard deviation, and coefficient of variation for each. Graphs plot the mean absorbance against trials for each dilution and pipette type, showing lines for the mean plus the standard deviations. The document analyses the precision and accuracy of the different pipettes and concentrations used in the experiment.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    17 views8 pages

    Data Pengamatan Bioklin 1

    Uploaded by

    Riska Febriyeni Siregar
    The document reports data from an experiment measuring the absorbance of potassium permanganate (KMnO4) solutions using different pipettes and dilutions. It provides tables showing the absorbance measurements for four dilutions made using glass and piston pipettes, and calculates the mean, standard deviation, and coefficient of variation for each. Graphs plot the mean absorbance against trials for each dilution and pipette type, showing lines for the mean plus the standard deviations. The document analyses the precision and accuracy of the different pipettes and concentrations used in the experiment.

    Copyright:

    Attribution Non-Commercial (BY-NC)
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    of 8

    Data Pengamatan

    Pengenceran
    Pipet
    I
    Gelas
    I
    Piston
    II
    Gelas
    II
    Piston
    III
    Gelas
    III
    Piston
    IV
    Gelas
    IV
    Piston

    Absorbansi
    2

    0,348

    0,358

    0,323

    Jumlah

    Ratarata

    SD

    KV

    0,348

    1,054

    0,351

    0,0057

    1,628

    0,294

    0,279

    0,896

    0,299

    0,0223

    7,458

    0,512

    0,512

    0,508

    1,552

    0,511

    0,0092

    1,800

    0,468

    0,472

    0,445

    1,385

    0,462

    0,015

    3,247

    0,655

    0,685

    0,656

    1,996

    0,665

    0,017

    2,562

    0,723

    0,781

    0,761

    2,265

    0,755

    0,029

    3,902

    0,807

    0,792

    0,832

    2,431

    0,810

    0,020

    2,469

    0,811

    0,813

    0,832

    2,456

    0,819

    0,012

    1,465

    Perhitungan
    a. Perhitungan Pengenceran
    -

    Pengenceran I : 200 L 0,2 ml KMnO4


    add 1000 L 1 ml - 0,2 ml = 0,8 ml aquadest

    Pengenceran II : 300 L 0,3 ml KMnO4


    add 1000 L 1 ml - 0,3 ml = 0,7 ml aquadest

    Pengenceran III : 400 L 0,4 ml KMnO4


    add 1000 L 1 ml - 0,4 ml = 0,6 ml aquadest

    Pengenceran IV : 500 l 0,5 ml KMnO4


    add 1000 L 1 ml 0,5 ml = 0,5 ml aquadest

    b. Pengenceran I
    Pipet Gelas
    x = 0,348+0,358+0,348 = 0,351
    3

    SD =

    = 0,0057

    KV = SD . 100 = 0,0057 . 100 = 1,628 %


    x
    0,351

    Pipet Piston
    x = 0,323+0,294+0,279 = 0,299
    3
    SD =

    = 0,0223

    KV = SD . 100 = 0,0223 . 100 = 7,458 %


    x
    0,299
    c. Pengenceran II
    Pipet Gelas
    x = 0,512+0,512+0,508 = 0,511
    3
    SD =

    = 0,0092

    KV = SD . 100 = 0,0092 . 100 = 1,800 %


    x
    0,511

    Pipet Piston
    x = 0,468+0,472+0,445 = 0,462
    3
    SD =

    = 0,015

    KV = SD . 100 = 0,015 . 100 = 3,247 %


    x
    0,462

    d. Pengenceran III
    Pipet Gelas
    x = 0,655+0,685+0,656 = 0,665
    3
    SD =

    = 0,017

    KV = SD . 100 = 0,017 . 100 = 2,562 %


    x
    0,665

    Pipet Piston
    x = 0,723+0,781+0,761 = 0,755
    3
    SD =

    = 0,029

    KV = SD . 100 = 0,012 . 100 = 3,902 %


    x
    0,819
    e. Pengenceran IV
    Pipet Gelas
    x = 0,807+0,792+0,832 = 0,810
    3
    SD =

    = 0,020

    KV = SD . 100 = 0,02 . 100 = 2,469 %


    x
    0,81

    Pipet Piston
    x = 0,811+0,813+0,832 = 0,819
    3

    SD =

    = 0,012

    KV = SD . 100 = 0,012 . 100 = 1,469 %


    x
    0,819

    Grafik
    Pipet Gelas
    Absorbansi Sampel Pengenceran I
    (200 L KMnO4/1000 L Aquadest)
    0.37
    Y-Values

    Absorbansi

    0.365

    rata-rata + SD

    0.36

    rata-rata + 2SD
    0.355

    rata-rata + 3SD
    Linear (rata-rata + SD)

    0.35

    Linear (rata-rata + 2SD)

    0.345
    0

    2
    Urutan Percobaan

    Linear (rata-rata + 3SD)

    Absorbansi Sampel Pengenceran II


    (300 L KMnO4/1000 L Aquadest)
    0.545
    0.54

    Y-Values

    Absorbansi

    0.535
    0.53

    rata-rata + SD

    0.525

    rata-rata + 2SD

    0.52

    rata-rata + 3SD

    0.515

    Linear (rata-rata + SD)

    0.51

    Linear (rata-rata + 2SD)

    0.505
    0

    Linear (rata-rata + 3SD)

    Urutan Percobaan

    Absorbansi Sampel Pengenceran III


    (400 L KMnO4/1000 L Aquadest)
    0.72

    Absorbansi

    0.71

    Y-Values

    0.7

    rata-rata + SD

    0.69

    rata-rata + 2SD

    0.68

    rata-rata + 3SD

    0.67

    Linear (rata-rata + SD)

    0.66

    Linear (rata-rata + 2SD)

    0.65
    0

    2
    Urutan Percobaan

    Linear (rata-rata + 3SD)

    Absorbansi

    Absorbansi Sampel Pengenceran IV


    (500 L KMnO4/1000 L Aquadest)
    0.88
    0.87
    0.86
    0.85
    0.84
    0.83
    0.82
    0.81
    0.8
    0.79
    0.78

    Y-Values
    rata-rata + SD
    rata-rata + 2SD
    rata-rata + 3SD
    Linear (rata-rata + SD)
    Linear (rata-rata + 2SD)
    0

    Linear (rata-rata + 3SD)

    Urutan Percobaan

    Pipet Piston
    Absorbansi Sampel Pengenceran I
    (200 L KMnO4/1000 L Aquadest)
    0.4
    0.35

    Y-Values

    0.3
    Absorbansi

    0.25

    rata-rata + SD

    0.2

    rata-rata + 2SD

    0.15

    rata-rata + 3SD

    0.1

    Linear (rata-rata + SD)

    0.05

    Linear (rata-rata + 2SD)

    0
    0

    2
    Urutan Percobaan

    Linear (rata-rata + 3SD)

    Absorbansi Sampel Pengenceran II


    (300 L KMnO4/1000 L Aquadest)
    0.52
    0.51

    Y-Values

    Absorbansi

    0.5
    0.49

    rata-rata + SD

    0.48

    rata-rata + 2SD

    0.47

    rata-rata + 3SD

    0.46

    Linear (rata-rata + SD)

    0.45

    Linear (rata-rata + 2SD)

    0.44
    0

    Linear (rata-rata + 3SD)

    Urutan Percobaan

    Absorbansi Sampel Pengenceran III


    (400 L KMnO4/1000 L Aquadest)
    0.86
    0.84

    Y-Values

    Absorbansi

    0.82
    0.8

    rata-rata + SD

    0.78

    rata-rata + 2SD

    0.76

    rata-rata + 3SD

    0.74

    Linear (rata-rata + SD)

    0.72

    Linear (rata-rata + 2SD)

    0.7
    0

    2
    Urutan Percobaan

    Linear (rata-rata + 3SD)

    Absorbansi Sampel Pengenceran IV


    (500 L KMnO4/1000 L Aquadest)

    Absorbansi

    0.86
    0.85

    Y-Values

    0.84

    rata-rata + SD

    0.83

    rata-rata + 2SD

    0.82

    rata-rata + 3SD

    0.81

    Linear (rata-rata + SD)


    Linear (rata-rata + 2SD)

    0.8
    0

    2
    Urutan Percobaan

    Linear (rata-rata + 3SD)

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