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    Development and Testing of Virus Induced Gene Silencing (VIGS) Vector For Banana (Musa Acuminata)

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    Nurulzahidah Arshad
    This document summarizes experiments testing virus-induced gene silencing (VIGS) vectors for banana plants using cucumber mosaic virus. It describes inoculating 10 banana plantlets that were 7 weeks old and 5 cm in height with the virus using carborundum tips as a mechanical inoculation method. After 7 and 30 days, it records the results of inoculation using carborundum or by scratching with tips. Finally, it provides status updates for different batches of inoculated and non-inoculated plants of varying ages and proposes next steps like finding a new inoculation site and practicing different inoculation methods.

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    Development and Testing of Virus Induced Gene Silencing (VIGS) Vector For Banana (Musa Acuminata)

    Uploaded by

    Nurulzahidah Arshad
    17 views8 pages
    This document summarizes experiments testing virus-induced gene silencing (VIGS) vectors for banana plants using cucumber mosaic virus. It describes inoculating 10 banana plantlets that were 7 weeks old and 5 cm in height with the virus using carborundum tips as a mechanical inoculation method. After 7 and 30 days, it records the results of inoculation using carborundum or by scratching with tips. Finally, it provides status updates for different batches of inoculated and non-inoculated plants of varying ages and proposes next steps like finding a new inoculation site and practicing different inoculation methods.

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    This document summarizes experiments testing virus-induced gene silencing (VIGS) vectors for banana plants using cucumber mosaic virus. It describes inoculating 10 banana plantlets that were 7 weeks old and 5 cm in height with the virus using carborundum tips as a mechanical inoculation method. After 7 and 30 days, it records the results of inoculation using carborundum or by scratching with tips. Finally, it provides status updates for different batches of inoculated and non-inoculated plants of varying ages and proposes next steps like finding a new inoculation site and practicing different inoculation methods.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
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    Download as pptx, pdf, or txt
    17 views8 pages

    Development and Testing of Virus Induced Gene Silencing (VIGS) Vector For Banana (Musa Acuminata)

    Uploaded by

    Nurulzahidah Arshad
    This document summarizes experiments testing virus-induced gene silencing (VIGS) vectors for banana plants using cucumber mosaic virus. It describes inoculating 10 banana plantlets that were 7 weeks old and 5 cm in height with the virus using carborundum tips as a mechanical inoculation method. After 7 and 30 days, it records the results of inoculation using carborundum or by scratching with tips. Finally, it provides status updates for different batches of inoculated and non-inoculated plants of varying ages and proposes next steps like finding a new inoculation site and practicing different inoculation methods.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
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    Development and Testing of Virus Induced Gene Silencing (VIGS) Vector for Banana (Musa acuminata)

    Nurulzahidah Binti Arshad SGF110021 Supervisor: Prof. Dr. Jennifer Ann Harikrishna Prof. Dr. Rofina Yasmin Othman

    Virus Host Test: Batch 3 Virus : CMV Method : Mechanical Inoculation Carborundum Tips Buffer : Phosphate buffer 1.0 M, ph 7.5 Number of sample : 10 plantlets Age / Height : 7 weeks / 5 cm

    2nd day of inoculation

    Method: carborundum

    Method: scratch using tip

    7th day of inoculation

    Method: carborundum

    Method: scratch using tip

    After 30th Day of Inoculation

    No of plants (Batch 1) 6 8

    Age/Height 6 months/ 20cm 6 months/ 20cm

    Current Status
    Inoculated (-ve result) (Carborundum/ Syringe)

    Not inoculated

    (Batch 2) 7 5
    (Batch 3) 9 3 10 10

    3 months/ 8-10cm
    3 months/ 8-10cm 7weeks/ 5cm 7weeks/ 5cm 2 weeks 1 week

    Inoculated ( Syringe) Not inoculated


    Inoculated (Carborundum) Not inoculated Not inoculated Not inoculated

    -Find new place for inoculation (C6) -Alingment of several Cucumber Mosaic Virus RNA 2 strain, Alingment of banana PDS gene with other monocoat plant. - Practise inoculation (injection/rubbing/Vacum inflitration) method

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