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Bacterial Physiology 2
Bacterial Physiology 2
Bacterial Physiology 2
Eri dian
Microbial Physiology
Nutritional factors for microbial growth Environmental factors for microbial growth Microbial metabolism
Metabolic Requirements
# Carbon source
- Autotrophs (lithotrophs): use CO2 as the C source Photosynthetic autotrophs: use light energy Chemolithotrophs: use inorganics - Heterotrophs (organotrophs): use organic carbon (eg. glucose) for growth.
# Nitrogen source
Ammonium (NH4+) is used as the sole N source by most microorganisms. Ammonium could be produced from N2 by nitrogen fixation, or from reduction of nitrate (NO3-)and nitrite (NO2).
Metabolic Requirements
# Sulfur source A component of several coenzymes and amino acids. Most microorganisms can use sulfate (SO42-) as the S source.
# Phosphorus source - A component of ATP, nucleic acids, coenzymes, phospholipids, teichoic acid, capsular polysaccharides; also is required for signal transduction. - Phosphate (PO43-) is usually used as the P source.
# Mineral source
- Required for enzyme function. - For most microorganisms, it is necessary to provide sources of K+, Mg2+, Ca2+, Fe2+, Na+ and Cl-. - Many other minerals (eg., Mn2+, Mo2+, Co2+, Cu2+ and Zn2+) can be provided in tap water or as contaminants of other medium ingredients. - Uptake of Fe is facilitated by production of siderophores (Iron-chelating compound, eg. Enterobactin). # Growth factors: organic compounds (e.g., amino acids, sugars, nucleotides) a cell must contain in order to grow but which it is unable to synthesize.
Organic coumpound
Organic nutrients are essential in different amounts, depending on the bacterial species. Carbohydrates are used as an energy source and as an initial substrate for biosynthesis of many substances. Amino acids are crucial for growth of some bacteria. Vitamins, purines and pyrimidines in trace amounts are needed for growth and as catalisator.
Essential Elements
Obligate aerobe
Microaerophile
Facultative anaerobe
Obligate anaerobe
Anaerobic jar
Anaerobic glove chamber
Redox Reactions
All chemical reactions consist of transferring electrons from a donor to an acceptor Chemicals that donate electrons become oxidized. Chemicals that accept electrons become reduced.
Redox Reactions
Energy is released during these electron transfers. In order to capture that energy, bacteria need to intercept the electrons during redox reactions Eh is important for clinical in infection wound whether aerob and anaerob population at the same site
Metabolism
The goal of metabolism is to conserve the energy released during redox reactions by making high energy compounds such as ATP. There are different strategies for conserving this energy
Metabolism
Metabolism to yield energy : 1. respiration 2. fermentation 3. photosynthetic Fermentation Transfer of electrons to organic substrate Respiration Transfer of electrons to inorganic acceptor
Phoptosynthesis : energy from light is altered to chemical energy in the form of ATP
PETA KONSEP
Energi
Proses sintesis sel dan enzim, memelihara steady state sel, penyerapan unsur hara, ekskresi senyawa, pergerakan sel
DEFINISI METABOLISME
Semua proses kimiawi yang dilakukan oleh organisme atau semua reaksi yang melibatkan transformasi energi kimia di dalam mahluk hidup
Definisi Metabolisme, Katabolisme dan Anabolisme Metabolisme sebagai proses produksi energi untuk kehidupan sel Senyawa pembawa energi, ATP dan ADP
Katabolisme: Penguraian senyawa yang menghasilkan energi (Reaksi eksergonik): RESPIRASI MENGURAIKAN KARBOHIDRAT MENJADI ASAM PIRUVAT DAN ENERGI
Chapter 5
Pergerakan (Motilitas)
ENERGI KIMIA
Komponen kimia berenergi tinggi:
Adenosin Diphosphate (ADP) dan Adenosine Triphosphate (ATP) yang dibentuk dari Adenosine Monophosphate
Bacteria Growth
Bacteria reproduce by a process called binary fission in which a parent cell divides to form a progeny of two cells This results in a logarithmic growth rate One bacterium will produce 16 bacteria after four generations. The doubling or mean generation time of bacteria may vary (e.g. 20 minutes for E. coli, 24 hours for M. tbc) The shorter the doubling time, the faster the multiplication rate Other factors that affect the doubling time include the amount of nutrients, the temperature and the pH of the environment
Bacterial growth
Principles of bacterial growth Bacteria multiply by binary fission.
A balance between slow loss of cells through death and the formation of new cells through growth and division.
The length of lag phase depends on the conditions in the original culture and the medium into which they are transferred.