Hema Star II

Technical information

Hema Star II S -A / rv.1.0

YSMAN067

Summary
SUMMARY 2

ELECTRICITY SUPPLY 1. 2. 3. 4. GENERAL REMARKS FIRST POWER SUPPLY SECOND POWER SUPPLY SCREEN

3 3 3 3 3 4 4 4 4 4 4 5 5 6 6 6 7 8 9 10

DESCRIPTION OF ELECTRONIC BOARDS 1. 2. 3. 4. 5. SYSTEM BOARD PREAMPLIFIER BOARD L10-CI-PRA20 PROCESSING BOARD L1O-PR-FIL3O M ECHANICAL / F LUIDIC MANAGEMENT BOARD L10-CI-DIL30 INTERFACE BOARD L1O-CI-INT-AA

SEQUENCING OF MAIN FLUIDIC CYCLES 1. 2. 3. 4. 5. 6. 7. DILUTION SEQUENCING COUNTING CYCLE RINSING AND DRAINAGE AT THE END OF COUNTING CYCLE (DILUTER) RINSING AND DRAINAGE AT THE END OF CYCLE (FLUIDIC ) SEQUENCING FIRST RINSING CYCLE SEQUENCING OF RINSING CYCLE EXCEPT THE FIRST SEQUENCING OF RINSING CYCLES AFTER 5 MINUTES

LIST OF ELECTRONIC SCHEMES

LIST OF FLUIDIC SCHEMES

10

Hema Star II S -A / rv.1.0

YSMAN067

Electricity supply
1. General remarks The device works on 10w voltage power from two sources in ventilated casing. The main supply element is Iocated at the back of the device preventing any risk of electrocution. Important: These partitioned power supplies do not have any accessible fuses. Contact your technical support. N.B: the presence of the different voltages displayed by leds on the interface board (appendix E3). 2. First power supply PC type power supply giving all boards the following voltages: +12V -12V +5V Power supply linked to the floppy disk driver and the system board, then to all other boards via the expansion slots and cable layers. It works on 50 or 60 Hz and there is a switch between the two EEC jacks for changing to 115 or 230V. 3. Second power supply Works on 30 VI 2 A, feeding the interface board. Accepts from 47 to 440 Hz and 2 voltage ranges, switched over by internal strap or automatic according to the version: - 95 to 132V -187 to 265V 4. Screen Works on fixed voltage 220 V, 50 to 60 Hz. On request, it may be supplied with different power supply voltages.

Hema Star II S -A / rv.1.0

YSMAN067

Description of electronic boards

Refer to block diagram : appendix E1. 1. System board Board of 80386 or 80486 type. The program, written in an advanced language, loaded in the core memory after power on. The dialog with the peripheral boards L10-CI-FIL-30 and L10-CI-DIL30 is in mode 1/0 by reciprocal interruption. 2. Preamplifier board L10-CI-PRA20 It polarizes the two counting probes and amplifies the signals. The hemoglobin determination system is on the preamplifier board. 3. Processing board L1O-PR-FIL3O Made up of three structures with independent Z80 microprocessors. Each structure has a specific task : counting of red corpuscles platelets white corpuscles / hemoglobin. The different signals are sorted out on peak width and height. 4. Mechanical / Fluidic management board L10-CI-DIL30 Two Z80 structures deal with the mechanical and fluidic management of the device. They control all the fluidic functions and all the motors following the program of the different cycles. 5. Interface board L1O-CI-INT-AA Power interface board which is linked to the management board by a 60 lead cable layer. Diluter Inputs / Outputs : needle mechanism liquid syringe from electrovalve EV1 to EV3 start button red and green leds to start the system up. Fluidics Inputs / Outputs : volumetric counting sensor and drainage optic sensor liquid micro-pump from electrovalve EV4 to EVI 2 air syringe

Hema Star II S -A / rv.1.0

YSMAN067

Sequencing of main fluidic cycles

1. Dilution sequencing

SYRINGE Sample 32 l BLOOD Amount 11 ml SAMPLE EXT. RINSING 3 ml ext. needle OF NEEDLE FIRST DILUTION 2 ml ext. needle 2 ml int. needle 1.5 ml ext. needle 1.5 ml int. needle to init DROP EXTRACTOR SAMPLE IN DILUTION 1

PHASE

NEEDLE Total ascent Descent 40 mm Ascent 15 mm Descent 15 mm Ascent 5 mm Ascent 5 mm Descent 15 mm Ascent 15 mm Descent 23 mm Ascent 29 mm

HOLDER

EV1 EV2 EV3

Init rinsing

Rinsing WBC

Flush elimination Sample 43.7 l Amount 10 ml

EXT. RINSING 1.5 ml ext. needle OF NEEDLE SECOND DILUTION

WBC rinsing Rinsing RBC

Descent 9 mm

6.5 ml ext. needle 1.5 ml int. needle RINSING LIQUID IN CHAMBER WC Amount 7 ml 7 ml ext. needle

REQUEST TRANSFER CHAMBER WBC + LYSIS

Ascent 5 mm RBC WBC

Amount 7 ml RINSING 6 ml ext. needle LIQUID IN CHAMBER RC 1 ml int. needle POSITION AWAIT OF COUNTING To switch

REQUEST TRANSFER CHAMBER RBC

WBC RBC

REQUEST COUNTING CYCLE

Descent 20 mm RBC rinsing

Hema Star II S -A / rv.1.0

YSMAN067

2. Counting cycle
FIRST COUNTING CHART

PHASE

SECOND COUNTING CHART THIRD COUNTING CHART

FOURTH COUNTING CHART

ACTION Pressure lowering with air syringe Injection of a bubble into the capillary Bubble passes in front of the start sensor Bubble passes in front of the end sensor Control of counting time Transfer of results WC, RC, PLT to PC Counting of duration determined by first chart Transfer of results WBC, RBC, PLT to PC Pressure lowering with air syringe Injection of a bubble into the capillary Bubble passes in front of the start sensor Bubble passes in front of the end sensor Control of counting time Transfer of results WC, RC, PLT to PC Counting of duration determined by third chart Hemoglobin measure Transfer of results WBC, RBC, PLT to PC

3. Rinsing and drainage at the end of counting cycle (diluter) PHASE

AFTER THE END OF COUNTING SYRINGE Backlash elimination NEEDLE HOLDER To slide Slide return Complete return EV1 EV2 EV3

Total ascent Descent 75 mm

READY FOR ANOTHER CYCLE 4. Rinsing and drainage at the end of cycle (fluidic) CHAMBER DIL. WBC
Transfer CHAMBER READY Transfer CHAMBER READY Drainage control CHAMBER DIL. RBC CHAMBER WBC Drainage CHAMBER RBC

Drainage

Drainage

Drainage Drainage control

Hema Star II S -A / rv.1.0

YSMAN067

5. Sequencing first rinsing cycle PHASE

1st DISTRIBUTION TO RINSING CHAMBER SYRINGE NEEDLE Ascent 50 mm HOLDER EV1 EV2 EV3
REQUEST CAPILLARY RINSING REQUEST DRAINAGE OF CHAMBERS WBC AND RBC

Amount 9 ml 4 ml ext. needle 4 ml int. needle

Descent 50 mm

Init rinsing

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER

2nd DISTRIBUTION TO Amount 9 ml RINSING CHAMBER 4 ml ext. needle 4 ml int. needle

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER

Amount 8 ml 1st DISTRIBUTION TO CHAMBER WBC

REQUEST DRAINAGE OF RINSING CHAMBER REQUEST DRAINAGE OF COUNTING CHAMBER WBC

Ascent 30 mm Descent 30 mm

Rinsing WBC

5 ml ext. needle 2 ml int. needle

Amount 8 ml 2nd DISTRIBUTION TO CHAMBER WBC 5 ml ext. needle 2 ml int. needle

REQUEST TRANSFER CHAMBER WBC REQUEST DRAINAGE OF COUNTING CHAMBER WBC

Ascent 30 mm Descent 30 mm

WBC RBC

Amount 9 ml 1st DISTRIBUTION TO CHAMBER RBC 6 ml ext. needle 2 ml int. needle

Amount 9 ml 2nd DISTRIBUTION TO CHAMBER RBC 6 ml ext. needle 2 ml int. needle

REQUEST TRANSFER CHAMBER RBC REQUEST DRAINAGE OF COUNTING CHAMBER RBC

Amount 9 ml 3rd DISTRIBUTION TO CHAMBER WBC

REQUEST TRANSFER CHAMBER RBC REQUEST DRAINAGE OF COUNTING CHAMBER RBC

6 ml ext. needle 2 ml int. needle

REQUEST TRANSFER CHAMBER WBC + LYSIS

REQUEST TRANSFER CHAMBER RBC REQUEST COUNTING CYCLE

Hema Star II S -A / rv.1.0

YSMAN067

6. Sequencing of rinsing cycle except the first PHASE

1st DISTRIBUTION TO RINSING CHAMBER SYRINGE Amount 5 ml NEEDLE HOLDER EV1 EV2 EV3
REQUEST CAPILLARY RINSING REQUEST DRAINAGE OF CHAMBERS WBC AND RBC

Total ascent Descent 50 mm

Init rinsing

2 ml ext. needle 2 ml ext. needle DISTRIBUTION TO CHAMBER WBC Amount 9 ml 6 ml ext. needle 2 ml int. needle DISTRIBUTION TO CHAMBER RBC Amount 9 ml 6 ml ext. needle 2 ml int. needle

REQUEST DRAINAGE OF NEEDLE RINSING CHAMBER

Ascent 30 mm

Descent 30 mm

Rinsing WBC

Ascent 30 mm
REQUEST TRANSFER CHAMBER WBC

WBC RBC

Descent 30 mm

REQUEST TRANSFER CHAMBER RBC REQUEST COUNTING CYCLE

Hema Star II S -A / rv.1.0

YSMAN067

7. Sequencing of rinsing cycles after 5 minutes PHASE

1st DISTRIBUTION TO CHAMBER WBC SYRINGE NEEDLE Total ascent HOLDER EV1 EV2 EV3
REQUEST CAPILLARY RINSING REQUEST DRAINAGE OF CHAMBERS WBC AND RBC

Amount 8 ml 5 ml ext. needle 2 ml ext. needle

Descent 50 mm

Init WBC

Amount 8 ml 2 DISTRIBUTION TO CHAMBER WBC

nd

REQUEST TRANSFER CHAMBER WBC

5 ml ext. needle 2 ml int. needle DISTRIBUTION TO CHAMBER RBC Amount 8 ml

REQUEST DRAINAGE OF CHAMBER WBC

Ascent 30 mm Descent 30 mm WBC RBC

5 ml ext. needle 2 ml int. needle

REQUEST TRANSFER CHAMBER WBC

REQUEST TRANSFER CHAMBER RBC REQUEST CAPILLARY RINSING REQUEST CAPILLARY RINSING MECHANICAL INITIALIZATIONS

Hema Star II S -A / rv.1.0

YSMAN067

List of electronic schemes

Appendix D1 : Appendix D2 : Appendix D21 : Appendix D3 : Appendix D31 : Appendix D4 : Appendix D41 : Preamplifier board L10-CI-PRA20 A/D conversion board L10-ANA20 Component location on conversion board Processing board L10-CI-FIL30 Component location on processing board Mechanical / Fluidic management board L10-CI-DIL30 Component location on mechanical / fluidic management board Appendix D5 : Interface board L10-CI-INT-AA Appendix D51 : Component location on interface board

Appendix E1 : Appendix E22 : Appendix E3 : Appendix E44 :

Machine block diagram Trimmer location on preamplifier board Trimmer location on interface board Trimmer location on A/D conversion board

List of fluidic schemes

Appendix F1 : Appendix F2 : Appendix F21 : Appendix F22 : Appendix F23 : Appendix F24 : Appendix F25 : Appendix F26 : Appendix F3 : Appendix F4 : Fluidic cabling Fluidic skeleton diagram WBC dilution transfer RBC dilution transfer Volumetric sensor rinsing (detergent circuit) Counting Drainage of counting chambers Diluter sequences Dismounting of ruby apertures Air and liquid syringe greasing

10

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D1
PREAMPLIFIER BOARD L10-CI-PRA20

11

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D2
A/D CONVERSION BOARD L10-ANA20

12

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D21
COMPONENT LOCATION ON CONVERSION BOARD

13

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D3
PROCESSING BOARD L10-CI-FIL30

14

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D31
COMPONENT LOCATION ON PROCESSING BOARD

15

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D4
MECHANICAL / FLUIDIC MANAGEMENT BOARD L10-CI-DIL30

16

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D41
COMPONENT LOCATION ON MECHANICAL / FLUIDIC MANAGEMENT BOARD

17

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D5
INTERFACE BOARD L10-CI-INT-AA

18

Hema Star II S -A / rv.1.0

YSMAN067

Appendix D51
COMPONENT LOCATION ON INTERFACE BOARD

19

Hema Star II S -A / rv.1.0

YSMAN067

Appendix E1
MACHINE BLOCK DIAGRAM

20

Hema Star II S -A / rv.1.0

YSMAN067

Appendix E22
TRIMMER LOCATION ON PREAMPLIFIER BOARD

21

Hema Star II S -A / rv.1.0

YSMAN067

Appendix E3
TRIMMER LOCATION ON INTERFACE BOARD

22

Hema Star II S -A / rv.1.0

YSMAN067

Appendix E44
TRIMMER LOCATION ON A/D CONVERSION BOARD

23

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F1
FLUIDIC CABLING

24

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F2
FLUIDIC SKELETON DIAGRAM

25

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F21
WBC DILUTION TRANSFER

Tubes concerns

EV contrles

26

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F22
RBC DILUTION TRANSFER

Tubes concerns

EV contrles

27

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F23
VOLUMETRIC SENSOR RINSING (DETERGENT CIRCUIT)

Tubes concerns

EV contrles

28

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F24
COUNTING

Tubes concerns

EV contrles

29

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F25
DRAINAGE OF COUNTING CHAMBERS

Tubes concerns

EV contrles

30

Hema Star II S -A / rv.1.0

YSMAN067

Appendix F26
DILUTER SEQUENCES

Blood or 2nd dilution extraction

Diluent loading

Needle external distribution

Needle internal distribution

31

Appendix F3
DISMOUNTING OF RUBY APERTURES

Rubis S -A / rv.2

Appendix F4
AIR AND LIQUID SYRINGE GREASING

33