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    Code: 9A23504 B.Tech III Year I Semester (R09) Supplementary Examinations, May 2013

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    sivabharathamurthy
    This document provides a summary of topics and questions for a genetic engineering exam, including gene regulation in prokaryotes and eukaryotes, types of plasmids, mechanisms of transposition and excision, enzymes used to manipulate DNA, cloning and screening using plasmid vectors, techniques like DNA hybridization and construction of genomic libraries, primer designing for PCR and its applications, RFLP and RAPD analysis, and potential hazards of genetic engineering. Students are instructed to answer 5 of the 8 questions provided.

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    Code: 9A23504 B.Tech III Year I Semester (R09) Supplementary Examinations, May 2013

    Uploaded by

    sivabharathamurthy
    7 views1 page
    This document provides a summary of topics and questions for a genetic engineering exam, including gene regulation in prokaryotes and eukaryotes, types of plasmids, mechanisms of transposition and excision, enzymes used to manipulate DNA, cloning and screening using plasmid vectors, techniques like DNA hybridization and construction of genomic libraries, primer designing for PCR and its applications, RFLP and RAPD analysis, and potential hazards of genetic engineering. Students are instructed to answer 5 of the 8 questions provided.

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    9A23504 Genetic Engineering

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    This document provides a summary of topics and questions for a genetic engineering exam, including gene regulation in prokaryotes and eukaryotes, types of plasmids, mechanisms of transposition and excision, enzymes used to manipulate DNA, cloning and screening using plasmid vectors, techniques like DNA hybridization and construction of genomic libraries, primer designing for PCR and its applications, RFLP and RAPD analysis, and potential hazards of genetic engineering. Students are instructed to answer 5 of the 8 questions provided.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    7 views1 page

    Code: 9A23504 B.Tech III Year I Semester (R09) Supplementary Examinations, May 2013

    Uploaded by

    sivabharathamurthy
    This document provides a summary of topics and questions for a genetic engineering exam, including gene regulation in prokaryotes and eukaryotes, types of plasmids, mechanisms of transposition and excision, enzymes used to manipulate DNA, cloning and screening using plasmid vectors, techniques like DNA hybridization and construction of genomic libraries, primer designing for PCR and its applications, RFLP and RAPD analysis, and potential hazards of genetic engineering. Students are instructed to answer 5 of the 8 questions provided.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
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    of 1

    Code: 9A23504 B.

    Tech III Year I Semester (R09) Supplementary Examinations, May 2013 GENETIC ENGINEERING
    (Biotechnology)

    R9
    Max Marks: 70 Answer any FIVE questions All questions carry equal marks
    *****

    Time: 3 hours

    1 2

    Discuss gene regulation and expression in Prokaryotes. Write in detail about the various stages at which regulation of genes takes place in eukaryotes. Write short on: Types of plasmids. Mechanism of transposition and excision. Explain the following: Enzymes used in manipulation of purified DNA. Cloning and screening in plasmid vector pBR . Explain the following techniques used in genetic engineering: DNA hybridization. Construction of genomic libraries. Describe in short about: Primer designing for PCR. Application of PCR technology. What do u means by RFLP and RAPD? Describe methodology and their applications. Humans are worried about the harmful effects caused by genetic engineering. State three potential hazards imposed by it.

    3 (a) (b) 4 (a) (b) 5 (a) (b) 6 (a) (b) 7 8

    *****

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