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Isolation and Identification Technique
Isolation and Identification Technique
Sukemi NIM. 1204016011 Management of Natural Resources and Renewable Energy Faculty of Forestry Mulawarman University 2013
GOALS
To investigate the antioxidant potential of polyphenolic rich ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn employing DPPH radical-scavenging, reducing power, Fe2+ chelating ability and lipid peroxidation assays
METHODS
EXTRACTION AND FRACTINATION BY INCREASING ORDER OF SOLVENT POLARITY
METHODS
EXTRACTION AND FRACTINATION BY DECREASING ORDER OF SOLVENT POLARITY
METHODS
Determination of total phenolics Antioxidant testing assays DPPH scavenging assay Reducing power assay
RESULTS
TOTAL FENOLIC CONTENT
Water fraction has a higher phenolic content (720 mg) than ethyl acetate fraction (600 mg) and crude ethyl acetate extract (390 mg) expressed as gallic acid equivalents (GAE)/g of extract/fraction
ANTIOXIDANT ACTIVITY DPPH SCAVENGING
CHELATING POWER
The difference in the antioxidant activity of the water, ethyl acetate fractions and crude extract may be ascribed to the difference in the total phenolic content as well as the phenolic compositions.