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Bay scallop genetic diversity

and population structure in


Massachusetts

Steven Roberts
School of Aquatic and Fishery Sciences
University of Washington
Outline

•Beginning
• Marker Development
• Populations / Enhancement
• Conclusions
Library Characteristics NCBI Present Status

Individual clones sequenced


D-hinge 671
• ESTs = 7057
Met 61
Set 932
Adductor 367
• CoreNucleotides = 85
Gonad 58
8% sequences <200 bp
Average length - 719 bp
970 singletons
217 contigs
• Contigs 845
• Singletons 2746
• Unique Seqs 3591
Enhancement
Population Differences

Self-assignment
Only Adults
Random = 11%
Chatham = 60%
Assessing Enhancement

private alleles
showing up post
enhancement
Summary
• Identify markers that effectively identify
all potential parent populations
• functional markers (SSR-ESTs, SNPs)
• Isolation coupled with life history can
dramatically affect genetic diversity
• Temporal (yearly) variation is interesting
and critical for understanding diversity

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