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    Isolation of Gram Positive Organisms-1

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    Justin Cebrian
    The document provides instructions for isolating and identifying gram positive organisms using various culture media and diagnostic tests. It describes the different types of media that can be used like blood agar, chocolate agar, and mannitol salt agar and explains how to perform and interpret catalase, coagulase, and mannitol fermentation tests to differentiate between organisms like Staphylococcus and Streptococcus. The goal is to select the appropriate media and tests to isolate and identify common gram positive bacteria.

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    Isolation of Gram Positive Organisms-1

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    Justin Cebrian
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    The document provides instructions for isolating and identifying gram positive organisms using various culture media and diagnostic tests. It describes the different types of media that can be used like blood agar, chocolate agar, and mannitol salt agar and explains how to perform and interpret catalase, coagulase, and mannitol fermentation tests to differentiate between organisms like Staphylococcus and Streptococcus. The goal is to select the appropriate media and tests to isolate and identify common gram positive bacteria.

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    The document provides instructions for isolating and identifying gram positive organisms using various culture media and diagnostic tests. It describes the different types of media that can be used like blood agar, chocolate agar, and mannitol salt agar and explains how to perform and interpret catalase, coagulase, and mannitol fermentation tests to differentiate between organisms like Staphylococcus and Streptococcus. The goal is to select the appropriate media and tests to isolate and identify common gram positive bacteria.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    5 views17 pages

    Isolation of Gram Positive Organisms-1

    Uploaded by

    Justin Cebrian
    The document provides instructions for isolating and identifying gram positive organisms using various culture media and diagnostic tests. It describes the different types of media that can be used like blood agar, chocolate agar, and mannitol salt agar and explains how to perform and interpret catalase, coagulase, and mannitol fermentation tests to differentiate between organisms like Staphylococcus and Streptococcus. The goal is to select the appropriate media and tests to isolate and identify common gram positive bacteria.

    Copyright:

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    Download as PPT, PDF, TXT or read online from Scribd
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    of 17

    Isolation of Gram Positive Organisms

    Exercise

    Culture Media for Isolation of Gram Positive Organism

    BAP

    CAP

    MSA

    Blood Agar
    5% defibrinated sheep, horse, or rabbit blood + TSA Soy Peptone Blood

    AA, C, N, and S

    Detect hemolysis

    Types of Hemolysis

    Alpha Incomplete or partial Greening

    Beta Complete Colorless, clear zone

    Gamma No hemolysis

    Beta Hemolysis

    Gamma Hemolysis

    Chocolate Agar

    Allow growth of fastidious organisms


    Hemolysis or no hemolysis

    CAP

    Catalase Test
    Catalase converts H2O2 into H2O and O2
    Differentiate Staphylococcus and Streptococcus

    Interpretation

    Positive

    Negative

    Coagulase Test
    Coagulase convers soluble fibrinogen to insoluble fibrin S. aureus

    Purpose

    Forms of Coagulase

    Bound
    Slide test

    Free
    Tube test

    Interpretation

    Negative

    Positive

    Mannitol Fermentation Test

    MSA

    Selective

    Differential

    Principle
    High levels of NaCl (7.5%)

    Beef extract, D-mannitol, phenol red

    Differentiate S. aureus and S. epidermidids

    Mannitol Fermenter

    Nonmannitol Fermenter

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