1.

van den Bergh test, reaction a test which differentiates between conjugated and unconjugated bilirubin in serum and assists in the differentiation between biliary or regurgitation hyperbilirubinemia, retention or hemolytic hyperbilirubinemia, or combined hyperbilirubinemia or jaundice. There are many variants of the basic test and the interpretation of them differs markedly between the species. http://medical-dictionary.thefreedictionary.com/van+den+Bergh+test

2. Test principle a. Jendrassik-Grf method The azobilirubin produced by the reaction between bilirubins and the diazonium salt of sulfanilic acid shows maximum absorption at 555 nm in an acid medium. The intensity of the colour produced is proportional to the quantity of bilirubin which has reacted. In the absence of an accelerator, only conjugated bilirubins react. http://www.mti-diagnostics.com/produktbeschreibung/bulkware/bilirubin-direct-lsjendrassik-grof-method/index.html B. Malloy and Evelyn Reaction In an aqueous solution, Ehrlich's diazo reagent reacts with the direct bilirubin in the serum to form a pink to reddish-purple colored compound (azobilirubin). It is read at one minute. In a 50% methyl alcohol solution, Ehrlich's diazo reagent reacts with the total bilirubin in the serum to form a pink to reddish-purple colored compound. (Read at 30 minutes.) http://www.2ndchance.info/dxme-BilirubinUrine-defDirect.pdf