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50000

Q
40000

Q
30000

C
20000 10000 0 0

C P P Q P

10 20 Uninoculated Medium

30

40 50 Treated Medium

60 70 before treatment

80

Figure 1 X-ray diffraction patterns of mineral sulfidic ore before and after bioleaching experiment with mixotrophic bacteria C: Clinochlore ((Mg,Fe2+)5Al(Si3Al)O10(OH)); Q: Quartz (SiO2); and P: Pyrite (FeS2). Units of Y axis are arbitraries. Mineral sulfide ore for bioleaching experiment was from Cipatujah, West Java Province. Mineral that could be detected by Xpowder software consisted of Clinochlore ((Mg,Fe2+)5Al(Si3Al)O10(OH)), Quartz (SiO2), and Pyrite (FeS2). Pyrite has been known worldwide as a dominant gangue metal in mineral sulfide ores. This mineral was also associated with acid mine drainage formation. X-ray diffraction patterns of mineral sulfidic ore before and after the bioleaching experiment are shown in Fig. 1 The main mineralogical species in the untreated ores are clinochlore, pyrite and quartz. After the treatment, intensities of the peaks of quartz and clinochlore intensively increased, indicating that the gangue of this ore was partially solubilized during the leaching. The presence of new peaks and those species after the leaching suggests a partial oxidation of iron in solution favoured by the consequent precipitation of ferric (or ferric/ferrous) iron oxides.

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