.

) (




PH PH .




.

))

(( .

 ..

 Purification Of
Enzymes :
-1 Molecular Precipitation
-2 Adsorption .
-3 Polymerization
-4 Centrifugation.
-5 . Electrophoresis
-

 : IU
1
30
.

 :
.

.
:
. .
. ) (

 )



(

Cofactor .

( CO- enzymes)

.


.B


.
Coenzyme
Prosthetic
group
.

Apoenzyme
Holoenzyme



.

,
ACTIVE SITE
) ( Substrate
.

,
,

 Specificity of enzymes
:


)Absolute
(specificity )
) Urease
Low Specificity

Lipase

Stereo-chemical.
Specificity

- .

- .

- ) pro

 :


.
)) (( " " ase

) (
.
)) (( " " ase

1961
"ase
"
a Systematic Code number for each

enzyme

] [ E . C 4
.

 ) )3.1.1.3 } {3
) (3 } {1
) (1 ) (1.3 }{1
) (
) (3.1.1 } {3




.3.1.1.3

 6
:

1961
.

_ 1
Oxidoreductases


.
:
.
.
.
.

 Phenol-oxidase


) ( o-Diphenoloxidase:EC1.10.3.1 )p- Diphenoloxidase :
. ( EC 1.10.3.2

Quinone
Melanin

( ):( Catalase
(Catalase (EC 1. 11.1.6

) (H2O2
:
2 H2O + O2

Catalase

H2O2

( : Peroxidase
(Peroxidase (EC 1.11.1.7


) (H2O2 :

Peroxidase
+
2H2O

H2O2 A

AH2

_ 2
Transferases

.
.

) EC 2.4.1.1) Phosphorylase

) Dextrins

 - 3 )( Hydrolases

.
)( ) EC 3.2.1.26) Invertase
)Glucose 1-2
(Fructose ) (
) ( :

 _ 3
) ( Hydrolases

 - 4 Lyases

) (


)(Aldolase 16

 - 5 Isomerases


.

 - 6 Ligases


) (ATP

.
) 20 - (

 )( PH
PH


)inhibitor

( Competitive

.






.

.2

)Non Competitive

(Inhibitor








.
.