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MIC by Broth Dil
MIC by Broth Dil
MIC by Broth Dil
WHY?
Clinically, MIC is not only used to determine the amount of antibiotic that the patient will receive but also the type of antibiotic used. This will lower the opportunity for microbial resistance to specific antimicrobial agents.
Detailed procedure
Materials:
7 Wassermann tubes (Mark 1-6 Last: control) Test Tube with 10 ml of double strength nutrient broth Tube with 2 ml of AB solution Tube with Microorganism Culture One Sterile 5 ml pipette 1 Sterile 1 ml pipettes One sterile Pasteur pipette
Detailed procedure
If AB conc is 400ug/ml
1
1X 200
2
100
3
50
4
1/8 25
5
1/16 12.5
6
1/32 6.25
1- Aseptically distribute 1ml broth into each wasserman tube with 5 ml pipette
21Add 1ml AB in the first wasserman tube, mix and transfer 1 ml from tube 1 to 2, repeat till tube 6 using 1 ml pipette 3- Inoculate all tubes with one drop of the microorganism using
Precautions
Mark carefully all tubes. Adjust the volume in all tubes Dont open the broth tube more than 2 times !!DO NOT ADD AB TO THE CONTROL TUBE!!
Mixing should be at the bottom of the tube? To avoid air bubbles ?? Contamination !!!!!!!!
Expected results