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  • Coupled Rna Splicing

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    Pandit Ji
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    The document describes the coupled process of RNA splicing and nuclear export. It shows a pre-mRNA undergoing splicing by the spliceosome to remove an intron and join two exons. Splicing factors then package the mRNA and target it to the nuclear pore complex for export to the cytoplasm, with Aly bridging the exon junction complex to the export receptor. Factors involved in nonsense-mediated decay remain bound following export.

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    The document describes the coupled process of RNA splicing and nuclear export. It shows a pre-mRNA undergoing splicing by the spliceosome to remove an intron and join two exons. Splicing factors then package the mRNA and target it to the nuclear pore complex for export to the cytoplasm, with Aly bridging the exon junction complex to the export receptor. Factors involved in nonsense-mediated decay remain bound following export.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
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    Download as ppt, pdf, or txt
    16 views2 pages

    Coupled Rna Splicing

    Uploaded by

    Pandit Ji
    The document describes the coupled process of RNA splicing and nuclear export. It shows a pre-mRNA undergoing splicing by the spliceosome to remove an intron and join two exons. Splicing factors then package the mRNA and target it to the nuclear pore complex for export to the cytoplasm, with Aly bridging the exon junction complex to the export receptor. Factors involved in nonsense-mediated decay remain bound following export.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
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    Coupled RNA Splicing and Nuclear Export

    sigma-aldrich.com/cellsignaling

    Coupled RNA Splicing and Nuclear Export

    In the schematic, a simplified pre-mRNA with a 5 cap, 2 exons, a single intron, and a poly(A) tail is shown with the spliceosome. The splicing factor UAP56 (a helicase and DEAD box containing protein family member) associates with Aly and the spliceosome. Heterogeneous nuclear ribonucleoproteins (hnRNPs) package the pre-mRNA and SerArg Rich (SR) proteins associate with exons. Next, the TAP/p15 heterodimer (Mex67/TAP-Mtr2/p15 RNA export receptor) targets the mRNP to the nuclear pore complex (NPC), with Aly acting as a bridge between the exon junction complex (EJC) and the TAP/p15 complex. Finally, mRNA export factors dissociate from the mRNP following export to the cytoplasm. Factors involved in nonsense-mediated mRNA decay (NMD, a conserved mechanism for the detection and elective degradation of mRNAs with premature stop codons) (Upf3, Y14, and RNPS1) remain bound to the mRNP. Adapted from Reed et al, Cell 108:523, 2002.

    sigma-aldrich.com/cellsignaling

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