Botany 251Y Part II Powerpoint Animations for the Photosynthesis lectures

Directions: Play the Powerpoint show, and click enter to move to the next step in the animation, or the next slide.

LIGHT ABSORBTION AND TRANSFER TO THE REACTION CENTERS

NADP reductase

4 photons
4 photons

2H+ + 2NADP
NADPH PQ

2 H+

Fd

CYT B6f

PSII

O2 + 4H+
2H2O
PC 4 e-

PSI

The Path of Electron and Proton Flow in Photosynthetic Electron Transport

Under conditions where NADP+ regeneration is slow, or ATP demand is high, the leaf can cycle electrons between plastoquinone and PSI, and in doin so pump protons across the membrane. This is termed cyclic photophosphrylation.

NADP reductase 2 H+ PQ CYT B6f

Fd

PSII

PC 4 H+

PSI

CYCLIC PHOTOPHOSPHORYLATION

H+

H+

PHOTOPHOSPHORYLATION
Chloroplast stroma region of high pH
H+
H+

ATP Synthase (F-type ATPase)

H+ H+ H+

H+
H+ H+

H+

H+

ADP + Pi

ATP

H+

PHOTOSYSTEM I

H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+

H+ H+H+ H+ H+ H+H+ H+ + H+ 3 H+H+ H+ H H+ H+H+ H+

H+ H+ H+ H+ H+ H+ H+ H+

H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+ H+

H+ H+ H+ H+ H+ H+ H+ H+

H+ H+ H+ H+ H+ H+ H+ H+ + H+ H+ H+ H H+ H+ H+ H+

H+ H+ H+ H+ H+ H+ H+ H+

Thylakoid Lumen compartment of low pH

Phase 1: Carboxylation
RuBP (5 carbon) + CO2
RUBISCO

2 PGA (3 carbon)

Note: The oxygen in CO2 is incorporated into one of the PGA molecules. It is not released as O2.

Properties of Rubisco
(Ribulose-1,5-bisphosphate carboxylase/oxygenase)
In primitive photosynthetic bacteria, Rubisco exists as a dimer of two subunits

In the evolution of the bluegreen algae, the primitive, two-subunit form of Rubisco was modified by the combination of 4 dimers to give a complex of 8 subunits in four pairs of dimers.

RuBP Oxygenation
RuBP (5 carbon) + O2 PGA + PG

RUBISCO