3

(Determination of Chemical Properties of

Lipid)

1.

2.
3.

1.

2.

3.

4. (hydrolysis)

5/2

2
1. (simple lipid)

Fats (oil)

2. (complex lipid)

(phospholipid)

(glycolipid)

1. (saturated fatty acid) steric

acid, palmitic acid

2. (unsaturated fatty acid)

oleic acid, linoleic acid

(Rancidity)

5/3

Thin layer chromatography (TLC)

Thin layer chromatography (TLC)

(mobile phase)
(stationary phase)

TLC

5.1

chromatography

Thin layer

(Thin layer chromatography)

Rf

5.1.1)

1.
2. hexane

3. vortex

4. water bath 60 C
5. hot plate

5/4

5.1.2) Thin layer chromatography

1. TLC ( 40 % silica gel)

2. TLC tank

3. chloroform : acetone

4.

(96 : 4) (v/v)

Triolein

Oleic acid

Chloresterol

5.1.1)

1. 1 hexane 5 .

hexane

2. water bath 60
3

3. vortex
4.

hexane

2-3 3

6. hexane

7. hexane hot plate

8. hexane 3
5.1.2

5.1.2) Thin layer chromatography

1. chromatographic tank
chloroform acetone 1 .
( hood )

5/5

2. TLC

3. 5.1.1

capillary tube

5 1

4. triolein, oleic acid cholesterol

capillary tube 2 2, 3 4

5. TLC

6. chromatographic tank
TLC 1-2 .

7. TLC chromatographic tank

8. Rf

5.2

(nonpolar)

micelles (hydrophobic)
nonpolar (hydrophilic) polar
(water phase)

1.

2. , acetone, 1% albumin

1. 4

2. 1 .

5/6

1
2
3

3.

acetone

1% albumin

4.

5.
5.3

(unsaturation test)

( )

CH3-(CH2)7-CH=CH-(CH2)7-COOH +
Br2
CH-CH-(CH2)7-COOH
Br

1.
2. oleic acid

3. steric acid
4. chloroform

5. 10 %

Br
CH3-(CH2)7-

5/7

1. 4

2. chloroform 1 .
3. oleic acid steric acid 3

4.
5.

6.

5.4

1.

2.

chloroform

4.

acetic acid

3.
5.

methanol
10 % KI

1.
2.

chloroform 0.5 .

5/8

3.
4.

methanol 0.5 .

10 1 10

2
5.

acetic acid

6.

10 % KI

(CH3COOH)

2 .

1 5

7.

8.

1. chloroform : acetone
chloroform

2. 10 % KI

50 .

(96 :4)

96 . acetone 4 .

patassium iodide (KI)

5
50 .

*************************************************************************************
*********************************************

1. (2540)

5/9

2. (2542) 3

3. (2538)

5.1

Thin layer

chromatography

(.)

Triolein
Oleic acid

cholesterol

1
2
3
4

(.)

Rf

5/10

TLC

.
..

5/11

.
5.2

+
++ +++ +

Acetone
1% albumin

.
5.3

5/12

Oleic acid

Steric acid

..
5.4

+ ++ +++ I2

I2

5/13