Scribd Logo
Upload

Welcome to Scribd!

  • 26 views

    Dna Replication: Origins of Replication: Prokaryotes: Single, Eukaryotes: Multiple

    Uploaded by

    Chiranjit Debbarma
    DNA replication involves three main steps: initiating replication at origins of replication which are single in prokaryotes and multiple in eukaryotes, forming replication bubbles and forks; priming using short RNA primers made by primase to allow DNA polymerase to synthesize new DNA strands; and elongation as DNA polymerase adds nucleotides continuously on the leading strand but discontinuously in 100-200 nucleotide fragments on the lagging strand that are later joined by ligase.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content

    You might also like

    Dna Replication: Origins of Replication: Prokaryotes: Single, Eukaryotes: Multiple

    Uploaded by

    Chiranjit Debbarma
    26 views1 page
    DNA replication involves three main steps: initiating replication at origins of replication which are single in prokaryotes and multiple in eukaryotes, forming replication bubbles and forks; priming using short RNA primers made by primase to allow DNA polymerase to synthesize new DNA strands; and elongation as DNA polymerase adds nucleotides continuously on the leading strand but discontinuously in 100-200 nucleotide fragments on the lagging strand that are later joined by ligase.

    Original Description:

    genetics

    Original Title

    DNA Replication

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    DNA replication involves three main steps: initiating replication at origins of replication which are single in prokaryotes and multiple in eukaryotes, forming replication bubbles and forks; priming using short RNA primers made by primase to allow DNA polymerase to synthesize new DNA strands; and elongation as DNA polymerase adds nucleotides continuously on the leading strand but discontinuously in 100-200 nucleotide fragments on the lagging strand that are later joined by ligase.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    26 views1 page

    Dna Replication: Origins of Replication: Prokaryotes: Single, Eukaryotes: Multiple

    Uploaded by

    Chiranjit Debbarma
    DNA replication involves three main steps: initiating replication at origins of replication which are single in prokaryotes and multiple in eukaryotes, forming replication bubbles and forks; priming using short RNA primers made by primase to allow DNA polymerase to synthesize new DNA strands; and elongation as DNA polymerase adds nucleotides continuously on the leading strand but discontinuously in 100-200 nucleotide fragments on the lagging strand that are later joined by ligase.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 1

    Processes 1.

    Initiating replication Origins of replication: Prokaryotes: single, Eukaryotes: multiple Replication bubble - Replication proceeds in both directions Replication fork - Where new strands of DNA elongate Helicase - Untwists and separates DNA strands Binding proteins - Stabilize strands 2. Priming Reason: DNA polymerase cant synthesize nucleotides from scratch Primer - Short stretch of RNA Primase - Makes primer DNA polymerase - Replaces RNA primers with DNA Antiparallel strands 3. Elongation DNA polymerase - Can only add nucleotides to the free 3 end DNA elongation: 5-3 direction Leading strand - Continuous synthesis; require only one primer Lagging strand synthesis - Discontinuous synthesis Okazaki fragments - 100-200 nucleotides long; each requires a primer Ligase - Joins Okazaki fragments

    DNA REPLICATION

    You might also like