RMX ReaPan Micro Reagent

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ReaPan Micro

First of its kind in India,


ReaPan Micro is a rapid and
sensitive test for bacterial
screening of urine samples
using flow cytometry.

Now, no more time consuming


routine cultures;
rule out bacterial infection
within minutes.

Features
Results in less than 15 minutes, in com-
parison to routine cultures that
take between 24-48 hours.

A Negative Predictive Value (NPV)


greater than 99%.

Intensity normalization using


intensity control beads
for precision.

Assurance of a simplified
assay workflow.

Validated extensively.

The test uses a nucleic acid stain, Measurement of fluorescent event


Thiazole Orange, to stain the bacteria counts lesser than a prescribed
in pre-filtered urine. An optimized threshold, indicates the absence of
gating strategy together with use of bacteria in a sample.
intensity control and absolute counting
beads enables enumeration of stained Ph: +91-80-28378693/5
fluorescent events, on a flow cytometer. E-mail: info@reametrix.com
Web: www.reametrix.com
Optimized Gating Strategy
Less Than 10,000 Cfu/Ml
Culture Negative

14.70 Counts/uL
More Than 100,000 Cfu/Ml
46805.29 Counts/uL
Culture Positive

Bacterial population gated on Absolute counting bead Bacterial population counted


SSC-FL1 dot plot. population gated on on FL1 Histogram.
FL2-FL3 dot plot.

Sample Workflow

ReaMetrix
1 2
Pipette 1ml of filtered
Filter 2ml of urine urine sample from Accelerating Innovation

sample into eppendorf eppendorf into ReaPan


Micro FACSTube. 50-B, II Phase
tube using enclosed
syringe filter
Peenya Industrial Area
Peenya, Bangalore 560058, India

Ph: +91-80-28378693/5
Fax: +91-80-41172451

E-mail: info@reametrix.com
3 4 Web: www.reametrix.com

Vortex & incubate


sample at room Acquire
temperature
Optimized and Simple for 5
Gating Strategy data on flow
minutes. cytometer
with vortexed
patient sample
using preset
template. DIN EN ISO 9001:2000
Certificate No 01 100 058860

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