Growth on MacConkey Agar No Growth on Rose Agar Gram Negative Rod Escherichia coli Enterobacter aerogenes Klebsiella pneunomiae

Serratia marcescens Proteus vulgaris Salmonella typhirium Pseudomonas aeruginosa Alcaligenes faecalis MacConkey Agar Growth +/Acid + Escherichia coli Enterobacter aerogenes Klebsiella pneunomiae Serratia marcescens Indole Growth Proteus vulgaris Salmonella typhirium Pseudomonas aeruginosa Alcaligenes faecalis Indole and/or Urease

Enterobacter aerogenes Klebsiella pneunomiae Serratia marcescens

+
Escherichia coli

Salmonella typhirium Pseudomonas aeruginosa Alcaligenes faecalis

+
Proteus vulgaris

Urease + Klebsiella pneunomiae Enterobacter aerogenes *Serratia marcescens *Serratia marcescens Motility + Serratia marcescens Klebsiella pneunomiae Gelatin Hydrolysis + Serratia marcescens

Methyl Red + Salmonella typhirium Pseudomonas aeruginosa Alcaligenes faecalis NO3 Reduction + Pseudomonas aeruginosa Alcaligenes faecalis

Enterobacter aerogenes

*Differs among strain Confirming tests for E. coli: EMB plate Metallic Green color Confirming tests for P. vulgaris and S. typhirium: H2S production + Confirming test for P. vulgaris: Urease + Confirming tests for S. typhimurium: HE agar Green, non-lactose fermenting colonies with a black center Confirming tests for S. marcescens: DNAse + @ 25C, Red pigment on TSA @ 25C Confirming tests for K. pneumoniae: Encapsulated, appears as mucoid colonies that tend to string when lifted with a loop, Citrate +, pink mucoid colonies on MacConkey agar Confirming tests for E. aerogenes: VP +. Citrate +, Motility +, ferments everything! Confirming tests for P. aeruginosa: Oxidase +, Nitrates reduced to Nitrites, Citrate +, Growth @ 42C +, Motile Confirming tests for A. faecalis: Oxidase +, Growth in 6.5% NaCl +