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  • 5X TBE (1.1M Tris 900mM Borate 25mM EDTA PH 8.3) : Precipitation Will Form Over Time, But Buffer Is Gererally Still

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    Andreea Lulea
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    The document provides instructions for making 5X TBE buffer in volumes of 8L and 2L. It lists the reagents, amounts needed, and catalog numbers. The reagents (Tris, boric acid, and EDTA) are dissolved in water at 75% of the final volume. The pH is then adjusted to 8.3 with HCl and the volume brought up to the final amount. The 5X buffer can be stored at room temperature and remains usable despite potential precipitation over time. A 0.5X working solution is made by diluting 100mL of the 5X buffer in 900mL water.

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    Protocol 7

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    The document provides instructions for making 5X TBE buffer in volumes of 8L and 2L. It lists the reagents, amounts needed, and catalog numbers. The reagents (Tris, boric acid, and EDTA) are dissolved in water at 75% of the final volume. The pH is then adjusted to 8.3 with HCl and the volume brought up to the final amount. The 5X buffer can be stored at room temperature and remains usable despite potential precipitation over time. A 0.5X working solution is made by diluting 100mL of the 5X buffer in 900mL water.

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    29 views2 pages

    5X TBE (1.1M Tris 900mM Borate 25mM EDTA PH 8.3) : Precipitation Will Form Over Time, But Buffer Is Gererally Still

    Uploaded by

    Andreea Lulea
    The document provides instructions for making 5X TBE buffer in volumes of 8L and 2L. It lists the reagents, amounts needed, and catalog numbers. The reagents (Tris, boric acid, and EDTA) are dissolved in water at 75% of the final volume. The pH is then adjusted to 8.3 with HCl and the volume brought up to the final amount. The 5X buffer can be stored at room temperature and remains usable despite potential precipitation over time. A 0.5X working solution is made by diluting 100mL of the 5X buffer in 900mL water.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    of 2

    5X TBE(1.1M Tris; 900mM Borate; 25mM EDTA; pH 8.

    3)

    REAGENT disodium EDTA Trizma Base Boric Acid

    8L 37.5g 432g 220g

    2L 9.375g 108 g 55g

    Sigma Aldrich Cat# E-5134 T-1503 B-0252

    In an appropriately sized beaker, dissolve Tris, Boric Acid and EDTA in a volue of distilled deionized water that is equal to about 75% of the volume of 5X buffer to be made. Add a large magnetic stirbar and place slurry on stirplate on high to facilitate mixing and dissolving of chemicals. EDTA may be slow to dissove, but low heat can be applied to facilitate dissolution. Once dissolved, add water to just below the desired volume. pH must then be adjusted to 8.3 by adding concentrated HCl, using a pH meter to constantly monitor the pH. There is no need to sterilize the solution and it can be stored at room temperature. Precipitation will form over time, but buffer is gererally still usable

    0.5X TBE Electrophoresis Buffer for 1L 5X TBE 100 mL Distilled, deionized water 900 mL In an erlynmeyer flask, add 100mL of 5X TBE to 900mL of distilled deionized water. Mix well and use. NOTE: In this lab, 5X TBE buffer is premade. You will only be required to dilute the 5X buffer to 0.5X for use.

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