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    S-173 IC Application Note No.: Title: Nitrite and Nitrate in A Plant Extract

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    This document summarizes a method for determining nitrite and nitrate concentrations in a plant extract using anion chromatography with conductivity detection and chemical suppression. The plant extract was diluted and injected into a column with 1 mmol/L sodium hydrogen carbonate and 3.2 mmol/L sodium carbonate as the eluent. Suppression was achieved using a suppressor module containing 100 mmol/L sulfuric acid. This allowed for the separation and quantification of nitrite and nitrate, with results of 0.37 mg/L nitrite and 10.52 mg/L nitrate found in the sample.

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    S-173 IC Application Note No.: Title: Nitrite and Nitrate in A Plant Extract

    Uploaded by

    829255
    18 views1 page
    This document summarizes a method for determining nitrite and nitrate concentrations in a plant extract using anion chromatography with conductivity detection and chemical suppression. The plant extract was diluted and injected into a column with 1 mmol/L sodium hydrogen carbonate and 3.2 mmol/L sodium carbonate as the eluent. Suppression was achieved using a suppressor module containing 100 mmol/L sulfuric acid. This allowed for the separation and quantification of nitrite and nitrate, with results of 0.37 mg/L nitrite and 10.52 mg/L nitrate found in the sample.

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    696030_AN-s173

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    This document summarizes a method for determining nitrite and nitrate concentrations in a plant extract using anion chromatography with conductivity detection and chemical suppression. The plant extract was diluted and injected into a column with 1 mmol/L sodium hydrogen carbonate and 3.2 mmol/L sodium carbonate as the eluent. Suppression was achieved using a suppressor module containing 100 mmol/L sulfuric acid. This allowed for the separation and quantification of nitrite and nitrate, with results of 0.37 mg/L nitrite and 10.52 mg/L nitrate found in the sample.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    18 views1 page

    S-173 IC Application Note No.: Title: Nitrite and Nitrate in A Plant Extract

    Uploaded by

    829255
    This document summarizes a method for determining nitrite and nitrate concentrations in a plant extract using anion chromatography with conductivity detection and chemical suppression. The plant extract was diluted and injected into a column with 1 mmol/L sodium hydrogen carbonate and 3.2 mmol/L sodium carbonate as the eluent. Suppression was achieved using a suppressor module containing 100 mmol/L sulfuric acid. This allowed for the separation and quantification of nitrite and nitrate, with results of 0.37 mg/L nitrite and 10.52 mg/L nitrate found in the sample.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    IC Application Note No.

    S-173
    Title:
    Summary:

    Nitrite and nitrate in a plant extract


    Determination of nitrite and nitrate in a plant extract using anion chromatography with conductivity detection after chemical suppression. Plant extract

    Sample:

    Sample Preparation: Dilution 1: 80 with ultrapure water Column: Eluent: Suppressor: Flow: Injection Volume:
    mV 15

    6.1006.530 Metrosep A SUPP 5 250 1.0 mmol/L sodium hydrogen carbonate 3.2 mmol/L sodium carbonate Metrohm Suppressor Module (MSM, 100 mmol/L H2SO4) 0.7 mL/min 20 L

    10

    0.02 S/cm

    0 nitrate ch1 -10 nitrite 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

    -5

    -15

    -20

    -25 min

    Results:

    Nitrite mg/L 0.37

    Nitrate mg/L 10.52

    S-173

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