-Cc yu t nh hng n nh lut lambert beer: + Ch ng khi nng cht phn tch tng i thp < 0.

.01 M + Pha long mu khi o loi bt nh hng ca tp

+ Chn di song hp th cc i o mu nhm gim sai lch bc sng so vi bc sng ca nh sang n sc Ngun sang s dng: + n vonfram halogen: to ra bc x kh kin v cn hng ngoi t 380 2500nm + n hydro nng deuterium D2: pht ra ph lin tc trong vng t ngoi 200 400nm B n sc ha l cc b cch t phn x; s lng vch trn mt n v chiu di cng ln (mm) th phn gii cng cao v cc tia tch ra cng n sc H quang hc ca nhng my n gin thng l nhng h mt chm tia cn nhng my hin i l h 2 chm tia u im ca pp quang ph UV- Vis: + Kh nng p dng rng: mt s rt ln cc cht hu c, v c, ha sinh hp thu c nh sng trong vng t ngoi kh kin v nh vy c th phn tch nh lng trc tip chng + nhy cao: gii hn d tm ca ph hp th phn t trong khong 10-4M n 105 M. Khong ny c th c m rng n 10-6M thm ch 10-7M vi nhng th tc b sung + chnh xc cao: sai s tng i v nng trong ph UV-VIS tiu biu nm trong khong t 1% n 5%

*Trong dinh dng vt nui, cht khong ng vai tr khng km phn quan trng - Tham gia cu to xng, cu to t bo - Cn bng cc phn ng axit kim trong c th - To p sut thm thu *S dng phng php o ph hp th phn t da trn bc x vng t ngoi kh kin (UVVIS) cho php nh lng trc tip c hu ht cc cht vi nhy ln ti 10-4 10-5M v chnh xc t 1% - 5%. *UV-1800 l Model my quang ph mi nht t Shimadzu-Nht Bn. Thit k nh gn, phn gii v chnh xc cao hn hn cc Model c. *UV-1800 s dng h quang hc 2 chm tia gip gim thiu thao tc v tng chnh xc so vi h quang hc 1 chm tia ca cc dng my c.