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Lab Syllabus for Molecular Gateway (Bio 2O1L) Spring 2014 Week/ Date Activity Wk 1 (1/8) No Lab this

week

Wk 2 (1/13)

Introduction, Pre-semester assessment & Discussion of Pollard paper MLK No Lab this Week

Wk 3 (1/20) Wk 4 (1/27)

Lab-1: Pipetting Challenge & Introduction to yeast model system (Saccharomyces cerevisiae) Lab-2: Isolation of Yeast Nucleic Acid Lab-3: Restriction Digest of Yeast DNA; Gel Electrophoresis Lab-4: Start cloning project: Ligation of Yeast DNA into Plasmid Vector and Transformation into E.coli Lab-5: Plasmid DNA Isolation & PCR

Wk 5 (2/3) Wk 6 (2/10)
(Exam W eek)

Wk 7 (2/17)

Wk 8 (2/24)

Wk 9 (3/3) Wk 10 (3/10) Wk 11 (3/17)

Lab-6: Restriction Digestion& Gel Electrophoresis Spring Break Lab-7 Analysis of Gels Set up Sequencing Reactions
Switch labs with Bio 202: Bio Sci 0032 Switches with Bio Sci 0034 Bio Sci 0039 Switches with Bio Sci 0040

Wk 12 (3/24)
(Exam W eek)

Lab-8A Introduction to Bioinformatics Tools


Switch labs with Bio 202: Bio Sci 0032 Switches with Bio Sci 0034 Bio Sci 0039 Switches with Bio Sci 0040

Wk 13 (3/31)

Lab-8B Computer Analysis of Sequencing Results from Genomic Inserts


Switch labs with Bio 202: Bio Sci 0032 Switches with Bio Sci 0034 Bio Sci 0039 Switches with Bio Sci 0040

Wk 14 (4/7) Wk 15 (4/14) Wk 16 (4/21) LDOC 4-23

TBL Helicase Activity Student Conferences Student Conferences

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