*

Vibrio parahaemolyticus, V. vulnificus, V.

cholerae Salmonella spp. coliforms, fecal coliforms Escherichia coli
12 2550
2551
(Crassostrea belcheri) (Anadara granosa, Linn.) (Perna viridis, Linn.)
3 3
1.0 x 105, 7.7 x 103, 6.2 x 104 CFU/g 6.0 x 102 CFU/mL

V. parahaemolyticus 5.5 x 102, 7.4
x 102, 7.0 x 102 CFU/g
E.coli
-
E.coli 87.83, 10.39, 60.04 MPN/100 g 7.06
MPN/100 mL V.cholerae, V.vulnificus
Salmonella spp. 8.15, 2.4 6.47



: (Crassostrea belcheri) (Anadara granosa, Linn.)
(Perna viridis, Linn.)
____________
*: .
e-mail: maneeg@fisheries.go.th

Contamination of Pathogenic bacteria in Bivalve Mollusks in Ban Don Bay,

Suratthani
Manee Gannarong, Somnuk Promsorn and Sakuntip Pudkaew
Suratthani Fish Inspection and Research Center
ABSTRACT
A study on contamination of pathogenic bacteria: Vibrio parahaemolyticus, V. vulnificus,
V. cholerae Salmonella spp., indicators bacterial : coliforms, fecal coliforms and Escherichia coli
contaminations in bivalve mollusks harvested from Ban Don Bay, Suratthani was carried out for
12 months from February 2007 through January 2008. During the study, the bacteria
contaminated was isolated from the samples of oyster (Crassostrea belcheri), clam (Anadara
granosa, Linn.) mussel (Perna viridis, Linn.) and sea water that were monthly collected from 3
stations in Bandon Bay and each station had 3 replications. The result showed that total bacteria
in oyster, clam, mussel and seawater at 1.0 x 105, 7.7 x 103, 6.2 x 104 CFU/g and 6.0 x 102 CFU/mL
respectively, which are in the values of staadard criteria of bacterial contamination for fresh body
consumption regulated by the Department of Medical Science (DMS).
The average amount of V.parahaemolyticus contaminated in oyster, clam and mussel
were found about 5.5 x 102, 7.4 x 102, 7.0 x 102 CFU/g respectively, which are lower than the
values of those regulated in the DMS standard criteria of bacterial contamination for fresh body
consumption. The average amount of E.coli in oyster, clam and mussel were found in high
contamination during May June, which are lower than the values of those presented in the
standard criteria for the bivalve aquaculture area of the Department of Fisheries. The average
amount of E.coli in oyster, clam, mussel and seawater were 87.83, 10.39, 60.04 MPN/100 g and
7.06 MPN/100 mL respectively, while the contamination of V. cholerae, V.vulnificus and
Salmonella spp. Were 8.15, 2.4 and 6.47% of the total amount of sample respectively.
The results from this study show a necessity of having prevention measure for safety
consumption of bivalves mollusks. The center for water quality monitoring in the culture area,
the bivalve depuration program after harvest or cooking before consumption of the bivalves
harvested during raining season are the examples of safety program suggested for the
consumption bivalves mollusks cultured in Ban Don Bay, Surathani
Key words: Pathogenic bacteria, oyster (Crassostrea belcheri), clam (Anadara granosa, Linn.),
mussel (Perna viridis, Linn.), Bandon Bay, contamination
_______

*Corresponding author: P.O.Box 9, Suratthani 84130 Tel. 0 7727 4232 e-mail

maneeg@fisheries.go.th