I

i,

HYPOTHESIS (which fruit juice will produce the most vitamin C?). -===-

METHOD
1. Pipette 1cm3of 1% DCPIP solution into a test tube. . 2. Using a pipette/burette, add 1% vitamin C solution drop by drop to the DCPIP solution. Shake the tube gently after each drop. 3. Continue until the blue colour just disappears. This is the END POINT. 4. Record the exact amount of the vitamin C solution that was added to decolourise the DCPIP solution.

5. Repeatthe procedureand averagethe result.

6. Repeat the procedure with the fruit juices provided. NB. The i% vitamin C solution contains img o/vitamin C in i.Gcm3.

CALCULATIONS
...
.>

"

CALCULATION 1 - MASS OF VIT C NEEDED TO DECOLOURISE lcm3 OF - J-t:r CH ~1.(ent

1% DCPIP SOLUTION.

Eg. Suppose it takes 0.6cm3of 1% vitamin C solution to decolourise 1cm3 of DCPIP solution and there is 1mg of vitamin C in 1.0cm3of solution, Therefore, the vitamin C content (mg) needed to decolourise 1cm3of DCPIP solutiGnis:
1.0mg x 0.6cm3 = O.6mg. --';. \; t~ \ / IV' , \j \ :.

( C\ \(' ,l

c:.\

v,! "7 ~e i (V!)

CALCULATIOJ2

- VITAMIN C CONTENT OF FRUIT JUICE (mg/cm\

This value can now be used to work out the vitamin content of the fruit juices in mg/cm3.

Eg. Suppose after 3 tests, the average volume of vitamin C in mangojuice needed to decolourise 1cm3of DCPIP was 1.61cm3,
Therefore, vitami,n C content of the mango juice (mg/cm3) = 0.6 -7-1.61= O.37mg/cm3. ,1-,. ~ ("---, (\ ; \ '-/ ) ( nl I i( {\ " (\>:~( n,~u; \;"'~" j \, '\ \:/ !\ I \\ -'-/ 1- r
' ,',

f'

!,)

'

,~

,if)

1
D.

r,--,
~11\

'!
l'

~,\,1{;'
'\. ",i,."" ,,'

,,1.,//'
'j

-lv)\(

-, L, \ \ j le,', ' "! ,\' ,, -- ,)(Ii"\J:'\"'li'

,

~ ',,"" "; ,,:'\' .1 \0\1"1"" /0 V.I"

11. ~-'
\

cc.". L,~'h

'

\\ ,:/
" ~

','

'\'-,(~p'rP +/ \ -' \
',"

e.)",8on
:

(U , " '\ - \," ' . 1 1(i, -D " '\ f, r

'

r-" rn)"\i'((} \ . '--",.~

- .
\

~\

\ \US'" .
""0 'HJ
I

,
" :.
'IV

'

~,!,.'~'

C",

1 '\'
'\ '

., "
L,

":j

i '('.1)1

->-<-, l' " :..' :>,-1\

J-

\U,'"

" \ , '.(' I F\ \ f \ . '~,,\J-Iv'"

:'t ')

u nP'-'\ (\ \"",,' '\{ .'

,",' l,..\

RISK ASSESSMENT

(identify any risks and list safety precautions)

!

[/

j ,..I

v\ '

if""")

RELIABILITY AND ACCURACY OF RESULTS (how will you ensure that the results are accurate and reliable? How will you ensure that measurements are precise, accurate and repeatable? Include replicates and use of a control).

-::rceQ~('(J

-\hc (Ej~ 1~\eC bples le. j'Ql.

~n

C~I.('(C1~(',

- :t~ ",Q ~jilnj5.a((lIJQlc) -- fl\e(}~ur~ Dc~ J -r

~
\

11\C~(Ult\~,e ~Q\0n\t ~,~e~,

i;

liUJ\ 01J\(ej

,..

0\<1,$5LvOJ(
'.J

VJOSl'\uJ

b~\\.0t~YI

\f'(

-~,~
\(J~tj
I

IDENTIFICATION OF DEPENDENT AND INDEPENDENT VARIABLES

1. DEPENDENT VARIABLE I' \Il = (CJ'(' C'\ \J,t

(
'

2. INDEPENDENT VARIABLE = LJfc JV-'i(~ 3. OTHER VARIABLES = \E\. u\ DCVI\( ' J \Jl-\-C C 'n ~e \il; \ C', \
,~'

'

{O'{Ii. I)
\

..

r \ ~ .\;";'\
~',;
."

" j'-., 1\-0, cid J--<. \;ri',U\",,1

,.,,\

J
I

'V' ", "

I (

QCSID'I"l
I

<:\ vy

DC0'1f)
-~,

I
<.:H.:n
'

~
qD\.i'~

,t).'.:;

r.. ,l) r
Wj L

,-,,'

2f')o ."

IJ(
'),';':'1

[1J\ (:i ; ,/
"'"