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    Pcrblunt Map

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    dv
    The document provides information about the features and components of the pCR-Blunt 3.5 kb plasmid including restriction enzyme sites, primer binding sites, promoters, genes, and origins of replication in 3 sentences or less: The plasmid contains sites for restriction enzymes like MluI, NsiI, HindIII, KpnI, SacI, BamHI, SpeI, EcoRI, PstI, and EcoRV, primer binding sites for M13 Reverse, T7, and M13 Forward, the lac promoter, lacZ-alpha gene, ccdB gene, kanamycin and zeocin resistance genes, and a pUC origin of replication.

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    Pcrblunt Map

    Uploaded by

    dv
    117 views1 page
    The document provides information about the features and components of the pCR-Blunt 3.5 kb plasmid including restriction enzyme sites, primer binding sites, promoters, genes, and origins of replication in 3 sentences or less: The plasmid contains sites for restriction enzymes like MluI, NsiI, HindIII, KpnI, SacI, BamHI, SpeI, EcoRI, PstI, and EcoRV, primer binding sites for M13 Reverse, T7, and M13 Forward, the lac promoter, lacZ-alpha gene, ccdB gene, kanamycin and zeocin resistance genes, and a pUC origin of replication.

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    The document provides information about the features and components of the pCR-Blunt 3.5 kb plasmid including restriction enzyme sites, primer binding sites, promoters, genes, and origins of replication in 3 sentences or less: The plasmid contains sites for restriction enzymes like MluI, NsiI, HindIII, KpnI, SacI, BamHI, SpeI, EcoRI, PstI, and EcoRV, primer binding sites for M13 Reverse, T7, and M13 Forward, the lac promoter, lacZ-alpha gene, ccdB gene, kanamycin and zeocin resistance genes, and a pUC origin of replication.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    117 views1 page

    Pcrblunt Map

    Uploaded by

    dv
    The document provides information about the features and components of the pCR-Blunt 3.5 kb plasmid including restriction enzyme sites, primer binding sites, promoters, genes, and origins of replication in 3 sentences or less: The plasmid contains sites for restriction enzymes like MluI, NsiI, HindIII, KpnI, SacI, BamHI, SpeI, EcoRI, PstI, and EcoRV, primer binding sites for M13 Reverse, T7, and M13 Forward, the lac promoter, lacZ-alpha gene, ccdB gene, kanamycin and zeocin resistance genes, and a pUC origin of replication.

    Copyright:

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    of 1

    M13 Reverse priming site

    Mlu I

    201 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT TTAGGTGACG CGTTAGAATA GTGTGTCCTT TGTCGATACT GGTACTAATG CGGTTCGATA AATCCACTGC GCAATCTTAT
    Nsi I Hind III Kpn I Sac I BamH I Spe I

    CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG CCGCCAGTGT GAGTTCGATA CGTAGTTCGA ACCATGGCTC GAGCCTAGGT GATCATTGCC GGCGGTCACA
    EcoR I EcoR I Pst I EcoR V

    GCTGGAATTC AGG CGACCTTAAG TCC


    Not I

    Blunt PCR Product


    Xho I Nsi I Xba I

    CCTGAATTCT GCAGATA GGACTTAAGA CGTCTAT


    Apa I

    T7 promoter/priming site

    TCCATCACAC TGGCGGCCGC TCGAGCATGC ATCTAGAGGG CCCAATTCGC CCTATAGTGA AGGTAGTGTG ACCGCCGGCG AGCTCGTACG TAGATCTCCC GGGTTAAGCG GGATATCACT
    M13 Forward (-20) priming site

    GTCGTATTAC AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT 470 CAGCATAATG TTAAGTGACC GGCAGCAAAA TGTTGCAGCA CTGACCCTTT TGGGACCGCA

    P lac

    lacZ

    c cd

    Kanamycin

    pCR-Blunt 3.5 kb
    Zeocin

    pUC ori
    Comments for pCR-Blunt 3512 nucleotides

    Lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 LacZ-alpha ORF: bases 217-570 T7 promoter priming site: bases 400-419 M13 Forward (-20) priming site: bases 427-442 Fusion joint: bases 571-579 ccdB lethal gene ORF: bases 580-882 Kanamycin resistance ORF: bases 1231-2025 Zeocin resistance ORF: bases 2231-2605 pUC origin: bases 2673-3386

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