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B.Sc.

Biotechnology (Part-II), Model Test Paper Production of Recombinant Molecules


Time: 3 Hrs. MM: 50

Note: Q.1 is compulsory. Answer five questions in all. Q1. Answer the following accordingly (in short): a) What are reporter genes? Give two examples. b) What are selectable markers? Give two examples. c) Define Cassette. Give examples. d) Explain promoters. Describe the types. e) What are restriction enzymes? Give examples. f) The enzyme used for producing nuclease is________________. g) Define linkers. h) Baculovirus _______________gene is replaced to produce recombinant gene. i) The agrobacterium tumefaciens is attracted towards the plants due to phenolic compounds produced by them to generate_______________in themselves for their survivability. j) What are recombinant prteins? Q2. Q3. Q4. Q5. Q6. What are the transgenic animals? Explain the various methods of their production. What is c-DNA? Explain the method of its production. Define Recombinant DNA Technology. Describe its procedure with well labeled diagram. What are plasmids? Describe the various types. Explain a) Bacteriophage Vectors b) Cosmids (10) (10) (10) (10) (5*2=10) (10*1=10)

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