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    Comments For pEF/myc/nuc/GFP 6232 Nucleotides

    Uploaded by

    dv
    This plasmid vector is 6.2 kb in size and contains genes for GFP expression, a nuclear localization sequence, a myc epitope tag, and antibiotic resistance markers. It uses an EF-1α promoter to drive expression of GFP tagged with a myc epitope and nuclear localization sequence. The vector also contains origins of replication for bacteria and SV40, as well as promoters, polyadenylation sequences, and selection markers.

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    Comments For pEF/myc/nuc/GFP 6232 Nucleotides

    Uploaded by

    dv
    14 views1 page
    This plasmid vector is 6.2 kb in size and contains genes for GFP expression, a nuclear localization sequence, a myc epitope tag, and antibiotic resistance markers. It uses an EF-1α promoter to drive expression of GFP tagged with a myc epitope and nuclear localization sequence. The vector also contains origins of replication for bacteria and SV40, as well as promoters, polyadenylation sequences, and selection markers.

    Original Description:

    yu6t ew

    Original Title

    Pefmycnucgfp Map

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    This plasmid vector is 6.2 kb in size and contains genes for GFP expression, a nuclear localization sequence, a myc epitope tag, and antibiotic resistance markers. It uses an EF-1α promoter to drive expression of GFP tagged with a myc epitope and nuclear localization sequence. The vector also contains origins of replication for bacteria and SV40, as well as promoters, polyadenylation sequences, and selection markers.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    14 views1 page

    Comments For pEF/myc/nuc/GFP 6232 Nucleotides

    Uploaded by

    dv
    This plasmid vector is 6.2 kb in size and contains genes for GFP expression, a nuclear localization sequence, a myc epitope tag, and antibiotic resistance markers. It uses an EF-1α promoter to drive expression of GFP tagged with a myc epitope and nuclear localization sequence. The vector also contains origins of replication for bacteria and SV40, as well as promoters, polyadenylation sequences, and selection markers.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 1

    Not I

    Pst I

    GFP

    3X Nuclear targeting sequence

    myc epitope

    Term

    F-1 PE

    BGH pA

    f1

    or i
    ri 40 o SV

    pEF/myc/nuc/GFP 6.2 kb

    Comments for pEF/myc/nuc/GFP 6232 nucleotides

    EF-1 promoter: bases 6-1192 EF Forward priming site: bases 1133-1153 GFP ORF: bases 1220-1936 Nuclear localization site (3X): bases 1946-1969, 1970-1993, 1994-2017 myc epitope: bases 2030-2059 BGH Reverse priming site: 2095-2112 BGH polyadenylation sequence: bases 2094-2308 f1 origin: bases 2371-2784 SV40 promoter/origin: bases 2849-3156 Neomycin (G418) resistance gene (ORF): bases 3164-3958 SV40 polyadenylation sequence: bases 3975-4213 pUC origin: bases 4396-5069 (Complementary strand) Ampicillin resistance gene (ORF): 5214-6074 (Complementary strand)

    Ampicilli
    n

    Neo m y cin

    p U C or i

    0 V4

    pA

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