##FastQC

0.10.1
>>Basic Statistics
pass
#Measure
Value
Filename
SRR500036.fastq
File type
Conventional base calls
Encoding
Sanger / Illumina 1.9
Total Sequences 14677149
Filtered Sequences
0
Sequence length 202
%GC
52
>>END_MODULE
>>Per base sequence quality
pass
#Base Mean
Median Lower Quartile
centile
1
32.41063812869925
34.0
2
32.63041609784025
34.0
3
32.65272710660633
34.0
4
35.965076460012774
37.0
5
35.79737822379537
37.0
6
35.71590674728451
37.0
7
35.711024532080444
37.0
8
35.70030828194222
37.0
9
37.477363825903794
39.0
10-14 37.66185026805955
39.4
15-19 38.69983456596373
41.0
20-24 38.51896651045785
41.0
25-29 38.4327178255123
41.0
30-34 38.230877522603336
41.0
35-39 37.91183235926813
40.0
40-44 37.4170573726546
40.0
45-49 36.78884626707817
39.4
50-59 35.53230227478103
37.3
60-69 33.50089449252032
35.0
70-79 32.08101390808256
34.8
80-89 31.711899661167166
35.0
90-99 31.15102129166911
35.0
100-109 33.33527210223184
35.3
110-119 38.21674220926694
40.0
120-129 38.58990562812982
41.0
130-139 38.09003933257065
40.3
140-149 37.337629208506364
39.8
150-159 35.688130487739826
37.3
160-169 33.88262835650166
35.1
170-179 32.50533247294825
35.0
180-189 31.688670681206542
35.0
190-199 30.797690505151916
34.8
200-202 28.765951480086496
33.0
35.0
>>END_MODULE
>>Per sequence quality scores pass
#Quality
Count
2
50262.0
3
19900.0
4
20363.0
5
21143.0
6
22157.0
7
20978.0
8
24175.0
9
24453.0
10
19887.0

Upper Quartile 10th Percentile 90th Per

31.0
31.0
31.0
37.0
37.0
37.0
37.0
37.0
39.0
38.8
39.0
39.0
39.0
38.8
38.0
37.6
36.0
35.0
34.2
33.0
33.0
33.0
33.5
38.9
39.0
38.2
37.0
34.9
34.4
33.6
33.0
32.3
29.0

34.0
31.0
34.0
34.0
31.0
34.0
34.0
31.0
34.0
37.0
35.0
37.0
37.0
35.0
37.0
37.0
35.0
37.0
37.0
35.0
37.0
37.0
35.0
37.0
39.0
35.0
39.0
39.4
35.2
39.4
41.0
35.6
41.0
41.0
35.0
41.0
41.0
35.0
41.0
41.0
34.0
41.0
41.0
33.2
41.0
41.0
32.6
41.0
41.0
32.0
41.0
39.9
30.8
41.0
36.4
29.3
38.7
35.0
27.1
35.7
35.0
26.0
35.0
35.0
23.8
35.0
35.6
28.4
35.7
40.0
35.5
40.0
41.0
35.1
41.0
41.0
33.5
41.0
41.0
32.4
41.0
39.8
30.7
40.9
36.8
29.4
39.2
35.0
27.8
36.0
35.0
25.2
35.0
35.0
21.0
35.0
34.666666666666664

2.0

11
21559.0
12
23123.0
13
25256.0
14
27544.0
15
28550.0
16
31122.0
17
34891.0
18
40728.0
19
52694.0
20
57435.0
21
64433.0
22
69653.0
23
75288.0
24
79443.0
25
84312.0
26
93790.0
27
107973.0
28
131201.0
29
163113.0
30
206717.0
31
263653.0
32
346723.0
33
466199.0
34
686694.0
35
1244865.0
36
3248129.0
37
5903680.0
38
869338.0
39
5725.0
>>END_MODULE
>>Per base sequence content
#Base G
A
T
1
25.10890666300168
40.99207936667656
2
25.098710215589133
24.169226779403843
3
30.486523026307943
21.93238838297781
4
28.989921501453253
25.07270005281021
5
27.28927524247245
22.495611991203745
6
23.5870803720312
26.944500039728254
7
22.22085674900038
27.269626888270913
8
24.58289842417108
30.986510825799936
9
24.118291722506267
28.802757075924703
10-14 26.44022212981992
26.62617140980358
15-19 27.34696329185012
25.9169442255394
20-24 27.15500602803285
25.588448678515135
25-29 26.671446634125296
25.746487551708576
30-34 26.079855862438556
26.28305011989281

fail
C
12.537498660396915

21.361515309924844

19.233675116276565

31.498387888730456

21.810070225613966

25.77101836510028

25.1887857583729

20.748592687363644

28.332108299988732

21.88300446633507

28.201906082409227

21.266513505831316

17.75593900105861

32.7535773616701

19.486863041647467

24.943727708381523

20.219262099543542

26.859689102025484

23.099203002147267

23.834403458229232

23.39241103377577

23.343681448834708

23.741196784152578

23.51534850929943

23.694600758611408

23.887465055554724

23.785871951105694

23.85122206656294

35-39 26.130818329099554
23.902659660660817
23.80754185063286
26.158980159606777
40-44 26.546153267612553
24.085112012445844
23.752086033431876
25.616648686509723
45-49 26.526330420986966
23.931226307072322
23.57561135456768
25.966831917373028
50-59 26.83284240132735
23.546127534395534
23.770238729659987
25.850791334617128
60-69 26.74180376356975
24.029882343235382
23.783909111002508
25.444404782192358
70-79 25.893722023062 24.83950667215476
23.596042312886333
25.67072
8991896908
80-89 25.76333875664333
24.682462135771527
23.668558725443685
25.88564038214146
90-99 25.705390303908043
24.821870767454236
23.9272308144276
25.54550811421012
100-109 26.025645140173342
21.88856644948386
24.434262309878616
27.65152610046418
110-119 26.46275863301213
22.587320154961525
23.827444631227817
27.122476580798534
120-129 27.127568587776086
23.297487270538205
23.59479441442786
25.98014972725785
130-139 26.127635744971535
23.662999550266644
23.93876879031509
26.27059591444673
140-149 26.55441152046641
23.853654406409174
23.707089648006928
25.884844425117493
150-159 26.76302153104897
23.283492057090875
24.06460001392398
25.888886397936172
160-169 26.798725022905938
23.76633940667767
23.887414052168484
25.547521518247905
170-179 26.152835091929234
24.546808529906325
23.81777673814449
25.48257964001995
180-189 26.030221452541163
24.5645492074907
23.724289801639532
25.680939538328605
190-199 26.133617087989386
24.48465670091641
24.187647301526326
25.194078909567875
200-202 25.78568678557994
24.319964151003365
24.276078587807813
25.618270475608877
>>END_MODULE
>>Per base GC content fail
#Base %GC
1
66.10098602967824
2
49.26793699499298
3
52.41891140928575
4
54.06262155426346
5
49.784887233676194
6
50.53158041175946
7
49.490483637271296
8
55.56940924997102
9
52.92104879843097
10-14 53.066393539623505
15-19 53.26390751738952
20-24 52.743454706547986
25-29 52.41793418583387
30-34 52.36290598233136
35-39 52.28979848870633
40-44 52.16280195412227
45-49 52.49316233836
50-59 52.68363373594448
60-69 52.18620854576211

70-79 51.56445101495891
80-89 51.64897913878479
90-99 51.25089841811816
100-109 53.67717124063752
110-119 53.585235213810655
120-129 53.107718315033935
130-139 52.39823165941826
140-149 52.439255945583895
150-159 52.65190792898514
160-169 52.34624654115385
170-179 51.63541473194918
180-189 51.711160990869764
190-199 51.327695997557264
200-202 51.40395726118883
>>END_MODULE
>>Per sequence GC content
#GC Content
Count
0
1183.5
1
4206.333333333978
2
3879.666666667313
3
1374.6666666666333
4
1185.333333333343
5
1264.333333333325
6
1276.6666666666556
7
1255.3333333333271
8
1226.3333333333337
9
1210.3333333333374
10
1106.6666666666943
11
1021.0000000000462
12
903.3333333333661
13
804.6666666666882
14
708.0000000000106
15
634.3333333333355
16
593.6666666666642
17
544.6666666666587
18
514.3333333333219
19
494.666666666656
20
465.99999999999096
21
443.9999999999922
22
431.9999999999929
23
451.66666666665844
24
466.6666666666576
25
487.9999999999897
26
558.3333333333269
27
640.0000000000028
28
728.0000000000128
29
884.6666666666973
30
1018.6666666667126
31
1184.3333333333433
32
1483.6666666666085
33
1771.3333333332098
34
2097.3333333331693
35
2546.3333333333735
36
3159.000000000319
37
3941.6666666673414
38
4987.666666666601
39
6676.666666665064
40
10053.666666667072
41
16941.00000000989
42
27998.999999969663

fail

43
46570.33333338607
44
78117.33333334098
45
131763.3333325905
46
217985.00000176654
47
322770.99999952276
48
431095.9999932174
49
534882.9999890263
50
635881.6666674508
51
734431.6666789235
52
781163.3333510305
53
732551.0000120379
54
640179.0000012844
55
527270.9999881402
56
398552.66666177835
57
277685.3333354804
58
185434.0000008192
59
126953.66666596365
60
82624.99999994205
61
54743.0000001122
62
40708.666666676756
63
36813.666666648416
64
37149.9999999842
65
37211.333333317976
66
33196.33333328876
67
26732.333333307604
68
22146.66666665762
69
18865.000000002892
70
14776.333333342329
71
9902.333333333463
72
5826.9999999991705
73
3305.3333333337187
74
2001.3333333331575
75
1297.999999999984
76
760.6666666666832
77
429.6666666666597
78
233.00000000000225
79
111.33333333333283
80
59.00000000000014
81
33.66666666666663
82
10.0
83
4.333333333333333
84
3.3333333333333335
85
3.666666666666667
86
2.333333333333333
87
0.3333333333333333
88
0.3333333333333333
89
0.3333333333333333
90
0.3333333333333333
91
0.0
92
0.0
93
0.0
94
0.0
95
0.0
96
0.0
97
0.0
98
0.0
99
0.0
100
0.0
>>END_MODULE
>>Per base N content
pass

#Base N-Count
1
0.058764818698781356
2
0.00928654468248568
3
0.015697871568926637
4
4.224253633999355E-4
5
0.009252478120921169
6
0.18821775264392288
7
0.15040386930731575
8
0.1745706880811798
9
0.18092750846911754
10-14 0.20171764966070727
15-19 0.2476720785487699
20-24 0.2965112638701154
25-29 0.24368629084572216
30-34 0.2787230680835904
35-39 0.36662297289480406
40-44 0.4777521847056264
45-49 0.47617694689888346
50-59 0.4406209952627721
60-69 0.5164770078984686
70-79 0.6756066862849182
80-89 0.8673448774009176
90-99 0.861441823613019
100-109 0.41162421939029165
110-119 0.3604194520339066
120-129 0.41023839166584736
130-139 0.39545895459669994
140-149 0.34552623264913374
150-159 0.2859090685800083
160-169 0.2718361719977088
170-179 0.3128775213769377
180-189 0.4124227395933638
190-199 0.39160875180867893
200-202 0.39045048871548554
>>END_MODULE
>>Sequence Length Distribution pass
#Length Count
202
1.4677149E7
>>END_MODULE
>>Sequence Duplication Levels fail
#Total Duplicate Percentage
87.95407482990281
#Duplication Level
Relative count
1
100.0
2
24.477702245791367
3
15.083460867708297
4
11.803751290173327
5
9.789301348898459
6
8.57030999750863
7
7.5791009716339826
8
6.872619852653308
9
6.125209097056625
10++
165.60842794604406
>>END_MODULE
>>Overrepresented sequences
warn
#Sequence
Count Percentage
Possible Source
CGGGAACGTATTCACCGCGCCGTGGCTGATGCGCGATTACTAGCGAATCC
44716
414
No Hit
CATCGGGAGTGACGTGGAGACGGAGCAGTGACAGTGCCGCGGACTGACGG
18517
976
No Hit
CCGGGAACGTATTCACCGCGCCGTGGCTGATGCGCGATTACTAGCGAATC
18286

0.30466407338373
0.12616210409800
0.12458822895372

937
No Hit
TTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAATGCTT
422
No Hit
>>END_MODULE
>>Kmer Content warn
#Sequence
Count Obs/Exp Overall Obs/Exp Max
TTCAC 5894990 2.221943
8.9802685
2
CCTTA 5857670 2.2078762
6.141124
6
TTTCA 4989365 2.0475376
8.228949
1
GTTAC 4834405 1.7987375
5.0786643
2
ATCCA 4411600 1.6750307
5.4927306
6
TATTC 4028800 1.6533406
8.844449
9
TTAGC 4314890 1.6054416
5.1855564
2
AGTTT 3872975 1.5689393
5.0088944
4
TCACC 4354455 1.5074637
5.9676156
8
TTTGA 3603100 1.4596133
5.0819774
2
GTTTG 3916380 1.4279327
5.0595717
1
GTATT 3475030 1.4077321
8.682295
8
GGGAA 3913255 1.3126618
7.555833
2
GGAAC 3849650 1.3081603
8.036502
3
TCACT 3445400 1.298642
5.60745 3
ATCGG 3759965 1.2683725
5.238323
2
CACTC 3625940 1.25526 6.4743953
4
CATCG 3576515 1.2222162
5.4990788
1
ACTCT 3234435 1.2191252
5.2162776
5
CGTTA 3274860 1.2184775
5.04318 1
CGGGA 3856910 1.179616
7.9985204
1
CGTAT 3008230 1.1192722
7.2666616
7
GAACG 2781880 0.9453184
7.1113405
4
ACGTA 2515475 0.9428042
8.8096695
6
AACGT 1985120 0.7440263
7.285492
5
>>END_MODULE

15476

0.10544282135447

Max Obs/Exp Position