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IS 4162-1 (1985): Specification for Graduated Pipettes,
Part 1: General Requirements [CHD 10: Glassware]


IS : 4162 ('art 1) -1985
Indian Standard
SPECIFICATION FOR
GRADUATED PIPETTES
PART I GENERAL REQUIREMENTS
( First Revision)
Laboratoryware and Related Apparatus Sectional Committee,
. CDC 33 .
1l.fIrum/i,.,
Bhabha Atomic Raearch Centre, Trombay,
Bombay
Mnnlns
SIdI S. V. Gvt.AVA!C& (.oUuru" to .
Dr M. SaDkar DaII )
Da G. S.BAJWA Defeace Sciencc Laboratory, MiDiatry of Dcl'euce
( R&D), Ncw Delhi .
Da N. K. B...aJU Indian Agricultural ReRarCh Inatitute (ICAR),
NewDc1hi
Da K. S. RAM (.um.,,)
SBaI N. G. BAIAK Directorate General of Technieal Devdopmeat,
New Delhi .
Sam I. It. KAPooa (A.lImwJU)
SDJ M. L. BJr.uoAJq National Test House, Calcutta
DaB. B. PAL (.."..,.)
SBaJ S. K. CRAItLUAaTI Devdopment ComJniIaiOI1er, Small Scalc Indwtna,
NewDclhi
SBaJ I . R. MUJlDAaGl ( Alu",.,,)
LT-Ccn. M. CRAjlwoaTY Dira:tor-GeDeral Armed Forcea Medical Serriccs,
Miniatry of DcfeDcc (DGAFMS ), New Delhi
Da A. V. DlvuAa Bio-Sc:icace Corporation, Bombay
!sal R. K. GADODIA Swutik'I"hermometer Co. Delhi
SIIaI D. S. GAIlOIlIA (.4llirMt.)
SIDI V_A l'ItAumGUPTA Hicb'I'bamclmeten ( IDdia) Ltd, Aliprh
S8aI S. C. ItAPILA(A.ltmtta14)
Da R. C. KBDA Puahpak Entcrprisel, BoIabay
SIIaI D. C. KRuA ( .f1InuU )
S_MOIIINDPNAT. National Pbyaica1 Laboratory (CSIR), New Delhi
S_B. G.MATBoa ( .4l,.,..)
(CMIiaM.. ,.,2)
Copyrlgltt 1986
INDIAN STANDARDS INSTlT'UnON
TIIia pub1icat!0D iI protoc;ted under 1M 1ttdUI1t Co""'" An (XIV.of 1"') aad
rq:cuClucdoa to wholll or to part b1UlJ""tlICIIPl withwritt.- JlII'lII1IIioD orlM
IbaU lie deemed to be iDIrin..... of CClP1rIIb& __...... Act.

IS: 4161(Part 1 )-1985
(c"'"jrI_I-I' 1)
MI1fII1J"s JU;UI/tIiIII
S... P. R.. llAo BoroIil Glua Worb Ltd. Bombay
SHaI U. T. 'fi1lll1l {AltmulU)
SPI H. N RAVI Modern lnatrllmeD... Banplorc
Da F. L. SA1DllAJfA . Haft'tiDe lnatitute. Bombay
SRIlI A. B. C.URODLUL (.fIt__)
SRIlIBa.uro K. SAMPAT The Scieuliic Indiu Gu Co Ltd, Calcutta
SHU D. R. S (AlIIrM")
SHU S. K. SUGAJ. Tano.na ProclueuLtd. Calcutta
SHa' J. C. SRAJIDILYA Top SyriDge M.r.Dufa.c:turiq Co, Bombay
SBUJ. K. WAD(.&II'UU)
S.a. G. S. SJIVIU.A MirWtry oCDcfcnco( 001). NaIFDeJhj
SIIU K. K. AGNIBOTU ( .4l11tMU)
Da B. C. SJIfRA Ceutnl Glau &: Ceramic a-arch laiatitute
( CSrR ). Calcutta
SIIU R. S.. .
SBU G. P. SarVAaTAYA India MeteolCllical Department, New Delhi
8IIU S. GoPI NA'I'B (.41"""') .
SIIIlI SAm. CirAJID... Dim:cor GeIleral, lSI C&-4I.im M-w}
Director ( Chem )
SUrIItIry
....M. M. M.u.aoraA
Deptdf Direct (Chcm.). lSI
Volumetric Glauware Subcommittee. CDC 33 : J
c.-
Sau P. 8oroIil Glau Wor" Ltd, Bcmtbay
MlItI1Hrs
S... U. T. T__
Sbri P. R. Rao)
Sau S. V. GULAVAQ BhabhaAtomie 1leIearc:h Centie, Bombay
Sau K . M. MArnlAL MiDiatry or Defence (001)
S... R.. K. GtJIOYA( .4lJnuu) .
SIIIlI MoBDlllaa NA'I'B NadosWPh}'lica1 Laboratory ( CSIR). NewDeIhl
SIIIlI BaAJIU K. SAIII'AT The Seicntilie Illdiaa Glaa CO Ltd. Calcutta
SBu D. R. Sa (.4l1m1ort,)
SIIIlIJ. C. SIWfDJLYA Top SyriDge MaIlufactt&riq Co, Bombay
SsuJ. It. WAD( .411mM11 )
n..B. C. Snou. Qcntral Glul a: Ceramic Raearclt Inltitute
{CSIR}. Ca1catta
SBu S. VAIDYAlfATBU Vendl Glall Worb Pvt Ltd.lIaDplore
2

IS: 4161 (Put 1)-1985
Indian Standard
SPECIFICATION FOR
GRADUATED PIPETtES
PART 1 GENERAL REQUIREMENTS
( First Revision)
o. FOREWORD
0.1 This Indian Standard (Firat Revision) was adopted by the Indian
Standards Institution on 30 October 1985, after the draft finalized by the
Laboratoryware and Related Apparatus Sectional Committee had been
approved by the Chemical Division Council.
0.% This standard was originally published as IS: 4162-1967. Subsequently,
International Organization for Standardization brought out the following
standardsz
ISO 835/1-1981 LaboratoIY g1assware-Graduated pipettes-Part 1 z
General requirements
ISO 835/2-1981 Laboratory glasswaro-Graduated pipettes-Part 2 ~
Pipettes for which no waiting time is specified.
ISO 835/3-1981 Laboratory glassware-Graduated pipctte.-Part 3 I
Pipettes for which a waiting time of 15 s is specified.
ISO 835/4-1981 Laboratory glassware-Graduated pipettes-Part 4 :
Blow-out pipettes.
The committee responsible for the preparation of this standard decided
to revise IS : 4162-1967 to align with these ISO standards. Accordinglythis
standard is now being revised in four parts : -Part I-General requirements,
Part 2-Pipettes for which no waiting time is specified; Part 3-Pipcttes for
~ i waiting time of IS seconds is specified; and Part 4-Blow-out
pipettes.
8.3 In this revisionthe following have been included:
a) Method of setting of meniscus,
b) Procedure for measurement of capacity,
.Specificatioo Cor lfIlduated pipettcl.
3

IS : 4162 (Part 1 ) -198!
c) Pipettes having OS-ml capacity with Class A accuracy for Type 4
only.
d) Pipettes for which a waiting time of 15s has been .specified.
e) Pipettes known as pipettes. and
f) Table for dimensions haw been modified to bring in line with ISO
Standards.
0.4 This standard contains 13.2 which calls for agreement between the
purchaser and-the supplier.
o5 For the purpose of decldin' whether a particu1arrequirement of this
standard is complied with. the fl;nal value. observed or c:alcuIated, express-
ing the result of a test or analysIS. shan be rounded off in accordance with
IS : The number of signifiC811t places retained in the rounded
off value should be the same as that of the specified values in this
standard.
1. SCOPE
1.1 This standard ( Part 1 ) specifies the general requirements for graduated
pipeUel. suitable for general laboratory purposes.
1.1.1 The requirements specified are in conformity with IS: 8729-I'77t.
1.1.t.l Limits of .error for class A l'ipettes are shown in relation to
capacity in Appendil: A and in relatton to diameter at meniscus in
AppendixB.
2. TERMINOLOGY
2.1 Por the purpose of this standard. the definitions given in IS:
in addition to the foUowinS shall apply.
2.1.1 Reference Tempnature - The __perature at which the pipette is
intended to deliver its nominal volume (nominal capacity ). is27C.
2.1.2 Unit of Volume - The unit of volume is the cubic centimetre
(em' ). for which the Dame JDillilitre (D1l) is used.
2.1.3 Capacity
2.1.3.1 Capacity for Type 1 pipette - The capacity corresponding to
any graduation line of a pipette is the volume of water at 27*Cexpressed in
*R.ules for roUDdin, off Dumuica1 values ( rlVi*).
tPriDciples of CODJtrw:OOIl and aqjUltmeut of volumetric glUIWaN.
tGloeary of terms related to 11&11 and glUlWare (jirlt mIirioII).
4

IS : 416% (Part 1 ) 1985
millilitres, delivered by the pipette at 27C when emptied the line
to that graduation line. the outftow being unrestricted until makmg the
final setting of the meniscus on the graduation line, and no allowed
for drainage of the liquid adhering to the wall before makmg final
setting.
1.1.3.% Capacity lor Type 2 pipette - The capacity corresponding to
any graduation line of a pipette is the volume of water at 27C. expressed
in millilitres delivered by the pipette at 27C. when emptied from that gra-
duation line to the jet. the outflow being restricted until it is sure that the
meniscus has come to rest in the jet before removing the pipette from the
receiving vessel.
1.1.:U Capacity for Ty,. 3 pipette - The capacity corresponding to
any graduation line of pipette is the volume of water at 27OC. expressed in
millilitrcs. delivered by the pipette at noc. when emptied from the zero
line to that graduation line or in the case of total capacity delivering, to
the jet. the outftow being unrestricted until making the final setting of the
meniscus on the graduation line. and the -period aUowed for draining of
liquid adhering to the wall before making the final setting.
2.1.3.4 Capacity for 4 ( /S s waittn8 time) pipette - The capacity
corresponding to any graduation line is the volume of water at 27C.
expressed in millilitres, delivered by the pipette at 27C when emptied frOlJl
the zero line to that-graduation line. the outilowbeing interrupted whc:nthe
meniscus has come to a few mil1imetres above the graduation line. final
setting is made to the graduation line after waiting time of 15s,
In the caseof delivery of total capacity to the jet. the outflow shall be
unrestricted and a time of IS s shall be observed before removing
the pipette from the receiving veSsel.
2.1.3.5 for Type S ( blow-out) pipette - The capacity
correspondiDg to any araduation line for a graduated blow-out pipette is
the volume of water at 27C. expressed in millilitres, delivered by the
pipette at 27C. when emptied from the graduation line to the jet outflow
being unrestricted until it is sure that the meniscus has come-to reU in the
jet. with delivery being completed by expelling the last drop by
bloWJng.
2.104 Delirery Time - delivery time is.the time occupied by the free
descent of the water menISCUS from the hIghest graduation line to the
10000est line, in the case of I pipettes or to the point at
whichthe mernaeus appean to come to rest JD the Jet for Type 2 T)'PCl 3
lJP!'4 and:X:ype s In case, the pipette shall remam in
vertical poaition and WIth the reeemns vessel slightly inclined so that the
tip of tile jet is !It contact with the inside of the vessel. but without move-
ment of one aJ81Dst the other.
,

IS : 4162 ( Part 1 ) 1915
2.1.5 Deli"ery Jel- The lower end of the pipette which has been
Jradually tapered without any sudden constriction at the orifice and could
live rise to turbulent outfiow is called delivery jet.
2.1.6 Wailing Time- The waiting time is defined as the period of time
to be observed after the meniscus appeared to have come to reat in the jet,
and before the tip of the jet shall be removed from the receivin. vessel.
3. TYPES OF PIPEITES
3.1 The following types of graduated pipettes are specified.
a) Type 1- Graduated pipettes adjusted for delivery of a liquid
from zero lino ar the top to any graduation line, nominal .
capacity represented by the lowest graduation line, no waitin,
aimeis specified for accuracy. Class A and Class B (lee Part 2
of this standard ).
b) Type 2 - Graduated pipette adjusted for delivery of a liquid
from any graduation line down to the jet, nominal capacity
.represented by the highest graduation line. no waiting time is
specified for accuracy Class A and Class B (see Part 2 of this
standard ).
c:) Type 3 - Graduated pipette adjusted for delivery of a liquid
from zero line at the top to any araduation line, nominal
capacity obtained by delivery down to the jet, no waiting time is
specified for accuracy Class B only ( see Part 2 oftbia standard ).
d) Type 4- Graduated pipettes adjusted for deIiveryof a liquid
from zero line at the top to any graduated line, nominal c:apaclty
obtained by delivery down to the Jet. 15 s waiting time is specified
for ac:c:uracy ClaasA only (see Part 3 of this standard ).
e) Type S - Graduated pipettes adjusted for delivery of a liquid
from any graduation line down to the nominal capacity represen-
ted by the highest gTaduation line, the last drop in the jet
to be blown out lor accuracy Class B only (see Part 4 of this
standard).
4. ACCURACY
4.1 The graduated pipettes shall have the foDowing two c:lassea of ac:curacy
(lee Table 1): .
a) Class A - For the higher grade suitable for precision work.
b) Class 11- For the lower grade suitable for JCDeral purposes.
6

"
IS ; 4161 ( Part I ) - 1985
.s, NOMINAL CAPACITY
5.1 The graduated pipettes shall be of 0" ml, I mI, 2 mI, Sml, 10mI and
2.5 m1 nominal capacities respectively.
5.1 Procenre for MelSaremeat of Capadty
5.2.1 The dry and clean pipette shall be held in a ver?cal position and
filled with water to few millimetres above the graduation line or above tho
zero line, depending upon .the type of pipette the menis.cusshall then
be set to the line. Any drop adhering to the jet of the pipette shall be
removed by bringins the surface of a glaslvessel into contact with tho tip
oltho pipette.
5.1.2 Delivery shall then be made into another glass vessel slightly
inclined, so that the tip of tho jet is in contact with the inside of the
vessel, but without movement or one against tho other throughout the
delivery time and waiting period in for the Typo 4 pipette only ( see Part 2
of this standard). In case of S pipettes. the liquid remaining in the
tip of the jet shall be blown out m tho glass vessel.
5.1.3 To ensure that delivery is complete in the case of Type I. Type 2
and . Type 3 pipettes, a waiting time of approximately 3 s shall be observed
before removing the pipettes from the receiving vessel.
Nors - The waitiDI period or! s it apeciSedoaly rOl' the purpole or definitiOll. 1JI
use, it is UDDecaaarr to adhere; dOle to Ih1Iperiod, it it .umcient to be certain that
the mcIliacul hal come to rat ill tile jet before retDOriJls thc pipette Crom COQtac:C with
the receiviDt .esIC:l.
5.1.4 Thewater delivered as described in 5.2.1 to 5.%.3 above is cOllected
in a previous weight glass container -and is weighed again to determine
the mass. of the water delivered. The temperature of water, air and
boremetric pressure are noted and that the volume of water delivered by the
pipette at 27C is calculated by applyinl appropriate corrections as liven
in IS: 1729-1977-.
,. UMITS 0' VOLUMETRIC ERROR
&rors in the volume shall not exceed tile limits as shown
m:r
ablc
I . These limits represent the maximum permissible error at any
PO!Dt and the maximum permitted diff'crence bctW'CCll the errors at any two
poJDtl.
.PriDcipIa of CUiiftlldioa aDdMljlutmem of vohamctric
7

IS : 416'1. ( Part 1 ). 1985
TABLE I CAPACITIES, SUB-DIVISION AND UMrn O' ERROR
(Cla.s. 6.1 )
0'01
0'02
0'05
0'1
Class B
ml
(5)
SILUI.Ur
SCALa DIvmON
NOIlUfAL
CAPACITY
SL
No.
'Class A
ml ml m1
(1) (2) (') (4)
i) 0'5 0'01 0-005
ii) 1 0'01 0'006
iii) 2 0'02 0'01
iv) 5 0'05 0'03
v) 10 0'1 0'05
vi) 25 0-1 0'1
vii) 25 0'2 0'1 0'2
NoD 1- Ifpipettca are required of capacitica and/or lub-divilioDI other than thOle
listed in this table, "it is recommended that they coDform to the essential requirement of
this part of specificatiOD.
NOTa 2 - The limitl ofvolumetric error shall Dot exceed the lDlallat lCale diYiliOll.
7. CONSTRUCTION
7.1 Materi..
1.1.1 Graduated pipettes shall be constructed from glass of suitable
chemical and thermal properties, shall be as free as Car as possible from
visible defects and shall be reasonably free from internal stress. When
graded according to the metbod prescribed in IS : 2303-1963* the pipettes
shall confirm to Type 1 of the glass.
Non- Amber or other coloured g1asI IDay beased tor spec:iaI purposes.
7.1. DiaaeasiODS
7.2.1 Graduated pipettes shall comply with dimensional requirements
given in Table 2.
7.3 FiDisII- The graduated pipettes shall be ofrcauIar shape and smoothly
finished. They shall be symmetrical about the axis.
7.3.1 Top of i p ~ t t - The top of pipette shall be finished square with
the axis of the pipette and shall be frco from any blemishes which might
interfere with the required accurate control by the finsa' in settin. tho
meniscus. The end may be lightlyfire-polilhed or smoothly Jfound with a
slight bevel on tho outside.
-Method of gradiur "lISfor alblinity.
8

TABLE 2 DDDNlIONS
( C1QU# 702.1 )
DnOllfl101l1 NOMINAL CAPACITY, m1
(mm> r ~ ______~
0'5 1 2 5 10 25 25-
D!ttance (rom zero line to lawen
MQJt
- 220 220 220 220 220
gr.duation line (or Type 1 pipettea Min
- 160 160 180 180 180
Eaential Distance from highest to 10wClt MQJt 220 220 220 220 220 220 290
dimentiOIlI li3:ed pu.tion lines for each Milt 140 140 140 160 160 160 250
Cmm) o apipette
Diataace from JUPat ....du.tion Min 100 100 100 100 100 100 100
line to top of pipette
'0
Exteraal cnalDeteJ' of auction tube MQJt
- - - 8'3 8'S SoS OoS
Mill - - - 6'8 68 6'8 6'8
Leqth altube oIuaiform bore bdow Min 10 10 10 10 10 10 10
lowest ....duation line
OYenllleqth S60 360 360 360 360 360 450
fi.l
..
..
llec"'I'''C''o:led Leqth aI tapered portion Conning jet 20 20 20 25 25 30 SO
e
namlaal
climenJiOIll F.stemal diameta of jet .t top of 25 25 25 3 S 3 3
-
<mm) bcvd
~
Wall tbicbell
::l
2 2 15 I 1 I I ...
'-'
Valid only for til, 25/01 ml pipette <SII Part' oflhil atandaJd ).
,
...
\0
00
Ut

IS : 4161(Part 1 ) - 1985
7.3.1 let - The lower end of the graduated pipette shall
terminate in d.elivery jet a smooth and gradual taper without any
sudden constncnon at the orifice which could give rise to turbulent outflow.
. The end of the jet shall be Jinished by one of the methods listed below
(In the order of preference) :
a) Smoothly ground square with the axis sliptly bevelled on the
outside and fire-polished; ,
b) Smoothly ground square with the axis, slightly bevelled on the
outside; and
c) Cut square with the axis and fire-polishect.
8. GRADUATION AND FIGURING
&1 Gradllltioa Uae.
8.1.1 Graduation lines shall be clean permanent and uniform lines of
thickness not exceeding 03 mm.
8.1.1 All graduation lines shall lie in planes at right angles to the
longitudinal fUil of the pipette.
8.2 S,acia, of Grad....oD LIDes
8.2.1 There shall be no evident irregularity in the spacing of the
pduation Jines.
8.2.2 The limits on the spacing of graduation lines shall besuch thit the
scale lengths are within the limits allowed in Table 2.
&3 L.ltJa 01Gn...tioD LiHI
8.3.1 Graduation Pattern 1
a) The length of the short linea shan beapproximately, but not
less than SO percent of the circumference of the pipcUel;
b) The length of the medium lines shall be approximately 65 percent
of the circumference of the pipettes and shall extend symmetri-
calIyat each end beyond the end of the short linea; and
c) The Jong lines shall extend compIetcJy round the c:in:umference of
the pipettes, but a saP. not exceeding 10 pcm:Dt 01tho c:ircam.
ference, may be permitted.
8.3.2 Graduation Pattern 2 .
a) The length of the short JiDea shaI! bcnot leas than 10 percent and
not more than 20 perceotof tho c:ircumfCll'CDce oftho pipettes;
10

IS : 416% ( Put 1 ) 198!
b}The -JIh of lhe medium wa ,han be approximately ' :5 times
dw _,til or the short liJIII aDd ahaJ1 ellald Symmctllcally at
~ end bc,ond the CDda or the Ihort liDa; and
c) The 10D' lines ,haJJ extend completely round the circumference
of the pipetta but PP. DOt elalCldinl 10 percent of the
cucumlerenc:e. may be pmnitteel.
U-J GroJ-'iDII PGII",. J
.) n. Imltb 0( the sbort liDa IhaJI be Dot ICII than 10 percent
aDd DOt more tban 20 percentof the circumference or the pipettes;
b) ne !eDith 0( tbe mcdilUD lina ,baD be .ppfosimately I'S times
the IenJtb or the sIkm linea and ahan extend lymJDetric:aUy at
each CIId bcyODd dw ends of tho ,bon tiDeS; and
e) The Ienatb of the lon, liDa ahaJJ benot leu than twice tho lenath
01 the.nort liDa and sball uteud ,ymmetrically at each end
beyond tbe end oftbe short and medium 1iDcs.
IA ~ 1M Se'.I.tA "G....... U.. (1ft 111.1)
Ul OIl pipettes ia which the YOlume equiftlent of the smallest seale
dirision is 0-0I ml or 0-1 mJ:
.} Emy tCDtb ",.dllation liDe shaD be a long line;
b) t'berc ahaU be medium line midway between two CODsecutive
lOll' lines; aDd
c) t'bere shall be four short lines between coDJecutive medium and
kJq rmea.
.....2 OIl pipettes in which the YOIume equivalent of the smallest ecale
4iwisioe II 01U .. or 0'2ml:
a) 1!wrJ 6f\b lfaduatiOD line abaIl be a Jon, line; ad
b) n.eahaJl be(our abort liDes bttwecotwoCOIlIClc:Utivc malinca.
c ~ ~ o ~ r : ~ ill -bich tbe YOla&me equinJaat of the smallest ae
a) 2wry lath padaatioa liDe shall be.lon,liDe;
b) 1'1IIen ~ be rour medham lines equaUJ spICed betweentwo
COIaaeC\a1Ye 1oa,1iDa; aad
c) ~ e ,baD one short tiDe belwem two CODIeCUtive medium
.... Clf bet CODIClCUItfe mcdiua aJld loa, linea.
U ......... GIII u..(.. J1I.%)
U1 OIl pipettes pachaated aceontin\: ttan J
*'" "..boa 1_ lUll lie 011 an pa ~ :1 ~ cadi or the
_,nlllty Yc:mc:aJ liDe downthe
11

IS :4162( Part 1 ).1985
o
0'01 0-02
0,'
O-Qt
0'2
1 2 3
A GRADUATION PATTERN
0
0 0
-
-
-
-
-
-
-
1 2
3'
B GRADUATION PATTERN II
0 0 0
-
--
- -
1 2 3
C GRADUATION PATTERN lIJ
FIG. I LEN<mI AND or Ol,ADUA11O'N lJ.-
GRADUATION
PAHUN J
fJo. 2 ,...... or OUDUA1'ICII'f l.DGI
12

IS : 4162 ( PII1 1) -1915
centre of the front of the pipette, the lines themselves extending
to the left when the pipette is viewed from the front In the posmon of
norma) use.
8.5.1 On pipettes graduated according to pattern 2 or 3, the mid-points
of the short and medium graduation lines shall lie on an vertical
line down the centre of the front of the pipette, when the pipette IS viewed
from the front in the position of normal use.
8.6 FJpriag of Grad.doD LiaeI- Details are given in Table 3.
TDLB 3 fiGURING OF GJUDUATJON UNBS
SL
No.
(I)
i)
ii)
jji)
iv)
v)
vi)
vii)
NOIlllUL
CAPAClI'rY
(mt )
(2)
0'5
1
2
5
10
25
25
SIIALLUT SCAU
DlVDtOlll
(ml )
(3)
0'01
0'01
0'02
0'05
0'1
0'1
0'2
FIOUaaDAT
EVERY
(mt)
(4)
0'1
0'1
0'2
0'5
1'0
1'0
2'0
,. PERMANANCY AND VISIBILITY os GRADUATION LINES,
FIGURES INSCRJPrIONS AND MAIlIINGS
'.1 AIl figures inscriptions and markings shall be of such size and form as
to be clearly legible under normal condition of use. .
'.1 AU araduation Jines. figures inscriptions and markings shall be clearly
visible and permanent.
,.3 If enamel colours are used for above purpose. the requirements of
IS : shan becomplied with.
':4If any other method is for above purposes, the araduated
pIpettes shall be completely l1Dmcraed In 0'1 N hYdrochloric solution and
autoclave at 98'1 tN/rn
l
at 120"C for 30 minutes. The marleing shall not
show appreciable reduction in intensity.
*Method. for .-iDs the chelQical resistuu:e or enamel. used Cor colour coding and
colour markmg.
13

IS ; 4162 ( Part 1 ) - 1985
10. SETTING OF THE MENISCUS
10.1 The meniscus is set so that the plane of the upper edge of the
graduation line is horizontally tangential of the lowest point of the menis-
cus, the line to sight being in the same plane.
11. DEUVERY TIME
11.1 The delivery time shall be within the limits specified for the particular
pipettes.
11.1 The delivery time is determined with the pipetto in a vertical
position and with the receivin g vessel slightly inclined ao that the tip of the
jet is in contact with the inside of the vessel, but without movement of one
against the other.
NOTIl - It is important that glall receiving vessel iI used. Capillary dl'ectl
inftuenemg the delivny time depend eowiderably oa the material oa which the liquid
runs down.
u. COLOUR CODING
11.1 If colour coding is used on these graduated pipettes, it shall comply
with the requirements of IS : and IS: 11469-198St.
13. MARKING AND PACKING
13.1 Each pipette shall be market permanently and legibly on its surface
with the following information:
a) Nominal capacity followed by 'ml';
b) Letter 21C to indicate the reference temperature;
c) Letter 'N or 'B' to indicate the class of accuracy for which the
pepette has been adjusted;
d) The waiting time, if specified;
e) The delivery time;
f) Type of pipette;
g) Asmall white ring (etched, sand-blasted 0 r enamelled) close to
the top of the pipette, in the case of blow-out pipettes. Addi-
tionally, these pipettes may have an inscription that the instru-
ment is a blow-out pipette, for example, 'blow-out';
h) Maker's name or 'reeognized trade-mark, if any; and
J) ''The letter 'Ex' to indicate that the pipette has been adjusted for
delivery".
SpecifiClLtion -colour ccx1ing of pipettes.
t Method of --.j1Jl chemU:a1 residaoc:e ore:aameIs used for coIoar codinr.
14

IS : "16% (Part 1). 1985
13.1.1 The pipette mayalso be marked with the lSI Certification Mark.
NOTJI - The we of the lSI Certification Mark ialO"emed by-the proviaionJ of the
Indian Standanb Institution (Certificatioa Mub) Ad, and the Rules and Regulation
made theftuDder. The lSI Mu& OD producta covered by an Indian Standard conveye
the.-.nee that they have been produced to comply with the requirementl of that
ltandard under a well-defined IYItemof inlpection. tc:Itinl and quality control which
iI deviled and I U ~ by lSI and operated by the producer. lSI marked pro-
ductl are aIIo COIltlnUOUlly checked by lSI for conformity to that ltandard u a further
I&fesuard. Detaill of conditiom under which a licence for the lIIe of the lSI CertifiCA-
tion Mark may begranted to manufacturen or proc:eaors, m"y be obtained from the
Indian Standard. In.titution.
13.% Tho pipettes shall be packed as agreed to between the purchaser and
the supplier.
1'" SAMPLING
14.1 Representative samples of the pipette shaD be draq as prescribed in
AppendixC .
P P E N I ~ A
(C/Quse 1.1.1.1)
ULATIONSIDP BETWEEN VOLUMETRIC CAPACITY AND
LIMITS OF EUO. FOR CLASS A PIPETTES AS
REQUIRED BY 3.4 OF IS: 7829-1977"
2S 10
/
-:
I'
V
V
-:
./
/'
/'
6
5
0.5
10
30
2 5
CAPACITY OF PIPETTE
1
ml
Principla of CDIIItnIctioll and adjustment of YOlumetricsi-ware.
"3. 100
-It

o
ffi SO
15

(S : 4161 (Part 1 ) 1985
10
5
u,
o
!:e
!e
... -
u.1II:

t-III: 0.9
Zw

0.7
>t-
-w
52
w3
111:0

w
z
::i
1.8 206 3.8 5.9 8010 to"' 0.3
MENISCUS DIAMETER, mm
APPENDIX C
( C/llUU 14.1)
SAMPLING OF GRADUATED PIPEIIES
c-i, SCALE OF SAMPLING
C-l.l Lot -Ina single consignment of the pipettes ofthe same type, class
and capacity shall constitute a lot .
C-t.1 Sampleshan be tested from lot the cOnformity of
the pipettes to the requirements of this specificatJOD and shall be m accord-
ance with Table 4.
PrillCiples ofconstruetion and adjustment oIYGl.-etric pl.laW&tt.
16

IS : 4161 ( Part 1 ) - 1985
TABLE 4 NUMBER -OF PI "I"I'ES TO BB SELECTED
( ClauR 01.2 )
Lor Sua SAllPUI Srza POa -SAMPUI Srza POIt
DEITIlucmva TBST . NOIfDaITllUarlva
Tan
(I) (2) (3)
Up to 25 1 3
26 to 50 2 3
C-l.3Pipettes shall be selected from the lot at random and in order to
ensure the randomness of selection. the method given in IS : 4905-1968*
may befollowed.
C-Z. CRITERIA FOR CONFORMITY
C-Z.l The lot shall bedeclared as conforming to the specificationif all the
pipettes selected. pass destructive and non-destructive tests.
-Methods for random lampling.
17

AMENDMENT NO. I JULY 2007
TO
IS 4162 (PART 1): 1985 SPECIFICATION FOR
GRADUATED PIPETTES
PART 1 GENERAL REQUIREMENTS
( First Revision)
(Page 8, clause 7.1.1) - Substitute the following for the existing clause:
'7.1.1 Graduated pipettes shall. be constructed from glass of suitable chemical
and thermal properties, shall be as free as far as possible from visible defects
and shall be reasonably free from internal stress. When graded according to the
method prescribed in IS 2303 (Part l/Sec 1) : 1994*, the pipettes shall conform
to Class HGB 1 of the glass.'
NOTE - Amber or other coloured glass may be used for special purposes,'
(Page 8,/ootnote marked *) - Substitute the following for the existing:
'*Grading glass for alkalinity: Part I Hydrolytic resistance, Section 1 Hydrolytic resistance
of glass grains at 9S'C - Method of test and c1assification (first revision). '
(Page 15, clause 13.1.1) - Substitute the following for the existing
clause:
'13.1.1 BIS Certification Mark
The pipette may also be marked with the Standard Mark.
13.1.1.1 The use of the Standard Mark is governed by the provisions of Bureau
of l 'dian Standards Act, 1986 and the Rules and Regulations made thereunder.
The details of conditions under which the licence for the use of Standard Mark
may be granted to manufacturers or producers may be obtained from the Bureau
of Indian Standards. '
(CRD 10)
Reprography Unit, BIS, New Delhi, India

INTERNATIONAL SYSTEM Of UNITS (81 UNITS)
8 ... Unit.
QuVttlty
Unl' Symbol
lenatJI
metre m
M..,
kilogram kg
nme .eeond I
"Electric current ampere A
Thermodynamic IIelvln K
temperature
luminoul Intenally candela cd
Amount of lub,tance mole mol
Supplem.ntary Unlb
Quantity
Unl'
Symbol
Plane angle radian rad
Solid angle steradian sr
Derived UnIts
Quantity Unl'
SymbOl DeflnlUoll
Force newton N N-1 kjl. m/a"
Energ, Joule J J - 1 N.m
Power
watt W W - 1 J/I
Flux
weber Wb 1 Wb -1 V.I
Flux denally
tella T 1 T -1 Wb/m"
Frequency
hertz Hz t Hz- 1 ell (a_I)
aeetrle conductanc.
lIemena
S S-1A/V
Electromotive force
volt
V 1
V - t W/A
Pr.alure, atre.s
pa,cal Pe Pa - 1 N/m"