SARS-Coronavirus

Classification,

Structure, Genome, Biology

- Nidovirales, Coronoviridae, Coronavirus

Zuckerman et al, 2009. Principles & Practice of Clin Virol. Wiley-Blackwell

Group 4

SARS-Coronavirus ?

- Virion (pleomorphic ): a. Single stranded positive-sense RNA b. Membrane-bound (covered with a distinctive fringe of widely spaced, club-shaped surface projections)

- Genome : 27-32 kb, which code : Structural proteins (S, E, M, N) & Non structural proteins (replicase polyprotein) Receptor : ACE2 - Host : animals (?) & humans SARS virus exists outside a human host - Human coronavirus : usually difficult to grow in tissue culture - More stable than other known human Coronaviruses Inactivated with : ether & chloroform, 56C
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 Epidemiology - Lower resp. tract infection - Hongkong, Vietnam, Taiwan, Singapura - Transmission : by droplet respiratorius, by direct & indirect contact by aerosolization of infected faeces ?? ~ animal market ?? - Super-spreaders - Moderately transmissible

MANDELL, DOUGLAS, BENNETTS. 2010. PRINCIPLES & PRACTICE OF INFECTIOUS DISEASES 7th ed Churchill Livingstone

 Clinical Manifestation - Incubation period : 2-14 days - Asymptomatic - Symptomatic (SARS = Severe Acute Respiratory Syndrome) : high fever (>38C), dry cough, shortness of breath

Lab Diagnosis
- Specimens : sputum, usap/washing tenggorok, stool - Virus isolation - Ab detection (ELISA) - Nucleic acid detection (RT-PCR)
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 Prevention (masker N95, handwashing)

Treatment : (antiviral : Ribavirin + Nelfinovir/Lopinavir,

antibacterial, symptomatic treatment)