Aeromonas salmonicida salmonicida A449 plasmid pAsa1, complete sequence

NCBI Reference Sequence: NC_004923.1 FASTA Graphics Go to:

LOCUS DEFINITION NC_004923 5424 bp DNA circular BCT 21-JUN-2010 Aeromonas salmonicida salmonicida A449 plasmid pAsa1, complete sequence. ACCESSION NC_004923 VERSION NC_004923.1 GI:32453760 DBLINK Project: 58631 KEYWORDS . SOURCE Aeromonas salmonicida subsp. salmonicida A449 ORGANISM Aeromonas salmonicida subsp. salmonicida A449 Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales; Aeromonadaceae; Aeromonas. REFERENCE 1 (bases 1 to 5424) AUTHORS Boyd,J., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M. TITLE Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449 JOURNAL Plasmid 50 (2), 131-144 (2003) PUBMED 12932739 REFERENCE 2 (bases 1 to 5424) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (07-JUL-2003) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 5424) AUTHORS Boyd,J.M., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M. TITLE Direct Submission JOURNAL Submitted (16-MAY-2003) Institute for Marine Biosciences National Research Council of Canada, 1411 Oxford St., Halifax, NS B3H 3Z1, Canada COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY301063. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..5424 /organism="Aeromonas salmonicida subsp. salmonicida A449" /mol_type="genomic DNA" /strain="A449" /sub_species="salmonicida" /db_xref="taxon:382245" /plasmid="pAsa1" gene 1014..1256 /gene="relB" /locus_tag="ASA_pAsa101" /old_locus_tag="pAsa1_01" /db_xref="GeneID:1496836" CDS 1014..1256 /gene="relB" /locus_tag="ASA_pAsa101" /old_locus_tag="pAsa1_01" /note="RelB; Qin prophage; bifunctional antitoxin of the RelE-RelB toxin-antitoxin system/transcriptional repressor"

gene

CDS

gene

misc_RNA

gene

CDS

rep_origin gene

/codon_start=1 /transl_table=11 /product="bifunctional antitoxin/transcriptional repressor RelB" /protein_id="NP_861552.1" /db_xref="GI:32453761" /db_xref="GeneID:1496836" /translation="MGTINLRIDDELKTRSYAVLERLGVTPSELLRQTLEYVASQERL PFRTTVVTEDEEALLVLVRERLASPQKGIRVSLDDL" 1246..1533 /gene="relE" /locus_tag="ASA_pAsa102" /old_locus_tag="pAsa1_02" /db_xref="GeneID:1496837" 1246..1533 /gene="relE" /locus_tag="ASA_pAsa102" /old_locus_tag="pAsa1_02" /note="RelE" /codon_start=1 /transl_table=11 /product="toxin" /protein_id="NP_861553.1" /db_xref="GI:32453762" /db_xref="GeneID:1496837" /translation="MTFELEFDPRAWKEWKKLGDTVRQQFKKKLAEVLERPRVEANRL HGLPDCYKIKLRGAGYRLVYQVQDDRVLVFVVAVGKREREQVYLDAGYRLE" complement(1578..1649) /locus_tag="ASA_pAsa1s1" /old_locus_tag="pAsa1_s1" /db_xref="GeneID:3722523" complement(1578..1649) /locus_tag="ASA_pAsa1s1" /old_locus_tag="pAsa1_s1" /product="RNAI" /note="antisense RNA; regulator of rep" /db_xref="GeneID:3722523" 1717..2724 /gene="rep" /locus_tag="ASA_pAsa103" /old_locus_tag="pAsa1_03" /db_xref="GeneID:1496838" 1717..2724 /gene="rep" /locus_tag="ASA_pAsa103" /old_locus_tag="pAsa1_03" /note="Rep; ColE2-type replication protein" /codon_start=1 /transl_table=11 /product="replication primase" /protein_id="NP_861554.1" /db_xref="GI:32453763" /db_xref="GeneID:1496838" /translation="MVKPALQAAEFVERLPLRPYCTDDPALGLHIRPQATALAFRHIQ HNPPPYVSCIVFDVDRKPYEQRREGYQEWRERGLPAPHWIAINPENGNYHLGYLLAAP VARTNAARLKPLRYLAAIEHVLAKKLGADMGYVGLITKNPVHSDWWTVWHNHAPYSLD YLAEFCPDADLAAYNRRSGKEASGLGRNVTVFDNVREWGYSAVREHWRPNGYDAWADA VRAACESANAFGREQGGPLPHSEIKATAKSIARWIWRHLTPTSFADYVDRTHTPEIQA RRGAKGGKVSKGGGRPSKAAELLPEVLRLKAMGYSNRDIAEDLQISAGSVSNYLRCDL E" 2736..2755 /note="oriV region" complement(2840..3163)

CDS

oriT gene

CDS

gene

CDS

/locus_tag="ASA_pAsa104" /old_locus_tag="pAsa1_04" /db_xref="GeneID:3722524" complement(2840..3163) /locus_tag="ASA_pAsa104" /old_locus_tag="pAsa1_04" /note="Orf1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="NP_861555.1" /db_xref="GI:32453764" /db_xref="GeneID:3722524" /translation="MKDTTDQGTGDMFGIKRGRGRPKTGAAKSGAERQAAYRAKLADI NVTVTINRELIDGLSVHLQAIRDGWSEVPLTPEQAGEILKALRTAELKQLRGQHPRPQ AKPTA" 3260..3278 /note="origin of transfer" 3372..3782 /gene="mobC" /locus_tag="ASA_pAsa105" /old_locus_tag="pAsa1_05" /db_xref="GeneID:1496839" 3372..3782 /gene="mobC" /locus_tag="ASA_pAsa105" /old_locus_tag="pAsa1_05" /note="MobC" /codon_start=1 /transl_table=11 /product="mobilization protein" /protein_id="NP_861556.1" /db_xref="GI:32453765" /db_xref="GeneID:1496839" /translation="MSKVQKITTVSVRIDKPTAAEWRERASASGLSLSDWIRGAVDAN QQTNLPTPQRRPVRVRDTSNDADPALMRQLAGMSNNLNQIARALNECRLAGSSVQLVE VLALLRSIEVQAGRLLPQLPPPPKPMAKGRDDAH" 3772..5424 /gene="mobA" /locus_tag="ASA_pAsa106" /old_locus_tag="pAsa1_06" /db_xref="GeneID:1496840" 3772..5424 /gene="mobA" /locus_tag="ASA_pAsa106" /old_locus_tag="pAsa1_06" /note="MobA" /codon_start=1 /transl_table=11 /product="mobilization relaxase" /protein_id="NP_861557.1" /db_xref="GI:32453766" /db_xref="GeneID:1496840" /translation="MLIKFLSHGTGSGARASAYLMGSHDHTGAPRAGVAVQRGDPVLF AAVADSLPFQHRYASAVISWATEEAPTADEITAVLEDFEAVAFAGLEPEDVHLTAVQH DEPDGGVHVHILIPRVHLGTGKSFNPAPPGHRKAFDAVRDKHNHEKGWARPDDPLRAR LLRPDFEAFKDKSDKSAIKKQITEHLLGAAAQGVISNAAELQDYLQTSLGCEITRTGA DYTSIKPEGYPKAIRLTGELYGSSWTAERTLEREAQTAARAGIGRGGEVSESGARQAQ ERLRDACGRRAEYNRGRYPGRDKGIAQTDHRLDRPIEGGLGAGPERGQHPQADLQRLA QSLAVRGMHLRGDRAGELGGIELDGQRGSAVEQRDQQRESHAGAASGRSELRPGGWQE LHPRSDIGKNVRNAKWQDGGGDQVNDRIRTPTDRAAGADGAAAPQRDDGLAAAALRAT DVFGELAKAVGEHLGRVRDALRQLTHRIRGLGEEHKERASQHGPEFGPDQPGYRSSTW VIEQQCGRSQELAGELDQGAALIADRAAELRKEQRRDRGLSM"

gene

CDS

gene

CDS

4487..5020 /gene="mobB" /locus_tag="ASA_pAsa107" /old_locus_tag="pAsa1_07" /db_xref="GeneID:1496841" 4487..5020 /gene="mobB" /locus_tag="ASA_pAsa107" /old_locus_tag="pAsa1_07" /note="MobB" /codon_start=1 /transl_table=11 /product="mobilization protein" /protein_id="NP_861558.1" /db_xref="GI:32453767" /db_xref="GeneID:1496841" /translation="MDHLGQQKERLSERLKQRQEQELGEVEKLAKAVLDKLKKDLETR VDGELNTTVDAIRDGIKGLPKQITDSTDPLKEVLEQARSEANTLKLISSVWLKALLFG VCISVGIGLGSWGVLSWMGNEVQRLNSEISSAKATLAQLPAGVSFVREDGKSYILAPT LGKTYETQSGKMAVEIK" 5021..5362 /gene="mobD" /locus_tag="ASA_pAsa108" /old_locus_tag="pAsa1_08" /db_xref="GeneID:1496842" 5021..5362 /gene="mobD" /locus_tag="ASA_pAsa108" /old_locus_tag="pAsa1_08" /note="MobD; putative" /codon_start=1 /transl_table=11 /product="mobilization protein" /protein_id="NP_861559.1" /db_xref="GI:32453768" /db_xref="GeneID:1496842" /translation="MTELERLLIERLEQMELRHHNETMDLRQQLYAQQMSLESLQRQL GSTLEGYETHCASLHTAFVALEKSTRSELASMGRSLDQTNRVTGAVLGSLNSSVAGVK SSLESLIKAQR" tatatcgtca tcctgacacg tcaaggctcc catcgactgg gcgccagcat cgatgcaggt tgtggcatac ctgcggactg tggcggcatg tgccgcgctg ctatagtggc acatctaaga gggtcacgat taagaaattt acaaaaacta ggatgaaggt tagagaatac cgattaacct ttggtgtaac gactgccttt gtgagcggct gagttcgacc ttcaaaaaga cagtgacaat gtggcgcata ccgtggctat ctgatcgatg gtggcccact gtggaggtgg ggtggcggta gttgggcctt gatagcgttg cgcttggcgg tcgtaatttt cacattaatg taccgcgacg tattatgatg actctaatgg attcaacagc gctaatatgt tcgcatcgat tccatctgag tcggacgacc ggccagcccc cacgagcgtg agctggcaga aattaaggag ttcgccagtt gggccgatgg acgacggcag atgccctgtt attaaggtgc ctggccgccg gggccgctct ttggccctgc ccactatagc gttcatctaa atgaagagtt cgtttaaaat acactcacaa cctatgtcag atcttaatga aagtacaaac gatgagctga ttactgcgac gtggtaacgg cagaaaggga gaaggaatgg ggtgctggag atcgggtatg tcccccggtt aaagccgctg ccgctggatc gtccgccgat gattgagtga tgttcgcggc acagcgcctt tgctgcggtg gggcgcgaca caaccataaa tagcccttta attgcgagag aatctctact cataggagaa tattttttat gtacttacac aaacccgctc aaactctgga aggatgagga tcagggtgtc aagaagttgg cgacctaggg aaatcaggtc gggtacgaac atcgacctcc gtcattgata acatggcggc ttggtttccg gaaagccctg ggcggggcct gatgaaggtc gactgcgcta ggacttcgaa ctgcttgccg caaggttgtt gggttagaga tacctagcta caagtagttg ctggggtttg gtatgcggta atatgtggcc ggcattgctt gctggatgac gcgatacagt tagaggcgaa ggttgggtac tggcgagcta tcgatgcgac ccgagtgcct aacggctgct gggctgatgc ctgtactgtg tcttgggacg tagcccgttc gtcgtgtccg tggaaggcac cttgcatcac ctttgataaa gcgtaaacca aaactggggg agcttaaaaa gatatgggga cttgaacgac tctcaggaac gttcttgttc ctttgagctt tcgccagcag tcgtctgcat

ORIGIN 1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321

1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101

gggctgccgg gtacaggatg gtgtatctgg ggtaacgtac acaacagagg cagacaaccc gccgaattcg ctgcatatcc ccgtatgtct gggtatcaag gaaaacggca gccaggctaa ggcgctgaca acggtatggc gcggatttag gtcactgtgt cccaatggat tttggacgcg atcgcccgtt acacataccc gggggcaggc ggctacagca tacctccgtt gggcgttcct ccgaaccgta ggagctgttt ttaatggtac tcagctctct cttgccgttc tgccaaacat ctgtaacggt tttttttgct gctcaacgta ccggaggccg cgaccgccgc tccggggggc tccgtgtccg tgagcaacaa cggtgcaact tgctgcccca aaattccttt cacgaccaca tttgcggcgg tgggcgacgg gcggtggcgt gaaccggatg aaatcgttca cacaaccacg cccgactttg gaacacctac tacctacaaa aagcctgagg acagcagaaa ggtggagaag ggacggcgag acagatcacc caacaccctc ctccgtgggg gcggttgaac gagcttcgtc cgaaacgcaa actgatcgag gcagcagctc

actgctacaa atcgggtgct acgctggtta tgtccagaaa attcggggcc agcaccgcca tcgaacggct gcccgcaggc cctgcattgt agtggcgaga actaccatct agccactgcg tggggtacgt ataaccatgc cggcctacaa tcgataacgt acgacgcatg agcagggcgg ggatctggcg ccgagatcca ccagcaaagc accgcgatat gtgatcttga ttatccctcc ggccgtccat tagttctgcc ttctgaccat atttattgtc tgccccgctt gtccccagta aattgattat gtactggtta acacggggct cgtgtccaag cgaatggcgt ggtggatgcc cgataccagc cctcaaccag ggtggaagtg gttgccgcca cccacggtac caggtgcacc tggccgactc aagaggcccc ttgctgggtt gcggggttca acccggcccc aaaagggctg aggcgttcaa ttggtgccgc catcgctcgg gctatcccaa gaacgcttga ttagcgaaag ctgaatacaa gactcgaccg aagctgatct atcgggctgg agcgagatca cgggaggatg agtggcaaga cggctggagc tacgcgcaac

gattaagctg ggtcttcgtg tcggctggaa agacaaaccc gttcactaag ataagcgcgg ccccctccgc aactgcgctg gtttgatgtg gcgtggcttg tggctatctg ctatctagcg tgggttgatc gccctattcc ccgccgcagc gcgggaatgg ggcggatgcg cccgttgccc gcaccttacc agcgcggaga ggcagagctg agccgaggat gtgatcaaaa agtatttgag caggccgtgg gttcttagcg ccatccctga accgtgacgt ttggctgctc ccctgatccg gtgtcatcgt ttagccagcc ggtgaggggc gtccaaaaga gaacgtgcta aatcagcaga aacgatgccg atagcccgag ctggccctgc cccccaaagc gggcagcggt acgggcaggt gcttccgttc gaccgccgac ggagccggaa cgtccacatc accaggtcac ggcgcggcca ggacaagagc cgcgcagggg gtgcgaaatc agctatccgg gcgagaggct cggtgctcga ccgtggacgc acccattgaa ccagcgtctg ggagctgggg gcagcgcgaa gcaagagtta tggcggtgga agatggagct agatgtcttt

cgtggggctg gtcgcagtgg taggtgaagt cgaatctctg aacaggaaaa tgatcgatgg ccctactgca gccttccgtc gacagaaagc cctgcgccgc ctggcagcgc gctatagagc accaaaaacc ctcgactacc ggcaaagagg ggctacagcg gtgagagctg catagtgaga ccgacctcgt ggggctaagg ctgccggaag ctgcaaatct acaagcctat agagaggctc gtttggcctg ccttcaagat tcgcctgcaa taatatctgc ctgtctttgg tggtgtcctt gacgataatt cacccgtatt tatgcccctt taaccaccgt gcgcctctgg cgaatttgcc atccggcgtt ctctgaacga tgcgcagcat ctatggccaa gcccgtgctt gtggcggtac cagcatcgtt gagattactg gacgtccacc ctgatccccc cgtaaagcct gacgacccgc gacaagagcg gtgatttcca acgcgcacag ttaacagggg caaacagcgg caagctcaag tatccgggac ggaggtcttg gctcaaagcc ggtattgagc agccacgctg catcctcgct gatcaagtaa gcggcaccac ggagagcttg

ggtaccggtt gtaaacgaga tgccgtgggg tttagtcaac gcataacata taaaaccagc ccgacgatcc acattcagca cctatgagca attggatcgc cagtggcgcg acgtgctggc ccgtacacag tcgccgagtt ctagtgggct cagtgcggga cctgtgagag tcaaggcaac ttgcggatta gcggcaaggt tattgcggct cggctggcag atcagataac ggtgcgaggt tgggcggggg ctcgccagcc atgtactgac caattttgca cctaccccgt cattgtgcta agcaagttgc acgttttagc caatccccca cagcgtcagg actcagtttg gacgccccag gatgcgccag atgccggttg agaggtacag agggcgtgac cggcctacct agcggggtga acgccagcgc cggttttgga tgacggcggt gtgttcacct ttgatgcggt tccgggcgcg ccatcaaaaa atgcggcgga gggccgatta aactctatgg caagagcagg aaagacttag gggataaagg gagcaggccc ttgctgttcg tggatgggca gcgcagcttc ccgacattgg atgaccgaat aacgagacga caaaggcagt

ggtgtatcag gcgggagcag tggctagtac ttggcgggag tgcacgttct gcttcaagcc ggcgttgggt caacccaccg gcggcgagaa gatcaacccc taccaatgca gaagaagctg cgattggtgg ctgcccagat ggggcgtaac gcattggcgg cgccaatgcg tgccaagagt cgttgatcgg atccaagggc caaagcaatg tgtgtccaat agcgcccgga gggcaagcgc tgttgtccac tgctctgggg agcccatcta cggtaggctg ccgcgcttga tttcctgtca cgcccctctc ctgcttcgcg cacaccacag atcgacaagc tctgactgga cgccgtcccg ttggccggga gccggctcgt gcgggacgcc gatgctcatt catgggctcg tccggtgttg agtgatctcg ggacttcgag ccagcacgac gggcacaggc gagggataaa gttgctacgg gcagattacc gctgcaggac caccagcatc atcatcttgg aattgggcga agacgcgtgt gattgcccaa ggagcgaggc gggtatgcat acgaggttca cggccggagt gaaaaacgta tagaacgcct tggacttgcg tggggagcac

5161 5221 5281 5341 5401

cttggaaggg gagcacaagg cggagcagta cttgatcaag agagatcgtg

tacgagacgc agcgagctag cttgggtcat gcgcagcgtt ggttgagcat

actgcgccag ccagcatggg tgaacagcag gatcgccgac gtga

cttacacacc ccggagtttg tgtggccgga agggctgcag

gcattcgtgg gaccagacca gtcaagagct agctgcgaaa

ccttggagaa accgggttac cgctggagag agagcagcgg

>gi|32453760:1649-2756 Aeromonas salmonicida salmonicida A449 plasmid pAsa1, complete sequence CCTATATCAGATAACAGCGCAGAACAGGAAAAGCATAACATATGCACGTTCTCAGACAACCCAGCACCGCCAATAAGCGCGGTGATCGA TGGTAAAACCAGCGCTTCAAGCCGCCGAATTCGTCGAACGGCTCCCCCTCCGCCCCTACTGCACCGACGAT CCGGCGTTGGGTCTGCATATCCGCCCGCAGGCAACTGCGCTGGCCTTCCGTCACATTCAGCACAACCCAC CGCCGTATGTCTCCTGCATTGTGTTTGATGTGGACAGAAAGCCCTATGAGCAGCGGCGAGAAGGGTATCA AGAGTGGCGAGAGCGTGGCTTGCCTGCGCCGCATTGGATCGCGATCAACCCCGAAAACGGCAACTACCAT CTTGGCTATCTGCTGGCAGCGCCAGTGGCGCGTACCAATGCAGCCAGGCTAAAGCCACTGCGCTATCTAG CGGCTATAGAGCACGTGCTGGCGAAGAAGCTGGGCGCTGACATGGGGTACGTTGGGTTGATCACCAAAAA CCCCGTACACAGCGATTGGTGGACGGTATGGCATAACCATGCGCCCTATTCCCTCGACTACCTCGCCGAG TTCTGCCCAGATGCGGATTTAGCGGCCTACAACCGCCGCAGCGGCAAAGAGGCTAGTGGGCTGGGGCGTA ACGTCACTGTGTTCGATAACGTGCGGGAATGGGGCTACAGCGCAGTGCGGGAGCATTGGCGGCCCAATGG ATACGACGCATGGGCGGATGCGGTGAGAGCTGCCTGTGAGAGCGCCAATGCGTTTGGACGCGAGCAGGGC GGCCCGTTGCCCCATAGTGAGATCAAGGCAACTGCCAAGAGTATCGCCCGTTGGATCTGGCGGCACCTTA CCCCGACCTCGTTTGCGGATTACGTTGATCGGACACATACCCCCGAGATCCAAGCGCGGAGAGGGGCTAA GGGCGGCAAGGTATCCAAGGGCGGGGGCAGGCCCAGCAAAGCGGCAGAGCTGCTGCCGGAAGTATTGCGG CTCAAAGCAATGGGCTACAGCAACCGCGATATAGCCGAGGATCTGCAAATCTCGGCTGGCAGTGTGTCCA ATTACCTCCGTTGTGATCTTGAGTGATCAAAAACAAGCCTATATCAGATAACAGCGCC