Determining quality components in Indian mustard by NIRS

Font, R., Del Ro, M., Fernndez-Martnez, J.M., De Haro, A. Instituto de Agricultura Sostenible ( SI !. Ala"eda del #bis$o s%n. &'()( *rdoba. S$ain.

Indian "ustard (Brassica juncea +. zern. , oss.! is an oilseed s$ecies -.ic. is gro-n in large areas o/ t.e Indian subcontinent. 0.is s$ecies is tolerant to droug.t and could be used as an oilseed cro$ in Mediterranean conditions (Fereres et al., &1)2!. 3ear in/rared s$ectrosco$4 (3IRS! is -idel4 acce$ted /or t.e ra$id and non-destructi5e anal4sis o/ se5eral 6ualit4 $ara"eters in 7rassica s$ecies (Daun et al., &118!. Rein.ardt and R9bbelen (&11&! and :elasco et al. (&118 and &11;! .a5e in5estigated t.e $ossibilit4 o/ using 3IRS to esti"ate indi5idual /att4 acids and &(((-seed -eig.t in <t.io$ian "ustard (Brassica carinata 7raun!. #ur ob=ecti5es in t.is stud4 are> &! to increase t.e 5ariabilit4 o/ ger"$las" collections o/ Indian "ustard co"ing /ro" India, France and ?SA t.roug. genetic i"$ro5e"ent, and @! to de5elo$ calibration e6uations /or deter"ining t.e contents o/ se5eral 6ualit4 co"$onents in B. juncea. A total o/ 2&@ intact and undried sa"$les o/ Indian "ustard seed -ere scanned in a 3IRS S4ste"s A8(( "onoc.ro"ator. 0.e s$ectra obtained -ere di5ided in t-o di//erent grou$s> a calibration set o/ @A( sa"$les and a 5alidation set o/ A(. 3ine 6ualit4 $ara"eters (t.e content o/ /att4 acids> erucic, oleic, linolenic, linoleic, stearic, $al"itic and eicosenoic, t.e content o/ oil, and -eig.t o/ &((( grains! -ere deter"ined b4 standard c.e"ical "et.ods (Bas-+i6uid ro"atogra$.4 and 3uclear Magnetic Resonance!. Crediction e6uations -ere obtained /or all $ara"eters. In eac. case t-o di//erent "at.e"atical treat"ents (&,',',& and @,8,8,&! -ere tested. <stndar 3or"al :ariation and Detrend scatter corrections -ere used, and a seg"ent /ro" &&(( to @8(( n" e5er4 ' n" -as considered in t.e calibration. Modi/ied $artial least s6uares (MC+S! -as t.e regression "et.od selected. 0.e calibration e6uations -ere 5alidated -it. a eDternal grou$ o/ sa"$les and t.e e6uations -it. t.e s"allest standard error o/ $er/or"ance (S<C! and t.e .ig.est coe//icient o/ deter"ination (r @ ! -ere selected. 0.e best calibration e6uations -ere obtained -it. t.e /irst deri5ati5e o/ s$ectra /or oil content and t.ousand-seed -eig.t, and -it. t.e second deri5ati5e /or erucic, stearic, oleic, eicosenoic, $al"itic, linolenic and linoleic acids content (0able &!. 0.e standard error o/ calibration (S< ! 5alues /or /att4 acids and oil content -ere under t.e li"it o/ (.)2 (eDce$t erucic, eicosenoic and oleic acids ! t.at is usuall4 considered satis/actor4 /or t.e anal4sis o/ ra$eseed sa"$les /or $lant breeding $ur$oses (0Eac.uE, &1)&!. #n t.e ot.er .and, t.e S< 5alues obtained in B. juncea calibration e6uations /or $al"itic, oleic, linoleic and erucic acids -ere lo-er t.an t.ose obtained in B. carinata (:elasco et al., &118! and .ig.er t.an /or oil content and &(((-seed -eig.t. 0.e S<C 5alues obtained /or oleic and eicosenoic acids and oil content in B. juncea -ere si"ilar to t.ose re$orted in B. carinata (:elasco et al., &118!. 0.e best coe//icients o/ deter"ination (r @! /or t.e eDternal 5alidation -ere obtained /or erucic and oleic acids and oil content ((.11, (.1) and (.1) res$ecti5el4!, -.ile t.e r @ /or &(((-seed -eig.t -as (.;) (0able @!. 0.ese results s.o- t.at 3IRS tec.ni6ue can be used -it. enoug. accurac4 in t.e screening o/ a large nu"ber o/ sa"$les o/ Brassica juncea in breeding $rogra""es /or 6ualit4 co"$onents in t.e seed. Acknowledgements 0.is -orE .as been su$$orted b4 t.e .I. .F.0. (Cro=. ABF18-(@@@! o/ t.e S$anis. Bo5ern"ent. References
Daun, J. ., and !.". #illiams. $%%&. ?se o/ 3IR S$ectrosco$4 to deter"ine 6ualit4 /actors in .ar5est sur5e4 o/ canola. $. )A'-)AA. In Croc. Int. Ra$eseed ongr., 1t., a"bridge, ?G. '-; Jul4 &118. Henr4 +ing, +td., Dorc.ester, ?G.

'ereres, (., J.). 'ern*nde+,)art-ne+, I. )ingue+, and J. Dom-ngue+. $%./. Croducti5it4 o/ Brassica juncea and B. carinata in relation to ra$eseed. Croc. At. Int. Ra$eseed ongr., Caris, France, @12-@1). Rein0ardt, 1.". and R2bbelen, 3. 4$%%$5 . Huantitati5e anal4sis o/ /att4 acids in intact Ra$eseed b4 3ear In/rared Re/lectance S$ectrosco$4. Croc. )t. Int. Ra$eseed ongr. anada. $$. &2)(-&2)'. 1kac0uk, R. $%.$. #il and $rotein anal4sis o/ -.ole ra$eseed Eernels b4 near in/rared re/lectance s$ectrosco$4. J. A". #il .e". Soc. 8)> )&1-)@@. 6elasco, 7., 'ern*nde+,)art-ne+, J.)., De 8aro, A. and Del Rio, ). $%%&. 0.e a$$licabilit4 o/ 3IRS /or esti"ating "ulti$le seed 6ualit4 co"$onents in <t.io$ian "ustard. 1t. International Ra$eseed ongress. a"bridge, ?G. &118. )A;-)A1. 6elasco, 7., 'ern*nde+,)art-ne+, J.). and De 8aro, A. $%%9. ?se o/ 3ear In/rared Re/lectance S$ectrosco$4 to Screen <t.io$ian Mustard /or Seed Ieig.t. Agron. J. )1: &8(-&82.

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r@K oe//icient o/ deter"ination. S<CK Standard error o/ $er/or"ance.