CCH TIN HNH

Nguyn liu mi loi 100g,

1. Sy kh, nghin th
2. Ngm chit 20g bt th vi 150ml ethanol 80o (ch trc khi tin hnh
chit lm m bng khong 10 ml mi, trong 30 pht ri mi chit) trong
48 gi
3. Thu hi dung mi + 50 ml nc nng hn dch
4. Lc hn dch vi n-hexan (3 ln, mi ln vi 30 ml)
5. Phn nc cn li phn 4, lc etyl axetat 3 ln (mi ln dng 50 ml ethyl
acetat). Dch chit ethyl acetat, em thu hi dung mi, c cn. Cn cn
thu c.
6. Cn ny dng nh tnh bng phn ng ho hc, SKLM v nh lng
flavonoid bng phng php quang ph UV-Vis.