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Our aim is the study of the physicochemical protein properties (volume,
compressibility) as a function of hydration by an ultrasonic technique. For
this purpose, we have used a biomimetic medium: reverse micelles, a
system where we can control precisely water concentration. The
compressibility of a medium is obtained by measuring the density and the
ultrasonic celerity. The difficulty is increased in a complex fluid: nanometric
size inclusions (micelles) dispersed in an organic solvent. To reach this goal,
we have custom-built in our laboratory an apparatus allowing the
determination of ultrasonic celerity, in very small volumes, with a relative
precision of about 10 ppm. Using the effective medium theory, we have
determined the compressibility of these inclusions with a precision better
than 1%. With a spherical model for micellar inclusions and with the
hydrodynamic radius of micelles obtained by x rays, we have estimated the
compressibility of the water within micelles. We have also evidenced a
difference in compressibility between the central core water and water
bound to the polar surfactant headgroups. Finally by applying the mean
field theory, we have obtained an estimate of the compressibility of
proteins inserted within the micelles, at various hydration levels.
PACS
43.80.Cs
Acoustical characteristics of biological media: molecular
species, cellular level tissues
43.80.Ev
Acoustical measurement methods in biological systems
and media
ARTICLE DATA
PUBLICATION DATA
ISSN:
0001-4966 (print)
Ultrasonic characterization of proteins in complex fluids
J. Acoust. Soc. Am. Volume 109, Issue 5, pp. 2340-2340 (2001); (1 page)
Donato Valdez
1
, Marcel Gindre
1
, Jean-Yves Lehuerou
1
, Marcel Waks
1
, and Wladimir Urbach
2
1
Laboratoire d'Imagerie Parametrique, 15 rue de l'ecole de medecine, F-75006 Paris, France
2
Laboratoire de Physique Statistique, Paris, France
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