Schekman Lecture 3

Membrane Structure:
Dynamics
T3
Whitney Smith, Geneth Chin, Summitt Liu
Two Protein Linkages
Asparagine bonded to Acetylglucosamine Serine bonded to Acetylglucosamine
Fixed Membrane Proteins
Gold particles attached to ConA which attaches to
Mannose which binds to proteins.
Membrane
Binding to the gold allows the position of the
proteins to be seen using electron microscopy.
http://en.wikiversity.org/wiki/File:Gold_molecule-membrane.jpg
Protein Placement on Cell Membrane
1) Peripheral Protein
2) Peripheral Protein
3) Protein Spans across bilayer
4) Integral Protein
5) Peripheral Protein (to protein 4)
http://en.wikiversity.org/wiki/File:Cell_membrane_protein_diagram.jpg
SDS Gel Electrophoresis
Band of larger
proteins
http://en.wikipedia.org/wiki/File:SDS-PAGE.jpg
Bands of smaller
proteins
Immunoblotting (Western Blot Technique)
Proteins transfer onto filter paper at the bottom of
the gel
https://ww2.chemistry.gatech.
edu/~lw26/bCourse_Information/4581/techniques/gel_elect/gel.
jpg
Nitrocellulose Filter Paper
http://en.wikiversity.org/wiki/File:SDS-PAGE_Coomasie_stained.jpg
Hydropathy Plot for Glycophorin
http://cc.scu.edu.cn/G2S/eWebEditor/uploadfile/20120810131402735.jpg
Glycophorin
Hydropathy Plot used to gain information
about structure of protein
Bacterial Photoreaction Center
Crystal structure for photoreaction center
http://en.wikipedia.org/wiki/File:
First_satge_sbep2.svg
http://en.wikipedia.org/wiki/File:
Reactprotdatbnk.JPG
Photoreaction Center Diagram