Plot of Absorbance vs Time

Set 1
Set 2
Set 3
Set 4
Set 5

0.6
0.5

Absorbance

0.4

Set 1: y = 0.0489x + 0.2038

R = 0.9852

0.3

Set 2: y = 0.0698x + 0.2496

R = 0.998

0.2

Set 3: y = 0.1062x + 0.291

R = 0.9961

0.1

Set 4: y = 0.1548x + 0.3903

R = 0.9874
Set 5: y = 0.1509x + 0.391
R = 0.9408
1.2

0
0

0.2

0.4

0.6

0.8

Time (min)

Plot of Absorbance vs Time With an Inhibitor

Set 1: y = 0.057x + 0.3207
R = 0.9882

0.7

Set 2: y = 0.0657x + 0.2338

R = 0.9975
Set 3: y = 0.1128x + 0.3559
R = 0.9971
Set 4: y = 0.0943x + 0.3247
R = 0.9839
Set 5: y = 0.1374x + 0.5274
R = 0.998

0.6

Absorbance

0.5
0.4
0.3
0.2
0.1
0

0.2

0.4

0.6
Time (min)

0.8

1.2

Set 1
Set 2
Set 3
Set 4
Set 5

Michaelis-Menten Plot of
Tyrosinase-Catalyzed Reactions
0.05

Without Inhibitor
With Inhibitor

0.045

Without Inhibitor:
y = 0.01x + 0.0054
R = 0.9311

0.035
0.03
0.025
0.02

With Inhibitor:
y = 0.0066x + 0.0101
R = 0.8237

0.015
0.01
0.005
0
0

Substrate Concentration [S] (mM)

Lineweaver-Burk Plot of
Tyrosinase-Catalyzed Reactions
Without Inhibitor

1/Vo (min/mM)

Vo (mM/min)

0.04

90
80
70
60
50
40
30
20
10
0

With Inhibitor
Without Inhibitor:
y = 51.572x + 11.802
R = 0.9531

With Inhibitor:
y = 34.534x + 23.193
R = 0.8095
0

0.5

Recipricol of Substrate Concentration 1/[S]

1.5
(mM-1)